+Open data
-Basic information
Entry | Database: PDB / ID: 8gbc | |||||||||
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Title | Homo sapiens Zalpha mutant - N173S | |||||||||
Components | Double-stranded RNA-specific adenosine deaminase | |||||||||
Keywords | RNA BINDING PROTEIN | |||||||||
Function / homology | Function and homology information somatic diversification of immune receptors via somatic mutation / negative regulation of post-transcriptional gene silencing by regulatory ncRNA / C6 deamination of adenosine / Formation of editosomes by ADAR proteins / double-stranded RNA adenine deaminase / tRNA-specific adenosine deaminase activity / supraspliceosomal complex / double-stranded RNA adenosine deaminase activity / negative regulation of protein kinase activity by regulation of protein phosphorylation / base conversion or substitution editing ...somatic diversification of immune receptors via somatic mutation / negative regulation of post-transcriptional gene silencing by regulatory ncRNA / C6 deamination of adenosine / Formation of editosomes by ADAR proteins / double-stranded RNA adenine deaminase / tRNA-specific adenosine deaminase activity / supraspliceosomal complex / double-stranded RNA adenosine deaminase activity / negative regulation of protein kinase activity by regulation of protein phosphorylation / base conversion or substitution editing / hematopoietic stem cell homeostasis / response to interferon-alpha / adenosine to inosine editing / RISC complex assembly / pre-miRNA processing / negative regulation of hepatocyte apoptotic process / definitive hemopoiesis / negative regulation of type I interferon-mediated signaling pathway / hepatocyte apoptotic process / positive regulation of viral genome replication / RNA processing / hematopoietic progenitor cell differentiation / protein export from nucleus / erythrocyte differentiation / response to virus / PKR-mediated signaling / mRNA processing / cellular response to virus / osteoblast differentiation / protein import into nucleus / double-stranded RNA binding / Interferon alpha/beta signaling / defense response to virus / innate immune response / nucleolus / DNA binding / RNA binding / nucleoplasm / membrane / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | SOLUTION NMR / na | |||||||||
Authors | Langeberg, C.J. / Nichols, P.J. / Henen, M. / Vicens, Q. / Vogeli, B. | |||||||||
Funding support | United States, 2items
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Citation | Journal: J.Mol.Biol. / Year: 2023 Title: Differential Structural Features of Two Mutant ADAR1p150 Z alpha Domains Associated with Aicardi-Goutieres Syndrome. Authors: Langeberg, C.J. / Nichols, P.J. / Henen, M.A. / Vicens, Q. / Vogeli, B. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8gbc.cif.gz | 458.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8gbc.ent.gz | 384.6 KB | Display | PDB format |
PDBx/mmJSON format | 8gbc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8gbc_validation.pdf.gz | 408.1 KB | Display | wwPDB validaton report |
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Full document | 8gbc_full_validation.pdf.gz | 579.1 KB | Display | |
Data in XML | 8gbc_validation.xml.gz | 30 KB | Display | |
Data in CIF | 8gbc_validation.cif.gz | 44.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gb/8gbc ftp://data.pdbj.org/pub/pdb/validation_reports/gb/8gbc | HTTPS FTP |
-Related structure data
Related structure data | 8gbdC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9234.648 Da / Num. of mol.: 1 / Mutation: N173S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ADAR, ADAR1, DSRAD, G1P1, IFI4 / Production host: Escherichia coli (E. coli) References: UniProt: P55265, double-stranded RNA adenine deaminase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution / Contents: 2 mM [U-13C; U-15N] protein, 95% H2O/5% D2O / Label: 15N_13C_sample / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 2 mM / Component: protein / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 100 mM / Label: conditions_1 / pH: 6.4 / Pressure: 1 atm / Temperature: 277 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 900 MHz |
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-Processing
NMR software |
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Refinement | Method: na / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |