Uncharacterized protein / Intraflagellar transport protein 122B, putative / Wd repeat-containing protein 19 / Intraflagellar transport protein 43 / Intraflagellar transport protein 140 / Uncharacterized protein Similarity search - Component
Biological species
Leishmania tarentolae (eukaryote)
Method
ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.4 Å
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM141109
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM143183
United States
Citation
Journal: Cell / Year: 2022 Title: Mechanism of IFT-A polymerization into trains for ciliary transport. Authors: Shimi Meleppattu / Haixia Zhou / Jin Dai / Miao Gui / Alan Brown / Abstract: Intraflagellar transport (IFT) is the highly conserved process by which proteins are transported along ciliary microtubules by a train-like polymeric assembly of IFT-A and IFT-B complexes. IFT-A is ...Intraflagellar transport (IFT) is the highly conserved process by which proteins are transported along ciliary microtubules by a train-like polymeric assembly of IFT-A and IFT-B complexes. IFT-A is sandwiched between IFT-B and the ciliary membrane, consistent with its putative role in transporting transmembrane and membrane-associated cargoes. Here, we have used single-particle analysis electron cryomicroscopy (cryo-EM) to determine structures of native IFT-A complexes. We show that subcomplex rearrangements enable IFT-A to polymerize laterally on anterograde IFT trains, revealing a cooperative assembly mechanism. Surprisingly, we discover that binding of IFT-A to IFT-B shields the preferred lipid-binding interface from the ciliary membrane but orients an interconnected network of β-propeller domains with the capacity to accommodate diverse cargoes toward the ciliary membrane. This work provides a mechanistic basis for understanding IFT-train assembly and cargo interactions.
A: NET domain-containing protein B: Intraflagellar transport protein 122B, putative C: Intraflagellar transport protein 122 homolog D: TPR_REGION domain-containing protein E: WD_REPEATS_REGION domain-containing protein F: WD_REPEATS_REGION domain-containing protein hetero molecules
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