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- PDB-8g66: Structure with SJ3149 -

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Basic information

Entry
Database: PDB / ID: 8g66
TitleStructure with SJ3149
Components
  • Casein kinase I isoform alpha
  • DNA damage-binding protein 1
  • Protein cereblon
KeywordsLIGASE / molecular glue
Function / homology
Function and homology information


negative regulation of monoatomic ion transmembrane transport / Activation of SMO / intermediate filament cytoskeleton organization / negative regulation of NLRP3 inflammasome complex assembly / cellular response to nutrient / beta-catenin destruction complex / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / positive regulation by virus of viral protein levels in host cell ...negative regulation of monoatomic ion transmembrane transport / Activation of SMO / intermediate filament cytoskeleton organization / negative regulation of NLRP3 inflammasome complex assembly / cellular response to nutrient / beta-catenin destruction complex / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / positive regulation by virus of viral protein levels in host cell / spindle assembly involved in female meiosis / Beta-catenin phosphorylation cascade / Signaling by GSK3beta mutants / CTNNB1 S33 mutants aren't phosphorylated / CTNNB1 S37 mutants aren't phosphorylated / CTNNB1 S45 mutants aren't phosphorylated / CTNNB1 T41 mutants aren't phosphorylated / epigenetic programming in the zygotic pronuclei / UV-damage excision repair / Disassembly of the destruction complex and recruitment of AXIN to the membrane / biological process involved in interaction with symbiont / Maturation of nucleoprotein / regulation of mitotic cell cycle phase transition / WD40-repeat domain binding / Cul4A-RING E3 ubiquitin ligase complex / Cul4-RING E3 ubiquitin ligase complex / Cul4B-RING E3 ubiquitin ligase complex / ubiquitin ligase complex scaffold activity / positive regulation of Rho protein signal transduction / Golgi organization / negative regulation of reproductive process / negative regulation of developmental process / locomotory exploration behavior / cullin family protein binding / viral release from host cell / positive regulation of Wnt signaling pathway / ectopic germ cell programmed cell death / negative regulation of protein-containing complex assembly / positive regulation of viral genome replication / proteasomal protein catabolic process / positive regulation of gluconeogenesis / positive regulation of TORC1 signaling / Recognition of DNA damage by PCNA-containing replication complex / DNA Damage Recognition in GG-NER / Degradation of GLI2 by the proteasome / GLI3 is processed to GLI3R by the proteasome / nucleotide-excision repair / Degradation of beta-catenin by the destruction complex / positive regulation of protein-containing complex assembly / Dual Incision in GG-NER / Transcription-Coupled Nucleotide Excision Repair (TC-NER) / Formation of TC-NER Pre-Incision Complex / negative regulation of canonical Wnt signaling pathway / Formation of Incision Complex in GG-NER / regulation of circadian rhythm / kinetochore / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / spindle / Wnt signaling pathway / positive regulation of protein catabolic process / cellular response to UV / rhythmic process / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / site of double-strand break / Neddylation / ubiquitin-dependent protein catabolic process / protein-macromolecule adaptor activity / damaged DNA binding / proteasome-mediated ubiquitin-dependent protein catabolic process / Potential therapeutics for SARS / transmembrane transporter binding / chromosome, telomeric region / cell surface receptor signaling pathway / protein phosphorylation / non-specific serine/threonine protein kinase / protein ubiquitination / protein kinase activity / viral protein processing / nuclear speck / ciliary basal body / cilium / cell division / protein serine kinase activity / DNA repair / protein serine/threonine kinase activity / apoptotic process / centrosome / DNA damage response / negative regulation of apoptotic process / protein-containing complex binding / nucleolus / perinuclear region of cytoplasm / signal transduction / protein-containing complex / extracellular space / DNA binding / extracellular exosome / nucleoplasm / ATP binding
Similarity search - Function
Yippee/Mis18/Cereblon / Yippee zinc-binding/DNA-binding /Mis18, centromere assembly / : / CULT domain / CULT domain profile. / Lon N-terminal domain profile. / Lon protease, N-terminal domain / Lon protease, N-terminal domain superfamily / ATP-dependent protease La (LON) substrate-binding domain / Found in ATP-dependent protease La (LON) ...Yippee/Mis18/Cereblon / Yippee zinc-binding/DNA-binding /Mis18, centromere assembly / : / CULT domain / CULT domain profile. / Lon N-terminal domain profile. / Lon protease, N-terminal domain / Lon protease, N-terminal domain superfamily / ATP-dependent protease La (LON) substrate-binding domain / Found in ATP-dependent protease La (LON) / RSE1/DDB1/CPSF1 second beta-propeller / Cleavage/polyadenylation specificity factor, A subunit, C-terminal / Cleavage/polyadenylation specificity factor, A subunit, N-terminal / : / CPSF A subunit region / RSE1/DDB1/CPSF1 first beta-propeller / PUA-like superfamily / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / WD40-repeat-containing domain superfamily / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / WD40/YVTN repeat-like-containing domain superfamily / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Chem-YOT / Casein kinase I isoform alpha / DNA damage-binding protein 1 / Protein cereblon
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.45 Å
AuthorsMiller, D.J. / Young, S.M. / Fischer, M.
Funding support United States, 1items
OrganizationGrant numberCountry
Alexs Lemonade Stand Foundation for Cancer United States
CitationJournal: To Be Published
Title: Structure of ternary complex with molecular glue targeting CK1A for degradation by the CRL4CRBN ubiquitin ligase
Authors: Miller, D.J. / Young, S.M. / Fischer, M.
History
DepositionFeb 14, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 13, 2023Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: DNA damage-binding protein 1
B: Protein cereblon
C: Casein kinase I isoform alpha
D: DNA damage-binding protein 1
E: Protein cereblon
F: Casein kinase I isoform alpha
hetero molecules


