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Open data
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Basic information
Entry | Database: PDB / ID: 8hne | ||||||
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Title | Crystal structure of the ancestral GH19 chitinase Anc4 | ||||||
![]() | Anc4, ancestral GH19 chitinase | ||||||
![]() | HYDROLASE / GH19 Chitinase | ||||||
Function / homology | Chem-1PG![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kozome, D. / Laurino, P. | ||||||
Funding support | 1items
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![]() | ![]() Title: Emergence of antifungal activity in GH19 chitinases through gain-of-function in a remote loop. Authors: Kozome, D. / Laurino, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.1 KB | Display | ![]() |
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PDB format | ![]() | 71.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 6.7 KB | Display | |
Data in CIF | ![]() | 10.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8hnfC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 24048.719 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() | ||||||
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#2: Chemical | #3: Chemical | ChemComp-1PG / | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.01 Å3/Da / Density % sol: 38.82 % |
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Crystal grow | Temperature: 316 K / Method: vapor diffusion, hanging drop Details: 15% (w/v) polyethylene glycol monomethyl ether 2000, 0.1M MES monohydrate pH 6.0 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 14, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.13→39.47 Å / Num. obs: 73261 / % possible obs: 99.62 % / Redundancy: 2 % / Biso Wilson estimate: 8.18 Å2 / CC1/2: 0.899 / Rmerge(I) obs: 0.172 / Rpim(I) all: 0.172 / Rrim(I) all: 0.2433 / Net I/σ(I): 4.14 |
Reflection shell | Resolution: 1.13→1.17 Å / Rmerge(I) obs: 0.2298 / Num. unique obs: 6976 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 9.88 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.13→39.47 Å
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Refine LS restraints |
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LS refinement shell |
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