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- PDB-8emb: X-ray crystal structure of Thermosynechococcus elongatus Si3 doma... -

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Basic information

Entry
Database: PDB / ID: 8emb
TitleX-ray crystal structure of Thermosynechococcus elongatus Si3 domain of RNA polymerase RpoC2 subunit
ComponentsDNA-directed RNA polymerase subunit beta'Polymerase
KeywordsTRANSCRIPTION / RNA polymerase / Lineage specific insertion / trigger loop
Function / homology
Function and homology information


DNA-directed RNA polymerase complex / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / DNA-templated transcription / DNA binding / zinc ion binding
Similarity search - Function
DNA-directed RNA polymerase, subunit beta'' / RNA polymerase Rpb1, domain 3 superfamily / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 5
Similarity search - Domain/homology
DNA-directed RNA polymerase subunit beta'
Similarity search - Component
Biological speciesThermosynechococcus vestitus BP-1 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.06 Å
AuthorsMurakami, K.S. / Imashimizu, M. / Qayyum, M.Z. / Vishwakarma, R.K. / Yuzenkova, Y.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM131860 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM087350 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2024
Title: Structure and function of the Si3 insertion integrated into the trigger loop/helix of cyanobacterial RNA polymerase.
Authors: M Zuhaib Qayyum / Masahiko Imashimizu / Miron Leanca / Rishi K Vishwakarma / Amber Riaz-Bradley / Yulia Yuzenkova / Katsuhiko S Murakami /
Abstract: Cyanobacteria and evolutionarily related chloroplasts of algae and plants possess unique RNA polymerases (RNAPs) with characteristics that distinguish them from canonical bacterial RNAPs. The largest ...Cyanobacteria and evolutionarily related chloroplasts of algae and plants possess unique RNA polymerases (RNAPs) with characteristics that distinguish them from canonical bacterial RNAPs. The largest subunit of cyanobacterial RNAP (cyRNAP) is divided into two polypeptides, β'1 and β'2, and contains the largest known lineage-specific insertion domain, Si3, located in the middle of the trigger loop and spanning approximately half of the β'2 subunit. In this study, we present the X-ray crystal structure of Si3 and the cryo-EM structures of the cyRNAP transcription elongation complex plus the NusG factor with and without incoming nucleoside triphosphate (iNTP) bound at the active site. Si3 has a well-ordered and elongated shape that exceeds the length of the main body of cyRNAP, fits into cavities of cyRNAP in the absence of iNTP bound at the active site and shields the binding site of secondary channel-binding proteins such as Gre and DksA. A small transition from the trigger loop to the trigger helix upon iNTP binding results in a large swing motion of Si3; however, this transition does not affect the catalytic activity of cyRNAP due to its minimal contact with cyRNAP, NusG, or DNA. This study provides a structural framework for understanding the evolutionary significance of these features unique to cyRNAP and chloroplast RNAP and may provide insights into the molecular mechanism of transcription in specific environment of photosynthetic organisms and organelle.
History
DepositionSep 27, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 9, 2022Provider: repository / Type: Initial release
Revision 1.1Feb 21, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA-directed RNA polymerase subunit beta'
B: DNA-directed RNA polymerase subunit beta'
C: DNA-directed RNA polymerase subunit beta'
D: DNA-directed RNA polymerase subunit beta'
E: DNA-directed RNA polymerase subunit beta'
F: DNA-directed RNA polymerase subunit beta'


Theoretical massNumber of molelcules
Total (without water)361,1476
Polymers361,1476
Non-polymers00
Water0
1
A: DNA-directed RNA polymerase subunit beta'


Theoretical massNumber of molelcules
Total (without water)60,1911
Polymers60,1911
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: DNA-directed RNA polymerase subunit beta'


Theoretical massNumber of molelcules
Total (without water)60,1911
Polymers60,1911
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: DNA-directed RNA polymerase subunit beta'


Theoretical massNumber of molelcules
Total (without water)60,1911
Polymers60,1911
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: DNA-directed RNA polymerase subunit beta'


Theoretical massNumber of molelcules
Total (without water)60,1911
Polymers60,1911
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: DNA-directed RNA polymerase subunit beta'


Theoretical massNumber of molelcules
Total (without water)60,1911
Polymers60,1911
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: DNA-directed RNA polymerase subunit beta'