Theoretical massNumber of molelcules
Total (without water)369,1329
Polymers368,6256
Non-polymers5073
Water00
1
A: DNA damage-binding protein 1
B: Protein cereblon
C: Casein kinase I isoform alpha
hetero molecules


Theoretical massNumber of molelcules
Total (without water)184,7545
Polymers184,3123
Non-polymers4422
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: DNA damage-binding protein 1
E: Protein cereblon
F: Casein kinase I isoform alpha
hetero molecules


Theoretical massNumber of molelcules
Total (without water)184,3784
Polymers184,3123
Non-polymers651
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)86.660, 109.710, 112.340
Angle α, β, γ (deg.)105.490, 94.410, 100.030
Int Tables number1
Space group name H-MP1
Space group name HallP1
Symmetry operation#1: x,y,z

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Components

#1: Protein DNA damage-binding protein 1 / DDB p127 subunit / DNA damage-binding protein a / DDBa / Damage-specific DNA-binding protein 1 / ...DDB p127 subunit / DNA damage-binding protein a / DDBa / Damage-specific DNA-binding protein 1 / HBV X-associated protein 1 / XAP-1 / UV-damaged DNA-binding factor / UV-damaged DNA-binding protein 1 / UV-DDB 1 / XPE-binding factor / XPE-BF / Xeroderma pigmentosum group E-complementing protein / XPCe


Mass: 96033.945 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: DDB1, XAP1 / Cell line (production host): High Five / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q16531
#2: Protein Protein cereblon


Mass: 48976.117 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CRBN, AD-006 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q96SW2
#3: Protein Casein kinase I isoform alpha / CKI-alpha / CK1


Mass: 39302.238 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CSNK1A1 / Production host: Trichoplusia ni (cabbage looper)
References: UniProt: P48729, non-specific serine/threonine protein kinase
#4: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#5: Chemical ChemComp-YOT / (3S)-3-{5-[(1,2-benzoxazol-3-yl)amino]-1-oxo-1,3-dihydro-2H-isoindol-2-yl}piperidine-2,6-dione


