[English] 日本語
Yorodumi
- PDB-8kaj: Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 16nt... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 8kaj
TitleCrystal structure of SpyCas9-crRNA-tracrRNA complex bound to 16nt target DNA
Components
  • CRISPR-associated endonuclease Cas9/Csn1
  • DNA (5'-D(*CP*AP*AP*TP*AP*CP*CP*TP*TP*TP*TP*AP*TP*CP*CP*AP*TP*AP*AP*AP*TP*TP*CP*G)-3')
  • DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3')
  • RNA (34-MER)
  • RNA (65-MER)
KeywordsRNA BINDING PROTEIN/RNA/DNA / Nuclease / RNA BINDING PROTEIN-RNA-DNA complex
Function / homology
Function and homology information


maintenance of CRISPR repeat elements / 3'-5' exonuclease activity / DNA endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / DNA binding / RNA binding / metal ion binding
Similarity search - Function
CRISPR-associated endonuclease Cas9, PAM-interacting domain / CRISPR-associated endonuclease Cas9, bridge helix / CRISPR-associated endonuclease Cas9, REC lobe / REC lobe of CRISPR-associated endonuclease Cas9 / Bridge helix of CRISPR-associated endonuclease Cas9 / PAM-interacting domain of CRISPR-associated endonuclease Cas9 / CRISPR-associated endonuclease Cas9 / HNH endonuclease / Cas9-type HNH domain / Cas9-type HNH domain profile. ...CRISPR-associated endonuclease Cas9, PAM-interacting domain / CRISPR-associated endonuclease Cas9, bridge helix / CRISPR-associated endonuclease Cas9, REC lobe / REC lobe of CRISPR-associated endonuclease Cas9 / Bridge helix of CRISPR-associated endonuclease Cas9 / PAM-interacting domain of CRISPR-associated endonuclease Cas9 / CRISPR-associated endonuclease Cas9 / HNH endonuclease / Cas9-type HNH domain / Cas9-type HNH domain profile. / HNH nuclease / Ribonuclease H superfamily
Similarity search - Domain/homology
DNA / DNA (> 10) / RNA / RNA (> 10) / CRISPR-associated endonuclease Cas9/Csn1
Similarity search - Component
Biological speciesStreptococcus pyogenes serotype M1 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.42 Å
AuthorsChen, Y. / Chen, J. / Liu, L.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32022047 China
CitationJournal: Nat.Biotechnol. / Year: 2024
Title: Trans-nuclease activity of Cas9 activated by DNA or RNA target binding.
Authors: Chen, J. / Chen, Y. / Huang, L. / Lin, X. / Chen, H. / Xiang, W. / Liu, L.
History
DepositionAug 3, 2023Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Jun 5, 2024Provider: repository / Type: Initial release
Revision 1.1Jun 12, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

-
Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
A: RNA (34-MER)
B: CRISPR-associated endonuclease Cas9/Csn1
C: DNA (5'-D(*CP*AP*AP*TP*AP*CP*CP*TP*TP*TP*TP*AP*TP*CP*CP*AP*TP*AP*AP*AP*TP*TP*CP*G)-3')
D: DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3')
E: RNA (34-MER)
F: CRISPR-associated endonuclease Cas9/Csn1
G: DNA (5'-D(*CP*AP*AP*TP*AP*CP*CP*TP*TP*TP*TP*AP*TP*CP*CP*AP*TP*AP*AP*AP*TP*TP*CP*G)-3')
H: DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3')
I: RNA (65-MER)
J: RNA (65-MER)


Theoretical massNumber of molelcules
Total (without water)402,25310
Polymers402,25310
Non-polymers00
Water00
1
A: RNA (34-MER)
B: CRISPR-associated endonuclease Cas9/Csn1
C: DNA (5'-D(*CP*AP*AP*TP*AP*CP*CP*TP*TP*TP*TP*AP*TP*CP*CP*AP*TP*AP*AP*AP*TP*TP*CP*G)-3')
D: DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3')
I: RNA (65-MER)


Theoretical massNumber of molelcules
Total (without water)201,1275
Polymers201,1275
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area19590 Å2
ΔGint-161 kcal/mol
Surface area81160 Å2
MethodPISA
2
E: RNA (34-MER)
F: CRISPR-associated endonuclease Cas9/Csn1
G: DNA (5'-D(*CP*AP*AP*TP*AP*CP*CP*TP*TP*TP*TP*AP*TP*CP*CP*AP*TP*AP*AP*AP*TP*TP*CP*G)-3')
H: DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3')
J: RNA (65-MER)


Theoretical massNumber of molelcules
Total (without water)201,1275
Polymers201,1275
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area18780 Å2
ΔGint-158 kcal/mol
Surface area80970 Å2
MethodPISA
Unit cell
Length a, b, c (Å)144.735, 131.105, 146.631
Angle α, β, γ (deg.)90.000, 103.680, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

