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Open data
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Basic information
Entry | Database: PDB / ID: 8ho6 | |||||||||
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Title | ScRIPK WT | |||||||||
![]() | ScRIPK kinase protein | |||||||||
![]() | PLANT PROTEIN / Kinase / RLCK VII | |||||||||
Function / homology | ADENOSINE-5'-TRIPHOSPHATE![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Fang, J.L. / Zhang, M.Q. | |||||||||
Funding support | 2items
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![]() | ![]() Title: Receptor-like cytoplasmic kinase ScRIPK in sugarcane regulates disease resistance and drought tolerance in Arabidopsis. Authors: Fang, J. / Chai, Z. / Huang, R. / Huang, C. / Ming, Z. / Chen, B. / Yao, W. / Zhang, M. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 75.2 KB | Display | ![]() |
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PDB format | ![]() | 52.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 8hoaC ![]() 8hodC ![]() 5tosS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 36416.234 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
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#2: Chemical | ChemComp-ATP / |
#3: Chemical | ChemComp-MG / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.75 Å3/Da / Density % sol: 55.2 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop Details: 0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 400, 0.19 mM Cymal-7, 10% v/v Polyethylene glycol 3350, 0.18 mM GDN |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Jul 10, 2021 |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→32.06 Å / Num. obs: 23842 / % possible obs: 99.7 % / Redundancy: 12.79 % / CC1/2: 0.998 / Rmerge(I) obs: 0.138 / Χ2: 1.02 / Net I/σ(I): 13 |
Reflection shell | Resolution: 2.1→2.15 Å / Mean I/σ(I) obs: 1.7 / Num. unique obs: 23842 / CC1/2: 0.853 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5TOS Resolution: 2.1→26.738 Å / SU ML: 0.23 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 24.48 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→26.738 Å
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Refine LS restraints |
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LS refinement shell |
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