Mass: 376.365 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C20H16N4O4 / Feature type: SUBJECT OF INVESTIGATION
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.74 Å3/Da / Density % sol: 55.14 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop / Details: 70 MM TRIS PH 7.0, 140 MM MGCL2, 7% W/V PEG 8000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Sep 29, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.45→50 Å / Num. obs: 49793 / % possible obs: 97.1 % / Observed criterion σ(I): -3 / Redundancy: 4.5 % / Biso Wilson estimate: 107.24 Å2 / CC1/2: 0.97 / Rmerge(I) obs: 0.177 / Rrim(I) all: 0.204 / Net I/σ(I): 8.56
Reflection shellResolution: 3.45→3.54 Å / Redundancy: 3.4 % / Mean I/σ(I) obs: 1.75 / Num. unique obs: 3410 / CC1/2: 0.708 / Rrim(I) all: 0.902 / Rsym value: 1.514 / % possible all: 90.2

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
XDSdata reduction
XDS0.7.3data scaling
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.45→48.25 Å / SU ML: 0.5124 / Cross valid method: FREE R-VALUE / σ(F): 1.93 / Phase error: 30.526
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2721 1998 4.02 %
Rwork0.213 47746 -
obs0.2153 49744 97.09 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 122.92 Å2
Refinement stepCycle: LAST / Resolution: 3.45→48.25 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms21267 0 30 0 21297
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.008821739
X-RAY DIFFRACTIONf_angle_d1.007729581
X-RAY DIFFRACTIONf_chiral_restr0.05373412
X-RAY DIFFRACTIONf_plane_restr0.00753803
X-RAY DIFFRACTIONf_dihedral_angle_d16.00917577
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.45-3.540.38451230.30583157X-RAY DIFFRACTION90.01
3.54-3.630.34571460.28093445X-RAY DIFFRACTION96.69
3.63-3.740.34841580.29533441X-RAY DIFFRACTION98.96
3.74-3.860.3471240.26533447X-RAY DIFFRACTION98.81
3.86-40.32151520.23633475X-RAY DIFFRACTION98.53
4-4.160.25091590.21683430X-RAY DIFFRACTION98.57
4.16-4.350.26531370.21363481X-RAY DIFFRACTION98.1
4.35-4.570.25521510.20953441X-RAY DIFFRACTION97.95
4.58-4.860.2531310.17823425X-RAY DIFFRACTION97.4
4.86-5.240.2721460.19343305X-RAY DIFFRACTION94.6
5.24-5.760.28571450.21133494X-RAY DIFFRACTION98.67
5.76-6.590.31261480.21683456X-RAY DIFFRACTION99.09
6.59-8.30.24581420.1993438X-RAY DIFFRACTION97.97
8.3-48.250.21671360.1863311X-RAY DIFFRACTION93.97
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.621422830809-0.2368277402380.1527432338050.886857535585-0.4399366516950.879082611365-0.0305158160055-0.158920605431-0.174948428288-0.119241605967-0.229221991572-0.11155576490.476711295422-0.270385576188-1.344458160990.917136500462-0.1122904259840.1184243065240.9181386617160.1266446890790.95499862643561.924-17.831-37.967
2-0.381417579454-0.389640994925-0.8195543961080.0110665109289-0.1024250199580.9322505273610.0275414609305-0.06518219488050.00654980318604-0.195711165861-0.186727166290.020484591643-0.0636329805843-0.1308538703-2.92564919888E-70.9026286063580.017911806180.04329808464940.8725614302920.03256260862680.84840883974764.1826.012-54.042
30.3269721772340.237536882194-0.1236079667310.409682711152-0.4023648862470.9471514769210.341714353561-0.207211159576-0.03257331069790.285497146281-0.202760292102-0.00593343908019-0.446216434743-0.342700755553-5.219448227370.9576873152650.1153862177980.1102649347440.936625173801-0.06757708872170.67678020711454.00523.525-37.062
