[English] 日本語
![](img/lk-miru.gif)
- PDB-8et4: Crystal structure of wild-type arabidopsis thaliana acetohydroxya... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 8et4 | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron | ||||||
![]() | Acetolactate synthase, chloroplastic | ||||||
![]() | TRANSFERASE / Herbicide / Resistance / AHAS / ALS / LIGASE | ||||||
Function / homology | ![]() acetolactate synthase / acetolactate synthase complex / acetolactate synthase activity / valine biosynthetic process / isoleucine biosynthetic process / response to herbicide / thiamine pyrophosphate binding / chloroplast stroma / amino acid biosynthetic process / chloroplast ...acetolactate synthase / acetolactate synthase complex / acetolactate synthase activity / valine biosynthetic process / isoleucine biosynthetic process / response to herbicide / thiamine pyrophosphate binding / chloroplast stroma / amino acid biosynthetic process / chloroplast / flavin adenine dinucleotide binding / magnesium ion binding / mitochondrion Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Guddat, L.W. / Cheng, Y. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Crystal Structure of the Commercial Herbicide, Amidosulfuron, in Complex with Arabidopsis thaliana Acetohydroxyacid Synthase. Authors: Cheng, Y. / Lonhienne, T. / Garcia, M.D. / Williams, C.M. / Schenk, G. / Guddat, L.W. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 163.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 100.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 24 KB | Display | |
Data in CIF | ![]() | 31.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8et5C ![]() 5k2oS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||||||
Unit cell |
| ||||||||||||
Components on special symmetry positions |
|
-
Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 64591.664 Da / Num. of mol.: 1 / Fragment: UNP residues 86-667 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|
-Non-polymers , 8 types, 11 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/FAD.gif)
![](data/chem/img/WRQ.gif)
![](data/chem/img/TLA.gif)
![](data/chem/img/NHE.gif)
![](data/chem/img/AUJ.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/FAD.gif)
![](data/chem/img/WRQ.gif)
![](data/chem/img/TLA.gif)
![](data/chem/img/NHE.gif)
![](data/chem/img/AUJ.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | ChemComp-FAD / | #4: Chemical | ChemComp-WRQ / | #5: Chemical | ChemComp-TLA / | #6: Chemical | ChemComp-NHE / | #7: Chemical | ChemComp-AUJ / | #8: Chemical | #9: Water | ChemComp-HOH / | |
---|
-Details
Has ligand of interest | N |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 6.74 Å3/Da / Density % sol: 81.74 % / Description: Yellow diamond shape |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 9.8 Details: CHES buffer, potassium sodium tartrate, sodium sulfate PH range: 9.4-9.8 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Jun 7, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.95→48.26 Å / Num. obs: 37363 / % possible obs: 99.7 % / Redundancy: 13.3 % / Biso Wilson estimate: 66.48 Å2 / CC1/2: 0.999 / Rpim(I) all: 0.032 / Net I/σ(I): 17.3 |
Reflection shell | Resolution: 2.95→3.08 Å / Redundancy: 13.3 % / Num. unique obs: 4409 / CC1/2: 0.918 / Rpim(I) all: 0.251 / % possible all: 98.2 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 5K2O Resolution: 2.95→48.26 Å / SU ML: 0.3071 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 20.6527 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 60.6 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.95→48.26 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|