+Open data
-Basic information
Entry | Database: PDB / ID: 8ikb | ||||||
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Title | Cryo-EM structure of hnRAC1-2I fibril. | ||||||
Components | GLY-PHI-GLY-GLY-ASN-ASP-ASN-PHE-GLY | ||||||
Keywords | PROTEIN FIBRIL / reversible fibril | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.71 Å | ||||||
Authors | Li, D.N. / Ma, Y.Y. / Li, D. / Dai, B. / Liu, C. | ||||||
Funding support | 1items
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Citation | Journal: To Be Published Title: Cryo-EM structure of hnRAC1-2I fibril. Authors: Li, D.N. / Ma, Y.Y. / Li, D. / Dai, B. / Liu, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8ikb.cif.gz | 45.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8ikb.ent.gz | 37.7 KB | Display | PDB format |
PDBx/mmJSON format | 8ikb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8ikb_validation.pdf.gz | 1.3 MB | Display | wwPDB validaton report |
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Full document | 8ikb_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 8ikb_validation.xml.gz | 32.6 KB | Display | |
Data in CIF | 8ikb_validation.cif.gz | 44.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ik/8ikb ftp://data.pdbj.org/pub/pdb/validation_reports/ik/8ikb | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein/peptide | Mass: 1009.760 Da / Num. of mol.: 28 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
-Sample preparation
Component | Name: Cryo-EM structure of hnRAC1-2I fibril. / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
CTF correction | Type: NONE |
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Helical symmerty | Angular rotation/subunit: 179.55 ° / Axial rise/subunit: 2.4 Å / Axial symmetry: C1 |
3D reconstruction | Resolution: 3.71 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 6826 / Symmetry type: HELICAL |