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Open data
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Basic information
Entry | Database: PDB / ID: 8jsd | ||||||
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Title | Alginate lyase mutant-D180G | ||||||
![]() | Alginate lyase | ||||||
![]() | LYASE / mutation | ||||||
Function / homology | Alginate lyase 2 / Alginate lyase / hydrolase activity, hydrolyzing O-glycosyl compounds / Concanavalin A-like lectin/glucanase domain superfamily / carbohydrate metabolic process / lyase activity / Alginate lyase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, X. / Pan, L.X. / Yang, D.F. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Substrate-binding mode guided protein design of alginate lyase FlAlyA for altered end-product distribution Authors: Zhang, X. / Yang, D.F. / Pan, L.X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 177.2 KB | Display | ![]() |
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PDB format | ![]() | 136.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 418.7 KB | Display | ![]() |
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Full document | ![]() | 418.8 KB | Display | |
Data in XML | ![]() | 14 KB | Display | |
Data in CIF | ![]() | 21.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 29956.881 Da / Num. of mol.: 1 / Mutation: D180G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.1 % / Description: bulk |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: DL-Malic acid |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER2 S 9M / Detector: PIXEL / Date: May 7, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979183 Å / Relative weight: 1 |
Reflection | Resolution: 1.414→31.23 Å / Num. obs: 55650 / % possible obs: 96.11 % / Redundancy: 8.9 % / Biso Wilson estimate: 20.69 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.04724 / Rpim(I) all: 0.0159 / Rrim(I) all: 0.04989 / Net I/σ(I): 22.74 |
Reflection shell | Resolution: 1.414→1.465 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.4322 / Num. unique obs: 5177 / CC1/2: 0.914 / CC star: 0.977 / Rpim(I) all: 0.2143 / Rrim(I) all: 0.4858 / % possible all: 89.86 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Refinement step | Cycle: LAST / Resolution: 1.414→31.23 Å
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Refine LS restraints |
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