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Yorodumi- PDB-8ila: Crystal structure of LmbT from Streptomyces lincolnensis NRRL ISP... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8ila | |||||||||
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Title | Crystal structure of LmbT from Streptomyces lincolnensis NRRL ISP-5355 in complex with substrates | |||||||||
Components | Glycosyltransferase | |||||||||
Keywords | BIOSYNTHETIC PROTEIN / Glycosyltransferase | |||||||||
Function / homology | Glycosyl transferase, family 1 / Glycosyl transferases group 1 / glycosyltransferase activity / GUANOSINE-5'-DIPHOSPHATE / Chem-Q3L / LmbT Function and homology information | |||||||||
Biological species | Streptomyces lincolnensis (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.79 Å | |||||||||
Authors | Dai, Y. / Qiao, H. / Xia, M. / Fang, P. / Liu, W. | |||||||||
Funding support | China, 2items
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Citation | Journal: Acs Chem.Biol. / Year: 2023 Title: Structural Basis of Low-Molecular-Weight Thiol Glycosylation in Lincomycin A Biosynthesis. Authors: Dai, Y. / Cheng, Y. / Ding, W. / Qiao, H. / Zhang, D. / Zhong, G. / Xia, M. / Tao, J. / Sun, P. / Fang, P. / Liu, W. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8ila.cif.gz | 647.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8ila.ent.gz | 537.8 KB | Display | PDB format |
PDBx/mmJSON format | 8ila.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8ila_validation.pdf.gz | 2.6 MB | Display | wwPDB validaton report |
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Full document | 8ila_full_validation.pdf.gz | 2.7 MB | Display | |
Data in XML | 8ila_validation.xml.gz | 64.8 KB | Display | |
Data in CIF | 8ila_validation.cif.gz | 86 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/il/8ila ftp://data.pdbj.org/pub/pdb/validation_reports/il/8ila | HTTPS FTP |
-Related structure data
Related structure data | 8il0C C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 49408.723 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces lincolnensis (bacteria) / Gene: GJU35_01490 / Production host: Escherichia coli (E. coli) / References: UniProt: A9Y8T1 #2: Chemical | ChemComp-GDP / #3: Chemical | ChemComp-Q3L / ( #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.12 Å3/Da / Density % sol: 60.55 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 8% PEG 20000, 0.05 M Tris pH 8.5, 1.2 M Sodium formate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL10U2 / Wavelength: 0.979183 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 8, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979183 Å / Relative weight: 1 |
Reflection | Resolution: 2.79→100.22 Å / Num. obs: 62181 / % possible obs: 99.7 % / Redundancy: 7.1 % / CC1/2: 0.998 / Rmerge(I) obs: 0.091 / Net I/σ(I): 16.2 |
Reflection shell | Resolution: 2.79→2.94 Å / Num. unique obs: 9020 / CC1/2: 0.828 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.79→68.87 Å / SU ML: 0.39 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 30.92 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.79→68.87 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 39.3314 Å / Origin y: 20.9171 Å / Origin z: 31.341 Å
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Refinement TLS group | Selection details: all |