- PDB-8k2r: The structure of HtpG M domain in complex with unstructured D131D... -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: PDB / ID: 8k2r
タイトル
The structure of HtpG M domain in complex with unstructured D131D binding site b
要素
Disordered protein(D131D)
Molecular chaperone HtpG (Fragment)
キーワード
CHAPERONE / E.coli Hsp90
機能・相同性
機能・相同性情報
endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / ATP-dependent protein folding chaperone / unfolded protein binding / response to heat / nucleic acid binding / DNA damage response / perinuclear region of cytoplasm / ATP hydrolysis activity / extracellular region ...endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / ATP-dependent protein folding chaperone / unfolded protein binding / response to heat / nucleic acid binding / DNA damage response / perinuclear region of cytoplasm / ATP hydrolysis activity / extracellular region / ATP binding / membrane / metal ion binding / cytoplasm / cytosol 類似検索 - 分子機能
Thermonuclease family signature 1. / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily / Heat shock protein Hsp90, conserved site / Heat shock hsp90 proteins family signature. ...Thermonuclease family signature 1. / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily / Heat shock protein Hsp90, conserved site / Heat shock hsp90 proteins family signature. / HSP90, C-terminal domain / Heat shock protein Hsp90, N-terminal / Heat shock protein Hsp90 family / Hsp90 protein / Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase / Histidine kinase-like ATPases / Histidine kinase/HSP90-like ATPase / Histidine kinase/HSP90-like ATPase superfamily / Ribosomal protein S5 domain 2-type fold 類似検索 - ドメイン・相同性
タイプ: solution 内容: 500 uM [U-100% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-10% 13C; U-99% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-13C; U-15N; U-2H] Heat shock protein 90 (Hsp90) M ...内容: 500 uM [U-100% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-10% 13C; U-99% 15N] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-13C; U-15N; U-2H] Heat shock protein 90 (Hsp90) M domain, 500 uM [U-100% 13C; U-100% 15N] Disordered protein (D131D), 93% H2O/7% D2O Label: 13CH3_sample / 溶媒系: 93% H2O/7% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
500uM
Heatshockprotein90 (Hsp90) Mdomain
[U-100% 15N]
1
500uM
Heatshockprotein90 (Hsp90) Mdomain
[U-10% 13C; U-99% 15N]
2
500uM
Heatshockprotein90 (Hsp90) Mdomain
[U-13C; U-15N; U-2H]
3
500uM
Disorderedprotein (D131D)
[U-100% 13C; U-100% 15N]
4
試料状態
イオン強度: 150 mM / Label: condition_1 / pH: 7 / 圧: 1 Pa / 温度: 310 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker AVANCE III
Bruker
AVANCEIII
600
1
Bruker AVANCE III
Bruker
AVANCEIII
850
2
-
解析
NMR software
名称
開発者
分類
CNS
Brunger, Adams, Clore, Gros, NilgesandRead
精密化
CYANA
Guntert, MumenthalerandWuthrich
structurecalculation
NMRView
Johnson, OneMoonScientific
chemicalshiftassignment
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
解析
精密化
手法: molecular dynamics / ソフトェア番号: 1
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20