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- PDB-8j4a: Crystal structure of OY phytoplasma SAP05 in complex with AtRPN10 -
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Open data
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Basic information
Entry | Database: PDB / ID: 8j4a | |||||||||||||||
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Title | Crystal structure of OY phytoplasma SAP05 in complex with AtRPN10 | |||||||||||||||
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![]() | PLANT PROTEIN / ubiquitin-independent protein degradation | |||||||||||||||
Function / homology | ![]() stamen formation / post-embryonic root development / response to cytokinin / root hair elongation / regulation of seed germination / peptide receptor activity / response to misfolded protein / leaf senescence / leaf development / pollen development ...stamen formation / post-embryonic root development / response to cytokinin / root hair elongation / regulation of seed germination / peptide receptor activity / response to misfolded protein / leaf senescence / leaf development / pollen development / response to auxin / response to sucrose / response to abscisic acid / proteasome regulatory particle, base subcomplex / proteasome assembly / polyubiquitin modification-dependent protein binding / response to salt stress / proteasome complex / protein catabolic process / response to heat / proteasome-mediated ubiquitin-dependent protein catabolic process / DNA damage response / nucleus / plasma membrane / cytosol Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() ![]() | |||||||||||||||
Method | ![]() ![]() ![]() | |||||||||||||||
![]() | Dong, C. / Yan, X. / Yuan, X. | |||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular basis of SAP05-mediated ubiquitin-independent proteasomal degradation of transcription factors. Authors: Yan, X. / Yuan, X. / Lv, J. / Zhang, B. / Huang, Y. / Li, Q. / Ma, J. / Li, Y. / Wang, X. / Li, Y. / Yu, Y. / Liu, Q. / Liu, T. / Mi, W. / Dong, C. | |||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 166 KB | Display | ![]() |
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PDB format | ![]() | 105.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 447.6 KB | Display | ![]() |
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Full document | ![]() | 453.7 KB | Display | |
Data in XML | ![]() | 27.8 KB | Display | |
Data in CIF | ![]() | 41.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8j48C ![]() 8j49C ![]() 8j4bC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12288.711 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: OY-M / Gene: PAM_518 / Production host: ![]() ![]() #2: Protein | Mass: 20913.914 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.78 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2 M Lithium acetate dehydrate, 18% PEG3350, 0.1 M Guanine hydrochloride |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 23, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 1.97→48.95 Å / Num. obs: 38214 / % possible obs: 94.58 % / Redundancy: 2.8 % / Biso Wilson estimate: 5.74 Å2 / Rmerge(I) obs: 0.066 / Net I/σ(I): 11.87 |
Reflection shell | Resolution: 1.97→2.04 Å / Rmerge(I) obs: 0.4679 / Mean I/σ(I) obs: 2.85 / Num. unique obs: 3983 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.23 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.97→48.95 Å
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Refine LS restraints |
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LS refinement shell |
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