+Open data
-Basic information
Entry | Database: PDB / ID: 8jah | |||||||||
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Title | Crystal structure of human CLEC12A C-type lectin domain | |||||||||
Components | C-type lectin domain family 12 member A | |||||||||
Keywords | STRUCTURAL PROTEIN / Receptor | |||||||||
Function / homology | Function and homology information signaling receptor regulator activity / tertiary granule membrane / specific granule membrane / transmembrane signaling receptor activity / carbohydrate binding / Neutrophil degranulation / signal transduction / plasma membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.58 Å | |||||||||
Authors | Lei, Q. / Tang, H. / Dong, X. | |||||||||
Funding support | China, 2items
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Citation | Journal: J.Biol.Chem. / Year: 2024 Title: Mechanistic insights into the C-type lectin receptor CLEC12A-mediated immune recognition of monosodium urate crystal. Authors: Tang, H. / Xiao, Y. / Qian, L. / Wang, Z. / Lu, M. / Yao, N. / Zhou, T. / Tian, F. / Cao, L. / Zheng, P. / Dong, X. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8jah.cif.gz | 132.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8jah.ent.gz | 84.7 KB | Display | PDB format |
PDBx/mmJSON format | 8jah.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ja/8jah ftp://data.pdbj.org/pub/pdb/validation_reports/ja/8jah | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 14497.507 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CLEC12A, CLL1, DCAL2, MICL / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q5QGZ9 #2: Chemical | #3: Chemical | ChemComp-K / | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.26 % |
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Crystal grow | Temperature: 289.15 K / Method: vapor diffusion, sitting drop / Details: 20% (w/v) PEG 3350, 0.2 M Potassium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97853 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 23, 2019 |
Radiation | Monochromator: PILATUS XXX, S/N XX-XXX / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97853 Å / Relative weight: 1 |
Reflection | Resolution: 2.58→43.01 Å / Num. obs: 8840 / % possible obs: 92.38 % / Redundancy: 13.4 % / Biso Wilson estimate: 43.56 Å2 / Rmerge(I) obs: 0.206 / Net I/σ(I): 13.357 |
Reflection shell | Resolution: 2.58→2.67 Å / Rmerge(I) obs: 0.953 / Mean I/σ(I) obs: 1.462 / Num. unique obs: 751 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.58→43.01 Å / SU ML: 0.2701 / Cross valid method: FREE R-VALUE / σ(F): 0.23 / Phase error: 24.5232 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 44.82 Å2 | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.58→43.01 Å
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Refine LS restraints |
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LS refinement shell |
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