Theoretical massNumber of molelcules
Total (without water)60,1911
Polymers60,1911
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)178.663, 178.663, 281.038
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number154
Space group name H-MP3221
Space group name HallP322"
Symmetry operation#1: x,y,z
#2: -y,x-y,z+2/3
#3: -x+y,-x,z+1/3
#4: x-y,-y,-z+1/3
#5: -x,-x+y,-z+2/3
#6: y,x,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 440 through 798 or resid 808 through 977))
d_2ens_1(chain "B" and resid 440 through 977)
d_3ens_1(chain "C" and (resid 440 through 798 or resid 808 through 977))
d_4ens_1(chain "D" and (resid 440 through 798 or resid 808 through 977))

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1THRILEA7 - 365
d_12ens_1ASPPHEA375 - 544
d_21ens_1THRPHEB7 - 535
d_31ens_1THRILEC1 - 359
d_32ens_1ASPPHEC362 - 531
d_41ens_1THRILED4 - 362
d_42ens_1ASPPHED372 - 541

NCS oper:
IDCodeMatrixVector
1given(-0.9912474505, -0.129704468105, 0.0246016834901), (-0.12943159318, 0.991511015716, 0.0123841996604), (-0.0259991262154, 0.00909157125079, -0.99962062242)110.257332111, -52.2510106831, 471.510429317
2given(0.989464396154, 0.134126700644, -0.0544998799718), (-0.137179881628, 0.988917601919, -0.0567772550553), (0.0462805447144, 0.0636553594719, 0.996898242747)-12.4208357901, -90.8381694468, -14.3958081299
3given(0.581819555948, 0.800248648931, -0.145217437663), (-0.80911254451, 0.587643775678, -0.00341807312008), (0.0826008149661, 0.119485952279, 0.989393861197)-135.786792366, 79.6698738905, -26.0799815274

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Components

#1: Protein
DNA-directed RNA polymerase subunit beta' / Polymerase / RNAP subunit beta' / RNA polymerase subunit beta' / Transcriptase subunit beta'


Mass: 60191.102 Da / Num. of mol.: 6 / Mutation: L508M, L738M, A922M
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermosynechococcus vestitus BP-1 (bacteria)
Strain: NIES-2133 / IAM M-273 / BP-1 / Gene: rpoC2, tll0640 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8DL57, DNA-directed RNA polymerase
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.59 Å3/Da / Density % sol: 71.34 %
Crystal growTemperature: 278 K / Method: vapor diffusion, hanging drop / pH: 5
Details: 0.1 M sodium citrate, pH 5, 0.4-0.6 M magnesium chloride, 4-6% PEG3350, 50 ug/mL heparin

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 0.9788 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 26, 2006
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9788 Å / Relative weight: 1
ReflectionResolution: 3.06→50 Å / Num. obs: 96754 / % possible obs: 98.14 % / Redundancy: 12 % / Biso Wilson estimate: 88.96 Å2 / CC1/2: 0.996 / Rpim(I) all: 0.06 / Rrim(I) all: 0.209 / Χ2: 1.724 / Net I/σ(I): 17
Reflection shellResolution: 3.07→3.12 Å / Redundancy: 9.5 % / Num. unique obs: 4161 / CC1/2: 0.263 / Χ2: 1.319 / % possible all: 85.8

