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Open data
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Basic information
Entry | Database: PDB / ID: 8ijt | ||||||
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Title | crystal structure of Hyp N135A mutant from Hypoxylon sp. E7406B | ||||||
![]() | Terpene synthase | ||||||
![]() | TRANSFERASE / Terpene synthetase / UNKNOWN FUNCTION | ||||||
Function / homology | Terpene synthase family 2, C-terminal metal binding / Isoprenoid synthase domain superfamily / Terpene synthase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gao, J. / Su, L.Q. / Li, Q. / Han, X. / Wei, H.L. / Dai, Z.J. / Liu, W.D. | ||||||
Funding support | ![]()
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![]() | ![]() Title: crystal structure of Hyp N135A mutant from Hypoxylon sp. E7406B Authors: Gao, J. / Su, L.Q. / Li, Q. / Han, X. / Wei, H.L. / Dai, Z.J. / Liu, W.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 261.9 KB | Display | ![]() |
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PDB format | ![]() | 210.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 466.6 KB | Display | ![]() |
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Full document | ![]() | 493.8 KB | Display | |
Data in XML | ![]() | 45.1 KB | Display | |
Data in CIF | ![]() | 62.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 41666.305 Da / Num. of mol.: 4 / Mutation: N135A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.99 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop Details: 13% PEG4000, 0.1 M Na3Cit, pH5.6, 0.15 M NaCl, 0.01 M MgCl2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Sep 2, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.558→58.437 Å / Num. obs: 48170 / % possible obs: 97.7 % / Redundancy: 3.5 % / Biso Wilson estimate: 51.96 Å2 / CC1/2: 0.998 / Net I/σ(I): 7.8 |
Reflection shell | Resolution: 2.558→2.602 Å / Num. unique obs: 2372 / CC1/2: 0.87 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: AlphaFold Resolution: 2.56→31.37 Å / SU ML: 0.33 / Cross valid method: FREE R-VALUE / σ(F): 1.24 / Phase error: 29.61 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.56→31.37 Å
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Refine LS restraints |
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LS refinement shell |
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