+Open data
-Basic information
Entry | Database: PDB / ID: 8if0 | ||||||
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Title | NmeHNH-AcrIIC1 complex | ||||||
Components |
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Keywords | STRUCTURAL PROTEIN / complex | ||||||
Function / homology | Function and homology information maintenance of CRISPR repeat elements / endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / DNA binding / RNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | Neisseria meningitidis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.57 Å | ||||||
Authors | Zhu, Y.L. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Inhibition Mechanism of CRISPR-Cas9 by Anti-CRISPR Protein AcrIIC1 Authors: Zhu, Y.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8if0.cif.gz | 119.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8if0.ent.gz | 87.8 KB | Display | PDB format |
PDBx/mmJSON format | 8if0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/if/8if0 ftp://data.pdbj.org/pub/pdb/validation_reports/if/8if0 | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 19160.656 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Neisseria meningitidis (bacteria) / Gene: cas9 / Production host: Escherichia coli (E. coli) / References: UniProt: X5EPV9 |
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#2: Protein | Mass: 9637.853 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Neisseria meningitidis (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: A0A2D0TCG3 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43.15 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.2 M Ammonium acetate, 0.1 M MES, pH 6.2, 26% PEG3350 |
-Data collection
Diffraction | Mean temperature: 193 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.9785 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jul 18, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9785 Å / Relative weight: 1 |
Reflection | Resolution: 1.57→50 Å / Num. obs: 34393 / % possible obs: 99.7 % / Redundancy: 6.2 % / Rmerge(I) obs: 0.092 / Net I/σ(I): 25.3 |
Reflection shell | Resolution: 1.57→1.61 Å / Rmerge(I) obs: 0.092 / Num. unique obs: 34393 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 1.57→50 Å / Cross valid method: FREE R-VALUE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Displacement parameters | Biso mean: 40.82 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.57→50 Å
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Refine LS restraints |
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