+Open data
-Basic information
Entry | Database: PDB / ID: 8i7h | ||||||
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Title | Meso-Diaminopimelate dehydrogenase | ||||||
Components | Meso-diaminopimelate D-dehydrogenase | ||||||
Keywords | OXIDOREDUCTASE / Meso-Diaminopimelate dehydrogenase / oxidordeuctase / D-Amino acid / Reductive amination | ||||||
Function / homology | 2,6-DIAMINOPIMELIC ACID / : Function and homology information | ||||||
Biological species | Proteus vulgaris (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 3.02 Å | ||||||
Authors | Wu, T.F. / Song, W. | ||||||
Funding support | 1items
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Citation | Journal: To Be Published Title: Analysis of the catalytic mechanism of meso-DAPDH and extension of D-aromatic amino acid substrate scope Authors: Wu, T.F. / Song, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8i7h.cif.gz | 231.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8i7h.ent.gz | 187.8 KB | Display | PDB format |
PDBx/mmJSON format | 8i7h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8i7h_validation.pdf.gz | 2.1 MB | Display | wwPDB validaton report |
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Full document | 8i7h_full_validation.pdf.gz | 2.1 MB | Display | |
Data in XML | 8i7h_validation.xml.gz | 42.3 KB | Display | |
Data in CIF | 8i7h_validation.cif.gz | 56.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i7/8i7h ftp://data.pdbj.org/pub/pdb/validation_reports/i7/8i7h | HTTPS FTP |
-Related structure data
Related structure data | 8i78C C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
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-Components
#1: Protein | Mass: 32274.896 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Proteus vulgaris (bacteria) / Gene: EKQ45_05540 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A857SCE6 #2: Chemical | ChemComp-API / #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.17 Å3/Da / Density % sol: 70.49 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion Details: 0.1M Sodium acetate trihydrate,2M ammonium sulfate, pH 4.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SEALED TUBE / Type: BRUKER D8 QUEST / Wavelength: 1.541 Å |
Detector | Type: Bruker PHOTON II / Detector: PIXEL / Date: Feb 20, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.541 Å / Relative weight: 1 |
Reflection | Resolution: 3.02→47.29 Å / Num. obs: 40836 / % possible obs: 97 % / Redundancy: 5.7 % / CC1/2: 0.98 / Net I/σ(I): 8 |
Reflection shell | Resolution: 3.02→3.14 Å / Redundancy: 4.4 % / Num. unique obs: 4541 / CC1/2: 0.701 / % possible all: 96.4 |
-Processing
Software | Name: PHENIX / Version: V1.0 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.02→47 Å / Cor.coef. Fo:Fc: 0.919 / Cor.coef. Fo:Fc free: 0.879 / SU B: 15.904 / SU ML: 0.273 / Cross valid method: THROUGHOUT / ESU R: 1.2 / ESU R Free: 0.367 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.564 Å2
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Refinement step | Cycle: 1 / Resolution: 3.02→47 Å
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