+Open data
-Basic information
Entry | Database: PDB / ID: 8egw | ||||||
---|---|---|---|---|---|---|---|
Title | Complex of Fat4(EC1-4) bound to Dchs1(EC1-3) | ||||||
Components |
| ||||||
Keywords | CELL ADHESION / Fat4 / Dachsous1 / Cadherin / Protocadherin / Adhesion / Fat / Dachsous | ||||||
Function / homology | Function and homology information condensed mesenchymal cell proliferation / mitral valve formation / cell migration involved in endocardial cushion formation / cell-cell adhesion via plasma-membrane adhesion molecules / hippo signaling / pattern specification process / calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules / post-anal tail morphogenesis / digestive tract development / catenin complex ...condensed mesenchymal cell proliferation / mitral valve formation / cell migration involved in endocardial cushion formation / cell-cell adhesion via plasma-membrane adhesion molecules / hippo signaling / pattern specification process / calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules / post-anal tail morphogenesis / digestive tract development / catenin complex / cell-cell junction assembly / adherens junction organization / ossification involved in bone maturation / neural tube development / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules / branching involved in ureteric bud morphogenesis / cochlea development / homophilic cell adhesion via plasma membrane adhesion molecules / neurogenesis / protein localization to plasma membrane / adherens junction / cell morphogenesis / cell-cell adhesion / cerebral cortex development / apical part of cell / cadherin binding / calcium ion binding / extracellular exosome / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Medina, E. / Luca, V.C. | ||||||
Funding support | United States, 1items
| ||||||
Citation | Journal: Nat Commun / Year: 2023 Title: Structure of the planar cell polarity cadherins Fat4 and Dachsous1. Authors: Medina, E. / Easa, Y. / Lester, D.K. / Lau, E.K. / Sprinzak, D. / Luca, V.C. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 8egw.cif.gz | 317.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb8egw.ent.gz | 252.7 KB | Display | PDB format |
PDBx/mmJSON format | 8egw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/eg/8egw ftp://data.pdbj.org/pub/pdb/validation_reports/eg/8egw | HTTPS FTP |
---|
-Related structure data
Related structure data | 8egxC 2a4cS 3q2wS 4zi8S 4zplS 4zpoS 5iu9S S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 33863.098 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: DCHS1, CDH19, CDH25, FIB1, KIAA1773, PCDH16 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q96JQ0 |
---|---|
#2: Protein | Mass: 50777.395 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FAT4, CDHF14, FATJ, Nbla00548 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q6V0I7 |
-Sugars , 4 types, 5 molecules
#3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Polysaccharide | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #5: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #6: Sugar | ChemComp-NAG / | |
---|
-Non-polymers , 4 types, 314 molecules
#7: Chemical | ChemComp-CA / #8: Chemical | #9: Chemical | ChemComp-EDO / #10: Water | ChemComp-HOH / | |
---|
-Details
Has ligand of interest | N |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.91 % |
---|---|
Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop Details: 0.08 M Magnesium Chloride; 0.1 M Sodium Cacodylate, pH 6.1; 20% Polyethylene Glycol 1000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-BM / Wavelength: 1 Å |
Detector | Type: RAYONIX MX-300 / Detector: CCD / Date: Apr 11, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.204→49.085 Å / Num. obs: 50558 / % possible obs: 99.27 % / Redundancy: 3.4 % / CC1/2: 0.995 / Net I/σ(I): 8.87 |
Reflection shell | Resolution: 2.204→2.283 Å / Num. unique obs: 5002 / CC1/2: 0.8 / % possible all: 98.24 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2A4C, 4ZPL, 4ZI8, 4ZPO, 5IU9, 3Q2W Resolution: 2.3→49.083 Å / SU ML: 0.34 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 27.43 / Stereochemistry target values: ML
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→49.083 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group |
|