-
Components

#1: RNA chain RNA (34-MER)


Mass: 10912.471 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: crRNA
Source: (synth.) Streptococcus pyogenes serotype M1 (bacteria)
#2: Protein CRISPR-associated endonuclease Cas9/Csn1 / SpCas9 / SpyCas9


Mass: 158588.781 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Streptococcus pyogenes serotype M1 (bacteria)
Gene: cas9, csn1, SPy_1046 / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: Q99ZW2, Hydrolases; Acting on ester bonds
#3: DNA chain DNA (5'-D(*CP*AP*AP*TP*AP*CP*CP*TP*TP*TP*TP*AP*TP*CP*CP*AP*TP*AP*AP*AP*TP*TP*CP*G)-3')


Mass: 7262.729 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: TS
Source: (synth.) Streptococcus pyogenes serotype M1 (bacteria)
#4: DNA chain DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3')


Mass: 3394.230 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: NTS
Source: (synth.) Streptococcus pyogenes serotype M1 (bacteria)
#5: RNA chain RNA (65-MER)


Mass: 20968.490 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: tracrRNA
Source: (gene. exp.) Streptococcus pyogenes serotype M1 (bacteria)
Production host: Escherichia coli DH5[alpha] (bacteria)

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

CrystalDensity Matthews: 3.46 Å3/Da / Density % sol: 64.41 %
Crystal growTemperature: 289 K / Method: vapor diffusion, hanging drop
Details: 1.0 M Sodium chloride, 0.1 M Sodium citrate pH 6.0, 12.75% PEG4000

-
Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.97853 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 24, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97853 Å / Relative weight: 1
ReflectionResolution: 3.38→50 Å / Num. obs: 97863 / % possible obs: 99.9 % / Redundancy: 6.1 % / Biso Wilson estimate: 52.3 Å2 / Rpim(I) all: 0.09 / Net I/σ(I): 8.71
Reflection shellResolution: 3.38→3.44 Å / Redundancy: 5.4 % / Num. unique obs: 3645 / Rpim(I) all: 0.493 / % possible all: 99.6

-
Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
HKL-30007.21data scaling
HKL-30007.21data reduction
Coot0.7.2.1model building
PHENIX1.17.1_360phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.42→48.03 Å / SU ML: 0.3864 / Cross valid method: FREE R-VALUE / σ(F): 1.39 / Phase error: 24.1207
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2465 3307 3.38 %
Rwork0.237 94556 -
obs0.2373 97863 69.09 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 68.16 Å2
Refinement stepCycle: LAST / Resolution: 3.42→48.03 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms21467 5496 0 0 26963
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.012227983
X-RAY DIFFRACTIONf_angle_d1.550938903
X-RAY DIFFRACTIONf_chiral_restr0.1094492
X-RAY DIFFRACTIONf_plane_restr0.00934028
X-RAY DIFFRACTIONf_dihedral_angle_d19.999811332
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.42-3.470.483930.342481X-RAY DIFFRACTION1.42
3.47-3.520.3678110.2818313X-RAY DIFFRACTION5.54
3.52-3.570.2627160.2914501X-RAY DIFFRACTION8.78
3.57-3.630.2494310.2746832X-RAY DIFFRACTION14.71
3.63-3.70.2955540.28971409X-RAY DIFFRACTION24.75
3.7-3.760.2937740.28682215X-RAY DIFFRACTION38.79
3.76-3.840.2388960.27252875X-RAY DIFFRACTION49.98
3.84-3.910.29451010.25653104X-RAY DIFFRACTION54.66
3.91-40.2461260.2593499X-RAY DIFFRACTION61.06
4-4.090.25231380.2453952X-RAY DIFFRACTION69.37
4.09-4.190.26791590.244343X-RAY DIFFRACTION76.06
4.19-4.310.25311540.23834600X-RAY DIFFRACTION81.92
4.31-4.430.28461800.25125081X-RAY DIFFRACTION89.11
4.43-4.580.23721960.24295409X-RAY DIFFRACTION94.49
4.58-4.740.22351920.24295597X-RAY DIFFRACTION97.69
4.74-4.930.23191940.2335560X-RAY DIFFRACTION98.43
4.93-5.150.25552020.22695692X-RAY DIFFRACTION99.28
5.15-5.430.25521950.22575674X-RAY DIFFRACTION99.54
5.43-5.760.21432010.22835697X-RAY DIFFRACTION99.54
5.76-6.210.24731910.23555668X-RAY DIFFRACTION99.68
6.21-6.830.25421930.24685686X-RAY DIFFRACTION99.73
6.83-7.820.22821960.23365690X-RAY DIFFRACTION99.63
7.82-9.830.23622030.20925688X-RAY DIFFRACTION99.68
9.84-48.030.23262010.21595390X-RAY DIFFRACTION94.79

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more