National Natural Science Foundation of China (NSFC)
中国
引用
ジャーナル: Nat Commun / 年: 2023 タイトル: Cryo-EM structure of a bacteriophage M13 mini variant. 著者: Qi Jia / Ye Xiang / 要旨: Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM ...Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages.
F: Capsid protein G8P G: Capsid protein G8P H: Capsid protein G8P I: Capsid protein G8P J: Capsid protein G8P E: Capsid protein G8P D: Capsid protein G8P Q: Capsid protein G8P A: Capsid protein G8P B: Capsid protein G8P C: Capsid protein G8P K: Capsid protein G8P L: Capsid protein G8P M: Capsid protein G8P N: Capsid protein G8P O: Capsid protein G8P P: Capsid protein G8P R: Capsid protein G8P S: Capsid protein G8P T: Capsid protein G8P V: Capsid protein G8P W: Capsid protein G8P X: Capsid protein G8P Y: Capsid protein G8P Z: Capsid protein G8P AA: Capsid protein G8P BA: Capsid protein G8P CA: Capsid protein G8P DA: Capsid protein G8P EA: Capsid protein G8P FA: Capsid protein G8P GA: Capsid protein G8P HA: Capsid protein G8P IA: Capsid protein G8P JA: Capsid protein G8P KA: Capsid protein G8P LA: Capsid protein G8P MA: Capsid protein G8P NA: Capsid protein G8P OA: Capsid protein G8P