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Open data
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Basic information
Entry | Database: PDB / ID: 8eek | ||||||
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Title | C. ammoniagenes monoamine oxidase (MAO) bound to tyramine | ||||||
![]() | Amine oxidase | ||||||
![]() | FLAVOPROTEIN / Monoamine / oxidase / flavoenzyme / substrate | ||||||
Function / homology | Amine oxidase / Flavin containing amine oxidoreductase / FAD/NAD(P)-binding domain superfamily / oxidoreductase activity / 4-(2-aminoethyl)phenol / FLAVIN-ADENINE DINUCLEOTIDE / Amine oxidase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Muellers, S.N. / Allen, K.N. | ||||||
Funding support | 1items
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![]() | ![]() Title: Structural Insights into the Substrate Range of a Bacterial Monoamine Oxidase. Authors: Muellers, S.N. / Tararina, M.A. / Kuzmanovic, U. / Galagan, J.E. / Allen, K.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 193.6 KB | Display | ![]() |
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PDB format | ![]() | 151.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 933.3 KB | Display | ![]() |
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Full document | ![]() | 947.4 KB | Display | |
Data in XML | ![]() | 38.7 KB | Display | |
Data in CIF | ![]() | 56.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8eefC ![]() 8eegC ![]() 8eehC ![]() 8eeiC ![]() 8eejC ![]() 8eelC ![]() 8eemC ![]() 8eenC ![]() 8eeoC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50022.473 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: CA40472_00570 / Production host: ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 50.98 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop Details: 10 - 15% w/v PEG-3350, 0.1 M sodium malonate, pH 5.0 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 1, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.977 Å / Relative weight: 1 |
Reflection | Resolution: 1.51→38.07 Å / Num. obs: 157374 / % possible obs: 99.2 % / Redundancy: 25.8 % / Biso Wilson estimate: 16.51 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.272 / Rpim(I) all: 0.054 / Rrim(I) all: 0.278 / Net I/σ(I): 15 |
Reflection shell | Resolution: 1.51→1.564 Å / Redundancy: 25.2 % / Rmerge(I) obs: 1.371 / Mean I/σ(I) obs: 1.1 / Num. unique obs: 15566 / CC1/2: 0.643 / Rpim(I) all: 0.489 / % possible all: 99.34 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GEOSTD + MONOMER LIBRARY + CDL V1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.26 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.51→38.07 Å
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Refine LS restraints |
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LS refinement shell |
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