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- PDB-8j31: Glucosyl transferase crystallized in the presence of beta carotene -
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Open data
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Basic information
Entry | Database: PDB / ID: 8j31 | ||||||
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Title | Glucosyl transferase crystallized in the presence of beta carotene | ||||||
![]() | Glycosyltransferase | ||||||
![]() | TRANSFERASE / Glucosyl transferase | ||||||
Function / homology | UDP-glycosyltransferase family, conserved site / UDP-glycosyltransferases signature. / UDP-glycosyltransferase activity / UDP-glucoronosyl and UDP-glucosyl transferase / UDP-glucuronosyl/UDP-glucosyltransferase / Transferases; Glycosyltransferases; Hexosyltransferases / Glycosyltransferase![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Arold, S.T. / Hameed, U.F.S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Glucosyl transferase crystallized in the presence of beta carotene Authors: Arold, S.T. / Hameed, U.F.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 190.5 KB | Display | ![]() |
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PDB format | ![]() | 153 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 53703.672 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.76 Å3/Da / Density % sol: 67.29 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop Details: 0.2 M Magnesium chloride hexahydrate, 0.1 M Tris pH 7.0, 10% w/v PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Mar 19, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.987 Å / Relative weight: 1 |
Reflection | Resolution: 3.78→49.51 Å / Num. obs: 8122 / % possible obs: 99.6 % / Redundancy: 17.4 % / CC1/2: 0.89 / Net I/σ(I): 3.6 |
Reflection shell | Resolution: 3.78→4.23 Å / Num. unique obs: 2240 / CC1/2: 0.64 |
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Processing
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Refinement | Method to determine structure: ![]() Details: U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : RESIDUAL ONLY
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Solvent computation | Ion probe radii: 1 Å / Shrinkage radii: 1 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 167.007 Å2
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Refinement step | Cycle: LAST / Resolution: 3.78→49.16 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.78→3.877 Å
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Refinement TLS params. | Method: refined / Origin x: -8.273 Å / Origin y: 40.206 Å / Origin z: -20.234 Å
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