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Open data
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Basic information
Entry | Database: PDB / ID: 8jqz | ||||||
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Title | Crystal Structure of GppNHp-bound mIRGB10 | ||||||
![]() | Immunity-related GTPase family member b10 | ||||||
![]() | IMMUNE SYSTEM / GTP hydrolase | ||||||
Function / homology | ![]() defense response to other organism / cellular response to interferon-beta / GTPase activity / endoplasmic reticulum membrane / GTP binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ha, H.J. / Park, H.H. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of IRGB10 oligomerization by GTP hydrolysis. Authors: Ha, H.J. / Kim, J.H. / Lee, G.H. / Kim, S. / Park, H.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 376.3 KB | Display | ![]() |
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PDB format | ![]() | 260.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1017.3 KB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 27.9 KB | Display | |
Data in CIF | ![]() | 36.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8jqyC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 47119.605 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: Irgb10 / Production host: ![]() ![]() #2: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.59 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 20% (w/v) PEG 2,000 monomethyl ether (MME), 0.2 M trimethylamine n-oxide, 0.1 M Tris-HCl (pH 8.5) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Nov 29, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.05→29.43 Å / Num. obs: 17524 / % possible obs: 99.44 % / Redundancy: 6.6 % / Biso Wilson estimate: 84.19 Å2 / CC1/2: 0.998 / Net I/σ(I): 12.86 |
Reflection shell | Resolution: 3.05→3.159 Å / Redundancy: 6.8 % / Num. unique obs: 1743 / CC1/2: 0.866 / % possible all: 99.43 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 93.03 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.05→29.43 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 36.8027082296 Å / Origin y: -21.5252278279 Å / Origin z: 28.8807013495 Å
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Refinement TLS group | Selection details: all |