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- PDB-8j3n: Solution NMR structure of purS subunit of phosphoribosylformylgly... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8j3n | ||||||
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Title | Solution NMR structure of purS subunit of phosphoribosylformylglycinamidine synthase enzyme from Staphylococcus aureus | ||||||
![]() | Phosphoribosylformylglycinamidine synthase subunit PurS | ||||||
![]() | LYASE / Phosphoribosylformylglycinamidine synthase / subunit PurS / Staphylococcus aureus | ||||||
Function / homology | Phosphoribosylformylglycinamidine synthase subunit PurS / Phosphoribosylformylglycinamidine (FGAM) synthase / Phosphoribosylformylglycinamidine synthase subunit PurS-like superfamily / phosphoribosylformylglycinamidine synthase / phosphoribosylformylglycinamidine synthase activity / 'de novo' IMP biosynthetic process / ATP binding / cytoplasm / Phosphoribosylformylglycinamidine synthase subunit PurS![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / na | ||||||
![]() | Wahab, A. / Ashraf, F. / Choudhary, M.I. | ||||||
Funding support | 1items
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![]() | ![]() Title: Solution NMR structure of purS subunit of phosphoribosylformylglycinamidine synthase enzyme from Staphylococcus aureus Authors: Wahab, A. / Ashraf, F. / Choudhary, M.I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 567.7 KB | Display | ![]() |
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PDB format | ![]() | 480.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 404.4 KB | Display | ![]() |
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Full document | ![]() | 549.9 KB | Display | |
Data in XML | ![]() | 36.9 KB | Display | |
Data in CIF | ![]() | 55.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10060.316 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: ATCC Source: (gene. exp.) ![]() Gene: purS, SAV1067 / Production host: ![]() ![]() References: UniProt: A0A0H3JUS1, phosphoribosylformylglycinamidine synthase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 1.2 mM 15N,13C Uniformly labeled Subunit PurS of phosphoribosylformylglycinamidine synthase, 20 mM sodium phosphate, 20 mM sodium chloride, 1 mM DTT, 0.03 % sodium azide, 0.15 mM DSS, 95% H2O/5% D2O Details: 1.2 mM protein in 20 mM sodium phosphate buffer pH 6.0, 20 mM sodium chloride, 1 mM DTT. Label: 15N,13C / Solvent system: 95% H2O/5% D2O | ||||||||||||||||||||||||||||
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Sample conditions | Details: 1.2 mM protein in 20 mM sodium phosphate buffer pH 6.0, 20 mM sodium chloride, 1 mM DTT, NaN3 0.03%, DSS 0.15 mM Ionic strength: 20 mM / Label: 15N,13C Uniformly Labeled / pH: 6.0 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III HD / Manufacturer: Bruker / Model: AVANCE III HD / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: na / Software ordinal: 6 | ||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 18 |