+Open data
-Basic information
Entry | Database: PDB / ID: 8hhg | ||||||
---|---|---|---|---|---|---|---|
Title | The bacterial divisome protein complex FtsB-FtsL-FtsQ | ||||||
Components |
| ||||||
Keywords | MEMBRANE PROTEIN / Bacterial cell division / divisome / FtsB / FtsL / FtsQ / FtsBLQ / FtsQLB / membrane protein complex / heterotrimer | ||||||
Function / homology | Function and homology information cell septum assembly / cell septum / FtsZ-dependent cytokinesis / cell division site / plasma membrane Similarity search - Function | ||||||
Biological species | Escherichia coli K-12 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | ||||||
Authors | Nguyen, V.H.T. / Chen, X. | ||||||
Funding support | Taiwan, 1items
| ||||||
Citation | Journal: Nat Commun / Year: 2023 Title: Structure of the heterotrimeric membrane protein complex FtsB-FtsL-FtsQ of the bacterial divisome. Authors: Nguyen, H.T.V. / Chen, X. / Parada, C. / Luo, A.C. / Shih, O. / Jeng, U.S. / Huang, C.Y. / Shih, Y.L. / Ma, C. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 8hhg.cif.gz | 183.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb8hhg.ent.gz | 144.4 KB | Display | PDB format |
PDBx/mmJSON format | 8hhg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hh/8hhg ftp://data.pdbj.org/pub/pdb/validation_reports/hh/8hhg | HTTPS FTP |
---|
-Related structure data
Related structure data | 8hhfSC 8hhhC S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 31467.660 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K-12 / Gene: ftsQ / Production host: Escherichia coli (E. coli) / References: UniProt: J7Q602 |
---|---|
#2: Protein | Mass: 13455.919 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K-12 / Gene: ftsB / Production host: Escherichia coli (E. coli) / References: UniProt: Q1JQN6 |
#3: Protein | Mass: 13646.827 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K-12 / Gene: ftsL / Production host: Escherichia coli (E. coli) / References: UniProt: D6II44 |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 4.24 Å3/Da / Density % sol: 71.02 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8 / Details: 0.1M Tris pH 8.0, 20-35% PEG400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: SYNCHROTRON / Site: NSRRC / Beamline: TPS 05A / Wavelength: 1 Å | ||||||||||||||||||||||||
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Aug 28, 2020 | ||||||||||||||||||||||||
Radiation | Monochromator: liquid-nitrogen-cooling Si double-crystal monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||||||
Reflection | Resolution: 3.1→88.5 Å / Num. obs: 17628 / % possible obs: 99.8 % / Redundancy: 7.4 % / CC1/2: 0.996 / Rmerge(I) obs: 0.17 / Net I/σ(I): 6.9 / Num. measured all: 131166 / Scaling rejects: 354 | ||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
|
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 8HHF Resolution: 3.1→34.452 Å / SU ML: 0.45 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 33.4 / Stereochemistry target values: ML
| ||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 216.55 Å2 / Biso mean: 109.3532 Å2 / Biso min: 30 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.1→34.452 Å
| ||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
| ||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Origin x: -17.8763 Å / Origin y: -4.0685 Å / Origin z: -15.8832 Å
| ||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group |
|