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
PHENIX1.20.1_4487refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: SAD / Resolution: 3.06→49.61 Å / SU ML: 0.4902 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 33.3571
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2845 3813 2.06 %
Rwork0.2644 181249 -
obs0.2648 96540 98.14 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 130.14 Å2
Refinement stepCycle: LAST / Resolution: 3.06→49.61 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms21086 0 0 0 21086
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.003321349
X-RAY DIFFRACTIONf_angle_d0.749628943
X-RAY DIFFRACTIONf_chiral_restr0.05313444
X-RAY DIFFRACTIONf_plane_restr0.00433822
X-RAY DIFFRACTIONf_dihedral_angle_d16.86258145
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS1.93391055876
ens_1d_3AX-RAY DIFFRACTIONTorsion NCS3.60984270944
ens_1d_4AX-RAY DIFFRACTIONTorsion NCS3.25568545146
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.06-3.10.37091120.38995270X-RAY DIFFRACTION76.37
3.1-3.140.42591210.38375814X-RAY DIFFRACTION84.8
3.14-3.190.3941340.37756392X-RAY DIFFRACTION93.59
3.19-3.230.42531440.39356767X-RAY DIFFRACTION99.24
3.23-3.280.4191450.37876725X-RAY DIFFRACTION99.42
3.28-3.330.39881420.35316937X-RAY DIFFRACTION99.58
3.33-3.390.35421400.34776725X-RAY DIFFRACTION99.75
3.39-3.440.34461440.3296912X-RAY DIFFRACTION99.79
3.44-3.510.31591480.31196835X-RAY DIFFRACTION99.86
3.51-3.570.33841470.30876827X-RAY DIFFRACTION99.67
3.58-3.650.36811460.2916764X-RAY DIFFRACTION99.77
3.65-3.730.32031410.29086844X-RAY DIFFRACTION99.8
3.73-3.810.31061410.30116807X-RAY DIFFRACTION99.83
3.81-3.910.3491440.28326835X-RAY DIFFRACTION99.84
3.91-4.010.29261360.25326838X-RAY DIFFRACTION99.93
4.02-4.130.27741440.24596817X-RAY DIFFRACTION99.93
4.13-4.270.26981420.23566868X-RAY DIFFRACTION99.94
4.27-4.420.23661440.22316786X-RAY DIFFRACTION99.9
4.42-4.60.23651410.21816877X-RAY DIFFRACTION99.96
4.6-4.80.20131360.2166785X-RAY DIFFRACTION99.93
4.8-5.060.29131440.23226837X-RAY DIFFRACTION99.91
5.06-5.370.26091440.23186833X-RAY DIFFRACTION99.94
5.37-5.790.24891520.26636814X-RAY DIFFRACTION99.93
5.79-6.370.29531440.28526865X-RAY DIFFRACTION99.96
6.37-7.290.32921400.27756822X-RAY DIFFRACTION99.99
7.29-9.170.26281500.24426823X-RAY DIFFRACTION100
9.17-49.610.21081470.22366830X-RAY DIFFRACTION99.53
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.95054973023-1.955859768240.1107582054647.059133188352.379479105487.251148138730.009880433891990.221575319437-0.371892523437-0.4814614442430.06538067167290.4021671309250.151007013388-0.190902247866-0.09042432456510.394683786275-0.13592405717-0.1355131173470.5795955167020.03170890851510.84809315458450.4607502009196.899639514236.616962673
24.48107359136-3.793031157882.555221926476.4817773168-4.746311007537.33324058496-0.501068995989-0.1373287675650.4846087215580.3106641361660.3374752320190.288232253451-1.07855517459-1.058374107810.09798832565141.167218041740.409737502833-0.1236438339360.984989942295-0.02867203386541.1417231411231.3849314897225.418110818239.031230109
32.17259904688-0.175438085451.246367985563.34055991849-1.485831527043.50896697728-0.357934553976-0.405436373230.2467718655881.030933743210.103494529027-0.201399326231-0.61215328123-0.165139181770.2194327643421.407884621710.336533622512-0.1200874822930.995881978159-0.04007535209510.7768422747979.80022877342243.699446671201.224247995
48.29447026562.190304149312.537794883737.63757972554-1.748882634178.00035922158-0.06614946004030.493595633589-0.1410878929920.1834483508290.161643381145-0.3423388953430.7161168911860.0898125060437-0.08056521775940.7842664738860.183683045391-0.01269579834370.8281815164240.03959736419950.637576649677-6.71280412538241.045510762168.308215863
55.927823523411.967827496151.274271490943.409725881890.9167121589076.27344030596-0.237916839812-0.7427204347590.7035836550590.8287953254480.093905127991-0.528296574745-0.979822179930.7854309148470.1469370108310.959072220886-0.0516788744357-0.1282006534780.94057291015-0.09408447021150.78121933152850.6306647158204.51015458264.579754734
65.229666757322.579450890190.2203929863217.02372602088-2.600276076246.815519794490.0433527205507-0.716256261438-0.1358334773160.8979643599370.201033522622-0.07923199006630.3361981963810.0275572017218-0.1914551376390.6408169462430.101619125211-0.095074779080.377833266652-0.01203379684670.44241535905339.8429463985139.872637137235.700188084
