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- PDB-8ij6: Crystal structure of alcohol dehydrogenase from Burkholderia glad... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8ij6 | ||||||
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Title | Crystal structure of alcohol dehydrogenase from Burkholderia gladioli with NADP | ||||||
![]() | Putative short-chain dehydrogenases/reductase family protein | ||||||
![]() | OXIDOREDUCTASE / alcohol dehydrogenase | ||||||
Function / homology | oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor / PKS_KR / Enoyl-(Acyl carrier protein) reductase / Short-chain dehydrogenase/reductase SDR / NAD(P)-binding domain superfamily / NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Putative short-chain dehydrogenases/reductase family protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Han, X. / Mei, Z.L. / Liu, W.D. / Sun, Z.T. / Ma, J.A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of alcohol dehydrogenase from Burkholderia gladioli with NADP Authors: Han, X. / Mei, Z.L. / Liu, W.D. / Sun, Z.T. / Ma, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 193.6 KB | Display | ![]() |
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PDB format | ![]() | 156.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.7 MB | Display | ![]() |
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Full document | ![]() | 1.7 MB | Display | |
Data in XML | ![]() | 38.8 KB | Display | |
Data in CIF | ![]() | 53.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8ijgC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25881.197 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: bgla_2g00620 / Production host: ![]() ![]() #2: Chemical | ChemComp-NAP / #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.05 Å3/Da / Density % sol: 59.7 % |
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Crystal grow | Temperature: 300 K / Method: vapor diffusion, sitting drop Details: 18% MPD, 18% PEG1000, 18% PEG3350, 0.1M HEPES, pH7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 1, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.28→53.43 Å / Num. obs: 56690 / % possible obs: 98.02 % / Redundancy: 104 % / CC1/2: 0.997 / Net I/σ(I): 1.34 |
Reflection shell | Resolution: 2.28→2.41 Å / Num. unique obs: 56690 / CC1/2: 0.773 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.28→53.42 Å
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Refine LS restraints |
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LS refinement shell |
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