40.155060337258-0.426582063416-0.2249727273850.3286246691610.1666545435380.2978441715850.153677071125-0.189153350237-0.160582634768-0.300797208579-0.09266243072840.145639351810.161705116054-0.07557063917924.15824581259E-60.746151959824-0.287544226248-0.05956704098531.67403222223-0.0005611781637030.97077319654133.48-1.242-41.73
50.484132201351-0.145950789160.2133784675480.2122230045820.0620262179161-0.2201686257550.13134733075-0.168383022266-0.140894052423-0.0546880276760.147090730715-0.04611096200610.215051314347-0.222539002844-7.7262407628E-60.9556226572840.0265510184128-0.02950964392990.9244361660250.09108485423710.87611479915252.4769.318.667
60.03573230974550.2052832822230.107306003542-0.1390458048950.1192863666840.0820042808623-0.503088761908-0.3162766712530.136395293305-0.2883398649040.31102286559-0.317223727653-0.1507131098710.6581800213050.0004289865719371.56677314439-0.313690996703-0.05746353430321.313543628180.1048535444451.3817881883765.7412.74-27.236
70.551740826114-0.3187721068250.2669132032870.0772294617150.1434122792310.136837371314-0.1647640852460.607465662392-0.07520443948860.2293085934140.4269175863130.03298485090750.1030809505060.125160375087-1.24221673201E-51.09760544871-0.0844113985876-0.01156460607481.15525512804-0.08198798865480.90323401231264.42714.188-15.87
80.4162331620470.659604628893-0.1278426101730.103256758464-0.8184324524580.000290418691647-0.34399811033-0.073645209059-0.155316832854-0.2621043687680.3848898405260.0617263439026-0.1789260375740.209581106512-1.37101713421E-51.05806276536-0.012741827511-0.03203620990790.9632883272430.01427497375630.95842401824970.20725.9476.271
90.003238508002820.08174826069920.02121713024640.008865399599490.00472212072524-0.022751978799-0.366136214433-0.0126373188855-0.156484818233-0.075301484392-0.1905304073690.1587245130160.7021998758590.309726344581-1.46363347217E-51.097148330730.2852783457080.0239966340090.9949497180960.01626749113740.96118594901260.77531.94728.056
100.05981886353310.287758423905-0.1356138156430.02116035204690.1054415437410.0801013303745-0.0379922192077-0.07896267626460.0065830702289-0.180661202530.121928970780.4340751577560.3185835155590.2770482803050.001892924324030.8726032364730.001666824571390.06313772693540.700059333530.04691703743620.98470706763557.36842.29124.912
110.19390731445-0.284339834693-0.3525936822440.0731433010812-0.5353947405060.2096681095150.090590310218-0.25393217040.198039317738-0.04233652132360.002365515065340.3456262667310.2104975467280.1560121503525.81475222625E-70.7159423747190.223818966006-0.04751487148790.957834586635-0.01987535739630.91819693966475.39850.37827.355
120.0969285070009-0.0104121543458-0.0210025496651-0.0365976252246-0.0366605741955-0.0149375968390.345941815759-0.3171443419030.4834368937920.192308259759-0.1908130180030.1005814282560.0899538591041-0.003550244657740.5812404820010.7535938735140.3129157777240.05669362545340.913930946636-0.05346083293451.5956197194859.46964.2424.418
13-0.06562965634470.02540043463050.00199742049670.0181000284175-0.0801866676806-0.02536088326560.495178599201-0.6170718211560.02675682066070.276575881464-0.466833033593-0.0652692144509-0.642201815336-0.07337016753095.007165846791.011993691410.2810999880020.1415580173261.305103468060.05438155598960.97740380424864.39964.69438.823