71.356893743842.479248437692.539113627096.809010550555.268113556898.6050270282-0.1516841509870.1090476205120.130011141810.3214428982890.292406370526-0.5819741195510.1993830473920.704403961629-0.1268258521780.518949052964-0.0218684931021-0.1210654220960.4885186232330.005513860974290.88653477774756.058506568170.191181068233.769748311
80.836084126226-0.3698016767713.667927355135.47144447251-0.0570815706668.258167929170.19065691447-0.0246500762016-0.002410929792030.0654703856631-0.200288846035-0.1492236928610.496330716804-0.124882007127-0.01154268597430.97674360658-0.105195707124-0.3269291724071.35547830621-0.1572928944290.98895062926873.9727916322191.247417286272.726141133
95.67981613933-2.24618166105-0.3522337407168.993652649723.733854078179.443261011440.380552510086-0.00405492478504-1.33079827907-0.573357371288-0.01613817271731.009862138381.620778633960.0178590738237-0.3671212343991.23773804076-0.15608190007-0.4419457245870.847296617949-0.01788269189820.93157175004990.2448120419189.306608738305.372075338
102.69645245667-1.534445616061.133567726543.954766554533.670619390019.03968912222-0.7831007178651.425380919930.361857344819-1.554864534060.6224311563880.16941704769-0.9676429174910.2787432645420.1905898432321.23801544003-0.290466901857-0.008782897733110.674237347470.05372193091030.76758965796141.2794759841146.465997217207.892183555
119.37096221507-3.160227267131.481077999058.572628612075.302687847373.05647322831-0.2027217916470.802657206393-0.325084764009-0.6766632069080.405748570205-1.058473032020.2846936465351.2412933397-0.2268253494980.745751288162-0.006921608539650.1039270169340.722790301620.2334546871820.89044377880153.786573433685.562816358241.643294309
121.26152263316-3.075031691942.905946751237.70277397866-7.564683929959.29888037010.0758509993558-0.211996922212-0.34435770046-0.0950888608028-0.008241619024950.03124359326320.364790537291-0.186483107074-0.06925476542980.63615266397-0.0251787065775-0.1008081228860.4549272767230.1112899273990.62816669265636.0227555997112.520806783238.460771882
133.43913660089-1.79090557885.141596354932.50347568194-2.99045272666.62180408350.3666235464460.312729786854-0.31660070251-0.304690558237-0.2399621068190.1096253929290.8576272562090.439297817212-0.1544727644580.6449180509240.00624259733228-0.0009274082819880.399132497798-0.01930962322590.46093142479319.2532325088137.214915663200.421071712
146.343916958342.661073400832.477915242629.06085344486-0.05305866290567.433956061110.656184517450.846797614645-0.521483378255-1.14104207632-0.2158470601730.0596971108631.402759062250.311380840626-0.3094113781740.7580836835890.0929564017392-0.2039118330130.497365595688-0.1356375138130.4924876994421.85101314408141.313389492168.58630996
154.460727916272.62834855029-0.7606768966773.238352498272.289148600915.07192900047-0.373575310975-0.1523775067580.369260956970.3229958093140.2130955354380.0651506413254-0.8554784813740.8411494970440.2775942065660.9118220866030.0697533218117-0.1288531080410.5987669960130.180233605350.61817525595748.910893273890.970734012267.209481077
163.14219215387-1.89071709627-3.158074821617.134401111316.108159522863.58420908289-0.08891110322140.0247555828855-0.07146449546350.624816089608-0.2810530947331.124960352630.448455742028-0.4201244064210.3593928563070.632011728683-0.03951412436470.1502639476320.6445860606630.1008586152380.69685701056817.9289402151151.89334253239.814048855
170.8161257994111.38404338781-2.233073019464.80774338722-3.740004442448.837888936610.02792563074350.6029134098990.4438960781050.198466509919-0.342878555963-0.0340192981219-0.6643794059480.4518408253570.3422908658820.623648794266-0.139551096473-0.1268054743921.014048618640.2744517423761.1723619016527.5001311369184.818075572239.483962762
182.531232590970.323673858676-0.363513617951-0.0642023519962-0.5404170476156.253952479110.3758819851060.032606125551-0.146234292501-0.490608391171-0.489533370924-0.2483263925270.3194418475040.4467714064630.1251961331481.652760733250.0586858472050.1127858957311.273611803270.5736529013491.447606484234.4879051043213.950517084201.939612087
