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- PDB-8jt3: Crystal structure of aminotransferase CrmG from Actinoalloteichus... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8jt3 | ||||||
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Title | Crystal structure of aminotransferase CrmG from Actinoalloteichus sp. WH1-2216-6 in complex with amino donor L-Arg | ||||||
![]() | CrmG | ||||||
![]() | TRANSFERASE / Transaminase / complex / amino donor | ||||||
Function / homology | ![]() transaminase activity / pyridoxal phosphate binding / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Su, K. / Zhang, Y. / Xu, J. / Liu, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Co-crystal structure provides insights on transaminase CrmG recognition amino donor L-Arg. Authors: Chen, R. / Su, K. / Zhang, Y. / Zhu, Y. / Liu, J. / Xu, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 402.1 KB | Display | ![]() |
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PDB format | ![]() | 330.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.2 MB | Display | ![]() |
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Full document | ![]() | 3.3 MB | Display | |
Data in XML | ![]() | 75.9 KB | Display | |
Data in CIF | ![]() | 103.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
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Components
#1: Protein | Mass: 57219.520 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() #2: Chemical | ChemComp-EQJ / ( #3: Chemical | ChemComp-GOL / #4: Chemical | ChemComp-ACT / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.42 Å3/Da / Density % sol: 49.07 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.2 M Sodium acetate, 0.1 M TRIS pH 8.5, 32%PEG 3350, 2% glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jan 5, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9785 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→40.19 Å / Num. obs: 84776 / % possible obs: 93.7 % / Redundancy: 3.8 % / CC1/2: 0.989 / Rmerge(I) obs: 0.129 / Rpim(I) all: 0.075 / Rrim(I) all: 0.15 / Net I/σ(I): 7.6 / Num. measured all: 322678 |
Reflection shell | Resolution: 2.35→2.39 Å / % possible obs: 92.6 % / Redundancy: 4 % / Rmerge(I) obs: 0.593 / Num. measured all: 17008 / Num. unique obs: 4290 / CC1/2: 0.777 / Rpim(I) all: 0.335 / Rrim(I) all: 0.685 / Net I/σ(I) obs: 2.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.151 Å2
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Refinement step | Cycle: 1 / Resolution: 2.35→40.19 Å
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Refine LS restraints |
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