140.533345076387-0.316886196110.2420862423410.575840589706-0.7861413536820.03646935612380.0952648377106-0.3349428738660.2197845856320.1442207274040.1624103222780.105116922048-0.04588113496120.1891965776861.76315326272E-60.962804369480.0533191136874-0.03456515110571.26907661133-0.09546204450160.95980942487679.99161.10633.042
151.81220546076-0.005475236542130.3719857349571.07801119732-0.519875529151-0.0557400677348-0.145246009105-0.3562749589920.119413560928-0.2854331821220.0226122842384-0.107479147388-0.0383662027430.149804161380.001869098373050.6676697612180.0089369196317-0.02275886592320.721714279617-0.0004553613541650.8445381231348.116-37.4821.622
16-0.2851162650480.0656271273158-0.821591827911-0.154284580315-0.3510724867350.7954792550720.0414908145888-0.2662030847540.153591471132-0.0550753413908-0.002709001505180.3281441452920.1477006858510.334342211626-0.005042240712340.3525022282640.0640819853894-0.09785645953870.556364164529-0.0517110309220.64020148510818.501-43.6337.232
170.7939218221370.205077386608-0.2146211500890.641352746801-0.227788949770.824079109874-0.2156349857780.2384452033210.0414880744624-0.05128174842470.2478374416830.1363803733920.257791809942-0.09228286135837.23344561172E-60.717564223851-0.0847278890036-0.1627425124140.8491985882850.02922883750780.8337048828764.468-50.2-10.9
180.5368214406870.0148668856744-1.010147045870.2219647152790.8190148795210.1562517410490.01907723654670.1610071818760.276790773804-0.3718254500260.244674214463-0.179515513330.0881952587597-0.04317626655680.3080213428771.09295268258-0.22087731389-0.1557779565470.7010281976720.1508930514030.95220498539919.805-24.424-15.873
190.0325143541755-0.03933564785630.08540529474570.09385761437090.06094276059270.1101685150840.54078141621-0.6844916138730.823818889741-0.4426958653770.0524467794101-0.237732918198-0.2324161917060.9876297174591.03595944741E-51.56708329972-0.2460274236940.3461360560931.72313542356-0.1643432605191.2390341026936.729-50.36-49.638
20-0.2937214340680.2552281129550.2823585498410.303419428390.5017876117010.09137926383530.420603595574-0.420531755148-0.14968459447-0.1205774227230.6703911319820.5571052055570.865157109120.225422895714-0.006430721296051.381688699740.0433097767008-0.02333685425921.535206135720.01269841958741.4930968076733.066-48.248-21.211
210.1806496689710.1182075434970.2281673611920.07682249478090.0191196905650.0560274422205-0.409851136938-0.04002894461850.1723308591890.1513475486340.497601172290.276279339370.1060472482750.893076874111-1.96179657673E-51.15405402928-0.00536807414247-0.01819476094160.98743226635-0.07750409845320.90126121884126.843-54.877-17.324
220.108081732733-0.889192052367-0.1226218860850.3345173221630.92842870038-0.111805779313-0.3763828812870.057258806040.167027892514-0.3660445375670.6723907333470.0931831260583-0.06168777616860.341758343865-98.32034249231.18761263373-0.3153786447050.02601019555741.13634953565-0.1767489562280.89783692791526.421-62.107-39.206
230.1565653484350.09525698249640.04879106849580.136690346291-0.174547248792-0.069287127078-0.1558391372510.443290744133-0.06357462391080.08363026487960.2804828587580.39423950224-0.786327431860.945550213214-1.51155839329E-51.39650613473-0.3949390813870.04955672180891.51703437352-0.2245019845190.99387087669326.833-73.825-49.704
