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Open data
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Basic information
Entry | Database: PDB / ID: 8hpi | ||||||
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Title | Crystal structure of Tyrosinase from Priestia megaterium | ||||||
![]() | Tyrosinase | ||||||
![]() | OXIDOREDUCTASE / Tyrosinase Priestia megaterium | ||||||
Function / homology | Tyrosinase CuA-binding region signature. / Common central domain of tyrosinase / Tyrosinase and hemocyanins CuB-binding region signature. / Tyrosinase copper-binding domain / Di-copper centre-containing domain superfamily / oxidoreductase activity / Tyrosinase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Huang, Y. / Song, W. | ||||||
Funding support | 1items
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![]() | ![]() Title: Crystal structure of Tyrosinase from Priestia megaterium Authors: Huang, Y. / Song, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 133 KB | Display | ![]() |
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PDB format | ![]() | 101.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446 KB | Display | ![]() |
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Full document | ![]() | 447.7 KB | Display | |
Data in XML | ![]() | 23.6 KB | Display | |
Data in CIF | ![]() | 32.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33372.316 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: melC1 Production host: ![]() ![]() References: UniProt: A0A8D3X086 #2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-EDO / #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.85 Å3/Da / Density % sol: 56.92 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion Details: PEG 4000, Zinc acetate dehydrate, Sodium acetate trihydrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SEALED TUBE / Type: BRUKER D8 QUEST / Wavelength: 1.54184 Å |
Detector | Type: Bruker PHOTON II / Detector: PIXEL / Date: May 3, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54184 Å / Relative weight: 1 |
Reflection | Resolution: 2.54→46.94 Å / Num. obs: 39392 / % possible obs: 99.4 % / Redundancy: 4.5 % / CC1/2: 0.993 / Net I/σ(I): 4.08 |
Reflection shell | Resolution: 2.54→2.65 Å / Num. unique obs: 3 / CC1/2: 0.584 |
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Processing
Software | Name: Aimless / Classification: data scaling | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.934 Å2
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Refinement step | Cycle: 1 / Resolution: 2.54→46.98 Å
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