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Open data
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Basic information
Entry | Database: PDB / ID: 8i33 | ||||||
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Title | Coil 1a of lamin A (residue 25-65) | ||||||
![]() | Prelamin-A/C | ||||||
![]() | STRUCTURAL PROTEIN / Lamin A / coil 1a / coiled-coil | ||||||
Function / homology | ![]() nuclear lamina / intermediate filament / nuclear envelope / nuclear speck Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jeong, S. / Ha, N.C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Coil 1a of lamin A (residue 25-65) Authors: Jeong, S. / Ha, N.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 55.5 KB | Display | ![]() |
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PDB format | ![]() | 33.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 420.9 KB | Display | ![]() |
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Full document | ![]() | 421.2 KB | Display | |
Data in XML | ![]() | 8.5 KB | Display | |
Data in CIF | ![]() | 11.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein/peptide | Mass: 4958.617 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) ![]() #2: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.43 % |
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Crystal grow | Temperature: 287 K / Method: vapor diffusion, hanging drop / Details: NaCl, PEG 6000, sodium thiocyanate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Oct 20, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.62→50 Å / Num. obs: 136711 / % possible obs: 96.6 % / Redundancy: 3.6 % / Biso Wilson estimate: 13.06 Å2 / CC1/2: 0.993 / Net I/σ(I): 24.29 |
Reflection shell | Resolution: 1.62→1.65 Å / Redundancy: 3.1 % / Mean I/σ(I) obs: 6.74 / Num. unique obs: 18503 / CC1/2: 0.977 / Rpim(I) all: 0.108 / % possible all: 91.8 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.23 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.62→33.31 Å
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Refine LS restraints |
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LS refinement shell |
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