Evidence: homology, This is single molecule of human insulin analogue consisting of A and B chains inter-linked by inter-molecular disulphide bonds A7-B7 and A20-B19. There is one A chain intra-chain ...Evidence: homology, This is single molecule of human insulin analogue consisting of A and B chains inter-linked by inter-molecular disulphide bonds A7-B7 and A20-B19. There is one A chain intra-chain disulphide A6-A11 as well.
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
Buried area
1460 Å2
ΔGint
-26 kcal/mol
Surface area
3400 Å2
Method
PISA
Unit cell
Length a, b, c (Å)
58.460, 58.460, 56.360
Angle α, β, γ (deg.)
90.00, 90.00, 90.00
Int Tables number
97
Space group name H-M
I422
-
Components
#1: Protein/peptide
InsulinBchain
Mass: 2953.399 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This is modified B chain of human insulin. (i) Human sequence B26Tyr is replaced by trans-hydroxy-Proline (HYP). (ii) The last four B27-B30 residues of human insulin are truncated/deleted. ...Details: This is modified B chain of human insulin. (i) Human sequence B26Tyr is replaced by trans-hydroxy-Proline (HYP). (ii) The last four B27-B30 residues of human insulin are truncated/deleted. (iii) B26Hyp terminates with the carboxyamide group (-CONH2) instead of normal -COOH. Source: (synth.) Homo sapiens (human) / References: UniProt: P01308
#2: Protein/peptide
InsulinAchain
Mass: 2383.698 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This is the same sequence as for the wild type of human insulin A chain Source: (synth.) Homo sapiens (human) / References: UniProt: P01308