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- PDB-8jt1: COLLAGENASE FROM GRIMONTIA (VIBRIO) HOLLISAE 1706B COMPLEXED WITH... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8jt1 | ||||||
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Title | COLLAGENASE FROM GRIMONTIA (VIBRIO) HOLLISAE 1706B COMPLEXED WITH GLY-PRO-HYP-GLY-PRO-HYP | ||||||
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![]() | HYDROLASE / GRIMONTIA HOLLISAE / COLLAGENASE | ||||||
Function / homology | ![]() microbial collagenase / metalloendopeptidase activity / proteolysis / zinc ion binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ueshima, S. / Yaskawa, K. / Takita, T. / Mikami, B. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Insights into the catalytic mechanism of Grimontia hollisae collagenase through structural and mutational analyses. Authors: Ueshima, S. / Yasumoto, M. / Kitagawa, Y. / Akazawa, K. / Takita, T. / Tanaka, K. / Hattori, S. / Mizutani, K. / Mikami, B. / Yasukawa, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 255.6 KB | Display | ![]() |
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PDB format | ![]() | 200.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2 MB | Display | ![]() |
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Full document | ![]() | 2 MB | Display | |
Data in XML | ![]() | 48.4 KB | Display | |
Data in CIF | ![]() | 70.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / Protein/peptide , 2 types, 8 molecules ABCEFGIJ
#1: Protein | Mass: 61901.473 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Protein/peptide | Mass: 552.578 Da / Num. of mol.: 6 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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-Non-polymers , 7 types, 678 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | ChemComp-CA / #5: Chemical | ChemComp-EDO / #6: Chemical | ChemComp-PGE / | #7: Chemical | ChemComp-PEG / | #8: Chemical | ChemComp-TRS / | #9: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.94 Å3/Da / Density % sol: 58.2 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.2M CALCIUM ACETATE, 0.1M MES SODIUM HYDROXIDE PH 6.0, 20% (W/V) POLYETHYLENE GLYCOL 8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Feb 3, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→37.44 Å / Num. obs: 96513 / % possible obs: 99.3 % / Redundancy: 3.85 % / Biso Wilson estimate: 29.85 Å2 / CC1/2: 0.898 / Rmerge(I) obs: 0.08 / Rrim(I) all: 0.093 / Net I/σ(I): 12.5 |
Reflection shell | Resolution: 2→2.12 Å / Redundancy: 3.96 % / Rmerge(I) obs: 0.662 / Mean I/σ(I) obs: 2.24 / Num. unique obs: 15381 / CC1/2: 0.761 / Rrim(I) all: 0.762 / % possible all: 98.7 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.82 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→37.44 Å
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Refine LS restraints |
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LS refinement shell |
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