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- PDB-8kcn: X-ray structure of 3-alpha-(Or 20-beta)-hydroxysteroid dehydrogen... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8kcn | ||||||
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Title | X-ray structure of 3-alpha-(Or 20-beta)-hydroxysteroid dehydrogenase mutant | ||||||
![]() | NADP-dependent (R)-specific alcohol dehydrogenase | ||||||
![]() | OXIDOREDUCTASE / 3-alpha-(Or 20-beta)-hydroxysteroid dehydrogenase | ||||||
Function / homology | ![]() Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor / nucleotide binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Song, S.K. / Ma, Q.Q. / Lv, D.Q. / Cheng, C.Y. / Su, Z.D. | ||||||
Funding support | 1items
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![]() | ![]() Title: X-ray structure of 3-alpha-(Or 20-beta)-hydroxysteroid dehydrogenase mutant Authors: Song, S.K. / Ma, Q.Q. / Lv, D.Q. / Cheng, X.Y. / Su, Z.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63.6 KB | Display | ![]() |
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PDB format | ![]() | 45.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 408.5 KB | Display | ![]() |
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Full document | ![]() | 408.4 KB | Display | |
Data in XML | ![]() | 12.1 KB | Display | |
Data in CIF | ![]() | 17.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 26680.141 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.13 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, sitting drop / Details: 30% PEG MME550; 0.1M Tris pH7.0; 0.05M MgCL |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jan 1, 2023 |
Radiation | Monochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.31→65.365 Å / Num. obs: 60439 / % possible obs: 98.9 % / Redundancy: 1.9 % / CC1/2: 0.996 / Net I/σ(I): 14.9 |
Reflection shell | Resolution: 1.31→1.31 Å / Rmerge(I) obs: 0.1624 / Num. unique obs: 57490 / Rrim(I) all: 0.1792 / % possible all: 98.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 8.18 Å2
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Refinement step | Cycle: 1 / Resolution: 1.31→31.47 Å
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Refine LS restraints |
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