240.11549362414-0.143548644576-0.05346205098350.242734469435-0.0503799494030.02821479647750.3742636893930.3347129704680.01757368894160.750046961264-0.258465979080.244272618543-0.765077945560.499840921475-3.77593013902E-61.45269168768-0.135964687096-0.008860733202491.2692448741-0.1871967600420.80611074529725.916-71.593-71.886
250.058650526135-0.163720116016-0.1639065735310.05609229508410.04586972736210.04757426167130.3210214646510.04762749563680.1976828787170.1901000795860.02545209240060.0553033198203-0.4714137810160.281719495401-1.7279001572E-61.35544822866-0.105146770160.1355189112970.8156753608020.06263600180090.96774983809513.698-71.454-75.225
260.248883797251-0.0365215006419-0.002800093472910.358377805745-0.6409981575890.3981230999410.172558646489-0.162435468101-0.09127796711660.326401994858-0.103070578935-0.205224125317-0.3499464289980.0729623555594-16.53182049820.8538458182130.07936160741110.008347014927760.705073287314-0.05742166157380.82841323474213.708-88.636-71.678
270.510755929989-0.1070991362720.1658148433760.37864638776-0.08761425938630.815144662649-0.2687755710840.497584011314-0.0972518978121-0.1103858649480.2691895093990.1239061971850.000359919161816-0.255117581164-16.47591141550.8094184040150.01864651846260.04639657258070.784187089572-0.02369731745530.824493349044.261-92.498-85.711
280.2013116015880.0902585691453-0.6252197787930.203816651623-0.1161136796080.529648467528-0.398402420931-0.147712506808-0.164404792002-0.00281057718780.2039455512060.233954995880.180845688502-0.3589092384525.55109542676E-60.8469821677310.1221546617240.06794488024490.778482272598-0.00727565952490.9053115994835.258-100.41-69.689
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 2:299 )A2 - 299
2X-RAY DIFFRACTION2( CHAIN A AND RESID 300:784 )A300 - 784
3X-RAY DIFFRACTION3( CHAIN A AND RESID 785:1009 )A785 - 1009
4X-RAY DIFFRACTION4( CHAIN A AND RESID 1010:1140 )A1010 - 1140
5X-RAY DIFFRACTION5( CHAIN B AND RESID 47:184 )B47 - 184
6X-RAY DIFFRACTION6( CHAIN B AND RESID 185:231 )B185 - 231
7X-RAY DIFFRACTION7( CHAIN B AND RESID 232:323 )B232 - 323
8X-RAY DIFFRACTION8( CHAIN B AND RESID 324:428 )B324 - 428
9X-RAY DIFFRACTION9( CHAIN C AND RESID 11:36 )C11 - 36
10X-RAY DIFFRACTION10( CHAIN C AND RESID 37:90 )C37 - 90
11X-RAY DIFFRACTION11( CHAIN C AND RESID 91:155 )C91 - 155
12X-RAY DIFFRACTION12( CHAIN C AND RESID 156:175 )C156 - 175
13X-RAY DIFFRACTION13( CHAIN C AND RESID 176:199 )C176 - 199
14X-RAY DIFFRACTION14( CHAIN C AND RESID 200:303 )C200 - 303
15X-RAY DIFFRACTION15( CHAIN D AND RESID 2:307 )D2 - 307
16X-RAY DIFFRACTION16( CHAIN D AND RESID 308:795 )D308 - 795
17X-RAY DIFFRACTION17( CHAIN D AND RESID 796:928 )D796 - 928
18X-RAY DIFFRACTION18( CHAIN D AND RESID 929:1140 )D929 - 1140
19X-RAY DIFFRACTION19( CHAIN E AND RESID 86:161 )E86 - 161
20X-RAY DIFFRACTION20( CHAIN E AND RESID 162:231 )E162 - 231
21X-RAY DIFFRACTION21( CHAIN E AND RESID 232:264 )E232 - 264
22X-RAY DIFFRACTION22( CHAIN E AND RESID 265:371 )E265 - 371
23X-RAY DIFFRACTION23( CHAIN E AND RESID 372:427 )E372 - 427
24X-RAY DIFFRACTION24( CHAIN F AND RESID 11:41 )F11 - 41
25X-RAY DIFFRACTION25( CHAIN F AND RESID 42:84 )F42 - 84
26X-RAY DIFFRACTION26( CHAIN F AND RESID 85:155 )F85 - 155
27X-RAY DIFFRACTION27( CHAIN F AND RESID 156:253 )F156 - 253
28X-RAY DIFFRACTION28( CHAIN F AND RESID 254:303 )F254 - 303

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