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- PDB-8jvb: Cryo-EM structure of the Type II secretion system protein from Ac... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8jvb | ||||||
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Title | Cryo-EM structure of the Type II secretion system protein from Acidithiobacillus caldus | ||||||
![]() | Type II and III secretion system protein | ||||||
![]() | TOXIN / Type II secretion system protein / Acidithiobacillus caldus | ||||||
Function / homology | Pilus formation protein, N-terminal / Pilus formation protein N terminal region / GspD/PilQ family / Type II/III secretion system / Bacterial type II and III secretion system protein / type II protein secretion system complex / protein secretion / Type II and III secretion system protein![]() | ||||||
Biological species | Acidithiobacillus caldus | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.39 Å | ||||||
![]() | Liu, R.H. / Zhang, K. / Feng, Q.S. / Dai, X. / Fu, Y. / Li, Y. | ||||||
Funding support | 1items
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![]() | ![]() Title: Cryo-EM structure of the Type II secretion system protein from Acidithiobacillus caldus Authors: Zhang, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 827.5 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
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Full document | ![]() | 1.5 MB | Display | |
Data in XML | ![]() | 123.7 KB | Display | |
Data in CIF | ![]() | 171.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 36673MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 53556.238 Da / Num. of mol.: 14 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: Atc_2749 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Type II secretion system protein / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 3200 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
CTF correction | Type: NONE | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 2.39 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 72834 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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