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- PDB-8ft7: Crystal structure of SAH bound protein arginine N-methyltransfera... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8ft7 | |||||||||
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Title | Crystal structure of SAH bound protein arginine N-methyltransferase 1 (PRMT1) from Naegleria fowleri | |||||||||
![]() | PUA domain-containing protein | |||||||||
![]() | TRANSFERASE / SSGCID / STRUCTURAL GENOMICS / SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE | |||||||||
Function / homology | ![]() gamma-glutamylcyclotransferase activity / glutathione catabolic process / peptidyl-arginine methylation / protein-arginine N-methyltransferase activity / RNA binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Seattle Structural Genomics Center for Infectious Disease (SSGCID) | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of SAH bound protein arginine N-methyltransferase 1 (PRMT1) from Naegleria fowleri Authors: Seibold, S. / Battaile, K.P. / Lovell, S. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 143.2 KB | Display | ![]() |
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PDB format | ![]() | 107.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 889.9 KB | Display | ![]() |
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Full document | ![]() | 891.9 KB | Display | |
Data in XML | ![]() | 23.9 KB | Display | |
Data in CIF | ![]() | 33 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 41484.957 Da / Num. of mol.: 2 / Fragment: NafoA.20639.a.A2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: FDP41_006519 / Plasmid: NafoA.20639.a.A2 / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.95 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: Wizard Cryo H6: 30% PEG 200, 100mM Sodium Acetate pH 4.5, 100mM Sodium Chloride. NafoA.20639.a.A2.PW39094 at 8.5 mg/mL Tray: Steve 11/09/22 well D10, 24 hour soak in 5mM S- ...Details: Wizard Cryo H6: 30% PEG 200, 100mM Sodium Acetate pH 4.5, 100mM Sodium Chloride. NafoA.20639.a.A2.PW39094 at 8.5 mg/mL Tray: Steve 11/09/22 well D10, 24 hour soak in 5mM S-adenosylhomocysteine (SAH), Puck: PSL0710, Cryo: direct |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER2 XE 9M / Detector: PIXEL / Date: Dec 10, 2022 |
Radiation | Monochromator: Double Crystal Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97856 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→47.95 Å / Num. obs: 49721 / % possible obs: 100 % / Redundancy: 13.7 % / CC1/2: 0.999 / Rmerge(I) obs: 0.058 / Rpim(I) all: 0.016 / Rrim(I) all: 0.06 / Χ2: 1.01 / Net I/σ(I): 21.9 / Num. measured all: 680729 |
Reflection shell | Resolution: 2.15→2.22 Å / % possible obs: 100 % / Redundancy: 14.5 % / Rmerge(I) obs: 1.485 / Num. measured all: 61863 / Num. unique obs: 4272 / CC1/2: 0.768 / Rpim(I) all: 0.403 / Rrim(I) all: 1.539 / Χ2: 1.05 / Net I/σ(I) obs: 2.1 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.15→35.6 Å
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Refine LS restraints |
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LS refinement shell |
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