[English] 日本語
Yorodumi- PDB-8in6: Eisenia hydrolysis-enhancing protein from Aplysia kurodai complex... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8in6 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Eisenia hydrolysis-enhancing protein from Aplysia kurodai complex with tannic acid | ||||||||||||
Components | 25 kDa polyphenol-binding protein | ||||||||||||
Keywords | UNKNOWN FUNCTION / protect glucosidase from inhibition | ||||||||||||
Function / homology | Chitin-binding domain type 2 / Chitin binding domain / Chitin binding Peritrophin-A domain / Chitin-binding type-2 domain profile. / Chitin binding domain superfamily / chitin binding / extracellular region / BETA-1,2,3,4,6-PENTA-O-GALLOYL-D-GLUCOPYRANOSE / 25 kDa polyphenol-binding protein Function and homology information | ||||||||||||
Biological species | Aplysia kurodai (Kuroda's sea hare) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||||||||
Authors | Sun, X.M. / Ye, Y.X. / Kato, K. / Yu, J. / Yao, M. | ||||||||||||
Funding support | Japan, 3items
| ||||||||||||
Citation | Journal: Elife / Year: 2023 Title: Structural basis of EHEP-mediated offense against phlorotannin-induced defense from brown algae to protect aku BGL activity. Authors: Sun, X. / Ye, Y. / Sakurai, N. / Wang, H. / Kato, K. / Yu, J. / Yuasa, K. / Tsuji, A. / Yao, M. | ||||||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 8in6.cif.gz | 119.2 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb8in6.ent.gz | 75.1 KB | Display | PDB format |
PDBx/mmJSON format | 8in6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/in/8in6 ftp://data.pdbj.org/pub/pdb/validation_reports/in/8in6 | HTTPS FTP |
---|
-Related structure data
Related structure data | 8in1C 8in3C 8in4C C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 24669.895 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Aplysia kurodai (Kuroda's sea hare) / References: UniProt: A0A1B4XTR1 |
---|---|
#2: Chemical | ChemComp-GGP / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 1.89 Å3/Da / Density % sol: 34.89 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 1.0 M sodium acetate, 0.1 M imidazole (pH 6.5) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-1A / Wavelength: 0.98 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 6, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→46.84 Å / Num. obs: 15284 / % possible obs: 99.9 % / Redundancy: 6.4 % / Biso Wilson estimate: 29.7 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.0821 / Net I/σ(I): 15.39 |
Reflection shell | Resolution: 1.9→1.968 Å / Redundancy: 6.4 % / Rmerge(I) obs: 0.8228 / Num. unique obs: 1493 / CC1/2: 0.737 / % possible all: 99.73 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.9→35.89 Å / SU ML: 0.2654 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 25.5441 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
| ||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→35.89 Å
| ||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
| ||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Origin x: -5.03160430185 Å / Origin y: 6.42106978878 Å / Origin z: -7.63147150499 Å
| ||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group | Selection details: all |