Mass: 13716.472 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Toxoplasma gondii GT1 (eukaryote) / Gene: TGGT1_207690 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A125YJ71
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
anisotropic
3
3D HN(CA)CB
1
2
1
anisotropic
3
3DCBCA(CO)NH
1
3
1
anisotropic
3
3D HNCA
1
4
1
anisotropic
3
3D HNCO
1
5
1
anisotropic
3
2D 1H-15N HSQC
1
6
1
anisotropic
3
3D 1H-13C NOESY
1
7
1
anisotropic
3
3D 1H-15N NOESY
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Sample preparation
Details
Type: solution Contents: 0.5 mM [U-100% 13C; U-100% 15N] Protein, 25 mM sodium phosphate, 100 mM sodium chloride, 10 mM sodium azide, 1 mM PMSF, 90% H2O/10% D2O Details: The purified protein was concentrated to 0.1-0.5 mM in buffer 25 mM sodium phosphate, 100 mM NaCl, 10 mM sodium azide and 1mM PMSF with pH 4.5 for NMR. Label: 15N_sample, 13C_sample / Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.5mM
Protein
[U-100% 13C; U-100% 15N]
1
25mM
sodiumphosphate
naturalabundance
1
100mM
sodiumchloride
naturalabundance
1
10mM
sodiumazide
naturalabundance
1
1mM
PMSF
naturalabundance
1
Sample conditions
Details: The purified protein was concentrated to 0.1-0.5 mM in buffer 25 mM sodium phosphate, 100 mM NaCl, 10 mM sodium azide and 1mM PMSF with pH 4.5 for NMR. Ionic strength: 125 mM / Label: cis form / pH: 4.5 / Pressure: 1 atm / Temperature: 310 K
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NMR measurement
NMR spectrometer
Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 850 MHz
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Processing
NMR software
Name
Developer
Classification
CYANA
Guntert, MumenthalerandWuthrich
refinement
CYANA
Guntert, MumenthalerandWuthrich
structurecalculation
Sparky
Goddard
chemicalshiftassignment
Sparky
Goddard
peakpicking
Refinement
Method: distance geometry / Software ordinal: 1
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100000 / Conformers submitted total number: 20
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