+
Open data
-
Basic information
Entry | Database: PDB / ID: 8h45 | ||||||
---|---|---|---|---|---|---|---|
Title | Blasnase-T13A/P55N | ||||||
![]() | L-asparaginase | ||||||
![]() | HYDROLASE / Mutant | ||||||
Function / homology | ![]() asparagine metabolic process / asparaginase activity / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lu, F. / Wang, W. / Chi, H. / Ran, T. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Structure-based rational design of Bacillus licheniformis L-asparaginase with low/no D-asparaginase activity for a safer enzyme Authors: Lu, F. / Wang, W. / Chi, H. / Ran, T. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 146.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 111.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 8h44C ![]() 8h46C ![]() 8h47C ![]() 8h48C ![]() 8h49C ![]() 8h4aC ![]() 8h4bC ![]() 8h4cC ![]() 8h4dC ![]() 8h4eC ![]() 8h4fC ![]() 8h4gC ![]() 7c8xS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
Unit cell |
| ||||||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 36395.188 Da / Num. of mol.: 2 / Mutation: T13A, P55N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-FMT / #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.4 Å3/Da / Density % sol: 63.79 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / Details: sodium formate |
-Data collection
Diffraction | Mean temperature: 173 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 29, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 1.97→19.95 Å / Num. obs: 71787 / % possible obs: 99.9 % / Redundancy: 25.6 % / CC1/2: 0.999 / Rpim(I) all: 0.033 / Net I/σ(I): 17.1 |
Reflection shell | Resolution: 1.97→2.01 Å / Num. unique obs: 4561 / CC1/2: 0.431 / Rpim(I) all: 0.636 |
-
Processing
Software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 7C8X Resolution: 1.97→19.83 Å / Cross valid method: FREE R-VALUE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
| ||||||||||||||||||||||||
Displacement parameters | Biso mean: 42.39 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.97→19.83 Å
| ||||||||||||||||||||||||
Refine LS restraints |
|