+Open data
-Basic information
Entry | Database: PDB / ID: 8jwx | |||||||||
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Title | bottom segment of the bacteriophage M13 mini variant | |||||||||
Components |
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Keywords | VIRAL PROTEIN / M13 | |||||||||
Function / homology | Function and homology information viral extrusion / virion attachment to host cell pilus / adhesion receptor-mediated virion attachment to host cell / helical viral capsid / host cell membrane / virion component / viral capsid / entry receptor-mediated virion attachment to host cell / symbiont entry into host cell / host cell plasma membrane / membrane Similarity search - Function | |||||||||
Biological species | Enterobacteria phage M13 (virus) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||
Authors | Xiang, Y. / Jia, Q. | |||||||||
Funding support | China, 2items
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Citation | Journal: To Be Published Title: Cryo-EM structure of a bacteriophage M13 mini variant Authors: Xiang, Y. / Jia, Q. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8jwx.cif.gz | 338 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8jwx.ent.gz | 272.6 KB | Display | PDB format |
PDBx/mmJSON format | 8jwx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8jwx_validation.pdf.gz | 430.1 KB | Display | wwPDB validaton report |
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Full document | 8jwx_full_validation.pdf.gz | 437.4 KB | Display | |
Data in XML | 8jwx_validation.xml.gz | 33.1 KB | Display | |
Data in CIF | 8jwx_validation.cif.gz | 52.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jw/8jwx ftp://data.pdbj.org/pub/pdb/validation_reports/jw/8jwx | HTTPS FTP |
-Related structure data
Related structure data | 8jwwC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 42573.766 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage M13 (virus) / Gene: III / Production host: Escherichia (bacteria) / References: UniProt: P69168 #2: Protein/peptide | Mass: 5243.014 Da / Num. of mol.: 15 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage M13 (virus) / Gene: VIII / Production host: Escherichia (bacteria) / References: UniProt: P69541 #3: Protein | Mass: 12357.984 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage M13 (virus) / Gene: VI / Production host: Escherichia (bacteria) / References: UniProt: P69532 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: bottom segment of the bacteriophage M13 mini variant / Type: COMPLEX / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: Enterobacteria phage M13 (virus) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1700 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 40 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: NONE |
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3D reconstruction | Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 45177 / Symmetry type: POINT |