194.80218551705-0.8102316534310.7328602545015.44387393090.2571925066096.67789173251-0.152938618971-0.591785825277-1.10063462999-0.164121824376-0.2542787298540.01447712639691.498879593140.4855957355030.4005788078141.537193318260.002451617833880.07098420364131.154343817060.5279053955221.3195591596430.4430541612228.954683279167.849574508
207.06797229711-1.46220379113.159745430145.034950987033.914731298556.242536555310.369765966875-0.5658495683420.1192445232491.13912737453-0.009392354340680.1203167439480.561098754041-0.126714173734-0.3366794024710.976853323136-0.05651480612610.2706466491970.8935146003160.1361018078330.82864709746620.66751691159.236581883266.639595327
213.34158238537-0.9459963373061.768611660391.261356750040.2799917473466.713279385370.714877363696-2.28924462956-1.281669702870.786437043330.2924654770950.4840312908512.170626471-0.522754447692-1.048307374942.10157611527-0.319270538427-0.1861251988752.158386093740.3428385284131.3975271017810.4702224372119.854756342240.689254585
221.65339852069-0.767063082141-0.785800725293.880165293460.01865733630125.793198414380.08851738117380.112584990288-0.373227515839-0.301942029605-0.4423611251140.3781498601810.841027481479-0.2161036221590.4388077246861.49176527155-0.307089036024-0.02015861318711.2065797854-0.0325057634581.216544133625.83998779229112.236515898202.558536972
234.44777910460.550709818374-4.560442825732.223330947121.309084214779.40844775794-0.2736186361331.06173979074-0.766530385270.5057601994620.221781293817-0.695639832344-0.421823977574-0.8098403902590.1686724817132.539749114170.304954846038-0.3003819912531.82548857054-0.298086608681.60725822957.7965665687496.5927777558168.78297589
242.49822202036-2.023932801491.375984933791.44095513342-0.9006110109360.699309636816-0.140477658651-0.2781362499180.0648587249477-0.631110167496-0.0549794759502-0.4725644963610.770065825079-0.02261833805280.1915387301232.20751490636-0.3222866051490.5406224216441.442093342440.0185798321681.914441850963.2545562115150.384854967298.909888354
250.162784845748-0.2457192012171.009597988360.627482866174-2.026411722823.63898981210.356933847714-0.1085181172210.0779958638622-0.4211708062570.2003071866150.1238385510390.461929660435-0.0214401786642-0.4485788949812.23566872686-0.0543302562936-0.005020555476171.56456065025-0.007344373795291.6337989262315.7622348474123.567704875311.172505722
26-0.04156747473470.111283254605-0.01377191573790.02524442601550.1199275220380.233867942807-0.146760407801-0.2032065117820.2766588925570.155302888470.7532395763270.2621554298710.1306598850361.23422158925-0.7352728278682.44593628418-0.1542975166870.3722099403352.16835523350.2697807917941.4932524968516.2531528962140.869201446278.003170326
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 434:481 or resid 947:983)AA434 - 9831 - 550
22chain 'A' and (resid 482:538 or resid 828:894)AA482 - 89449 - 461
33chain 'A' and (resid 539:630 or resid 741:827)AA539 - 827106 - 394
44chain 'A' and (resid 631:740)AA631 - 740198 - 307
55chain 'A' and (resid 895:946)AA895 - 946462 - 513
66chain 'B' and (resid 434:481 or resid 947:983)BB434 - 9831 - 541
77chain 'B' and (resid 482:538 or resid 828:894)BB482 - 89449 - 452
88chain 'B' and (resid 539:630 or resid 741:827)BB539 - 827106 - 385
99chain 'B' and (resid 631:740)BB631 - 740198 - 307
1010chain 'B' and (resid 895:946)BB895 - 946453 - 504
1111chain 'C' and (resid 440:481 or resid 947:977)CC440 - 9771 - 531
1212chain 'C' and (resid 482:538 or resid 828:894)CC482 - 89443 - 448
1313chain 'C' and (resid 539:630 or resid 741:827)CC539 - 827100 - 381
1414chain 'C' and (resid 631:740)CC631 - 740192 - 301
1515chain 'C' and (resid 895:946)CC895 - 946449 - 500
1616chain 'D' and (resid 437:481 or resid 947:979)DD437 - 9791 - 543
1717chain 'D' and (resid 482:538 or resid 828:894)DD482 - 89446 - 458
1818chain 'D' and (resid 539:630 or resid 741:827)DD539 - 827103 - 391
1919chain 'D' and (resid 631:740)DD631 - 740195 - 304
2020chain 'D' and (resid 895:946)DD895 - 946459 - 510
2121chain 'E' and (resid 511:538 or resid 828:868)EE511 - 8681 - 358
2222chain 'E' and (resid 539:630 or resid 741:827)EE539 - 82729 - 317
2323chain 'E' and (resid 631:740)EE631 - 740121 - 230
2424chain 'F' and (resid 440:481 or resid 947:983)FF440 - 9761 - 246
2525chain 'F' and (resid 482:537 or resid 829:894)FF482 - 89443 - 164
2626chain 'F' and (resid 895:946)FF895 - 946165 - 216

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