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- PDB-8ius: Crystal structure of Manganese-free N(omega)-hydroxy-L-arginine h... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8ius | ||||||
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Title | Crystal structure of Manganese-free N(omega)-hydroxy-L-arginine hydrolase with reduced Cys86 | ||||||
![]() | N(omega)-hydroxy-L-arginine amidinohydrolase | ||||||
![]() | HYDROLASE | ||||||
Function / homology | ![]() Nomega-hydroxy-L-arginine amidinohydrolase / hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines / arginase activity / arginine catabolic process to ornithine / antibiotic biosynthetic process / manganese ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Oda, K. / Matoba, Y. | ||||||
Funding support | 1items
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![]() | ![]() Title: Copper inactivates DcsB by oxidation of the Cys86 to cysteine sulfinic aicd Authors: Oda, K. / Komaguchi, K. / Matoba, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 119.8 KB | Display | ![]() |
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PDB format | ![]() | 91.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.3 MB | Display | ![]() |
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Full document | ![]() | 2.3 MB | Display | |
Data in XML | ![]() | 22.7 KB | Display | |
Data in CIF | ![]() | 32.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8iutC ![]() 8iuuC ![]() 8iuvC ![]() 8iuwC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30016.701 Da / Num. of mol.: 2 / Fragment: N(omega)-hydroxy-L-arginine amidinohydrolase Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: D2Z025, Nomega-hydroxy-L-arginine amidinohydrolase #2: Chemical | ChemComp-MN / | #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 36.96 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 8.3 / Details: PEG 4000, MgCl2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 25, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.14→43.98 Å / Num. obs: 24286 / % possible obs: 97 % / Redundancy: 1.8 % / CC1/2: 0.995 / Rmerge(I) obs: 0.041 / Rpim(I) all: 0.041 / Rrim(I) all: 0.058 / Χ2: 0.23 / Net I/σ(I): 5.3 / Num. measured all: 44917 |
Reflection shell | Resolution: 2.14→2.2 Å / % possible obs: 96 % / Redundancy: 1.9 % / Rmerge(I) obs: 0.105 / Num. measured all: 3828 / Num. unique obs: 2000 / CC1/2: 0.966 / Rpim(I) all: 0.105 / Rrim(I) all: 0.149 / Χ2: 0.17 / Net I/σ(I) obs: 2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.14→35.25 Å
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Refine LS restraints |
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LS refinement shell |
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