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Yorodumi- PDB-8imh: Solution structure of the N terminal domain of MazE9 antitoxin (n... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8imh | |||||||||
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Title | Solution structure of the N terminal domain of MazE9 antitoxin (nMazE9) from Mycobacterium tuberculosis | |||||||||
Components | Antitoxin MazE9 | |||||||||
Keywords | ANTITOXIN / Ribbon-helix-helix / RHH motif / DNA binding domain / transcription factor / toxin-antitoxin system / Mycobacterium tuberculosis MazEF9 system / mazE-mt1 | |||||||||
Function / homology | positive regulation of growth / Ribbon-helix-helix protein, CopG / Ribbon-helix-helix protein, copG family / toxin sequestering activity / regulation of DNA-templated transcription / Antitoxin MazE9 Function and homology information | |||||||||
Biological species | Mycobacterium tuberculosis H37Rv (bacteria) | |||||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing | |||||||||
Authors | Basu Roy, T. / Sarma, S.P. | |||||||||
Funding support | India, 2items
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Citation | Journal: Proteins / Year: 2023 Title: Insights into the solution structure and transcriptional regulation of the MazE9 antitoxin in Mycobacterium tuberculosis. Authors: Roy, T.B. / Sarma, S.P. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8imh.cif.gz | 511 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8imh.ent.gz | 428.8 KB | Display | PDB format |
PDBx/mmJSON format | 8imh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8imh_validation.pdf.gz | 548.9 KB | Display | wwPDB validaton report |
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Full document | 8imh_full_validation.pdf.gz | 808.3 KB | Display | |
Data in XML | 8imh_validation.xml.gz | 34 KB | Display | |
Data in CIF | 8imh_validation.cif.gz | 53.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/im/8imh ftp://data.pdbj.org/pub/pdb/validation_reports/im/8imh | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4624.309 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis H37Rv (bacteria) Gene: mazE9, mazE-mt1, Rv2801A / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0CL61 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 mM / Label: phosphate buffer pH 7.0 / pH: 7.0 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |