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Open data
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Basic information
Entry | Database: PDB / ID: 8k2p | ||||||
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Title | Crystal structure of CtGST-F76A | ||||||
![]() | Glutathione S-transferase | ||||||
![]() | TRANSFERASE / Deoxynivaneol detoxification enzyme / glutathione S-transferase / Fusarium head blight resistance | ||||||
Function / homology | Glutathione S-transferase, N-terminal domain / Glutathione S-transferase, C-terminal domain / Glutathione S-transferase, N-terminal / Glutathione S-transferase, C-terminal domain superfamily / transferase activity / Thioredoxin-like superfamily / Glutathione S-transferase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yang, J. / Xiao, J.Y. / Lei, X.G. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7. Authors: Yang, J. / Liang, K. / Ke, H. / Zhang, Y. / Meng, Q. / Gao, L. / Fan, J. / Li, G. / Zhou, H. / Xiao, J. / Lei, X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 212.1 KB | Display | ![]() |
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PDB format | ![]() | 169.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 443.1 KB | Display | ![]() |
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Full document | ![]() | 449.6 KB | Display | |
Data in XML | ![]() | 21.3 KB | Display | |
Data in CIF | ![]() | 30.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7yocC ![]() 7yp0C ![]() 8k2oC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 31311.475 Da / Num. of mol.: 2 / Mutation: F76A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.08 Å3/Da / Density % sol: 60.02 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 1.5 M Lithium sulfate, 0.1 M Trisodium citrate, pH 6.0 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Mar 17, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→66.78 Å / Num. obs: 37444 / % possible obs: 94 % / Redundancy: 13.1 % / CC1/2: 0.999 / Rmerge(I) obs: 0.093 / Rpim(I) all: 0.027 / Rrim(I) all: 0.097 / Χ2: 0.94 / Net I/σ(I): 18.7 / Num. measured all: 490916 |
Reflection shell | Resolution: 2.2→2.31 Å / Redundancy: 12.5 % / Rmerge(I) obs: 1.098 / Num. unique obs: 4096 / CC1/2: 0.868 / Rpim(I) all: 0.32 / Rrim(I) all: 0.097 / Χ2: 0.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→32.58 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -9.5024 Å / Origin y: 0.3062 Å / Origin z: 16.1329 Å
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Refinement TLS group | Selection details: all |