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- PDB-8dn7: The crystal structure of the Pisum sativum Toc75 POTRA domains in... -

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Basic information

Entry
Database: PDB / ID: 8dn7
TitleThe crystal structure of the Pisum sativum Toc75 POTRA domains in complex with fab ax9
Components
  • Protein TOC75, chloroplastic
  • fabax9 Heavy Chain
  • fabax9 Light Chain
KeywordsMembrane Protein/Immune System / Membrane Protein / Chloroplast / Membrane Protein-Immune System complex
Function / homology
Function and homology information


chloroplast outer membrane / protein-transporting ATPase activity / intracellular protein transport
Similarity search - Function
Chloroplast envelope protein translocase, IAP75 / Surface antigen D15-like / Bacterial surface antigen (D15) / Omp85 superfamily domain / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Protein TOC75, chloroplastic
Similarity search - Component
Biological speciesPisum sativum (garden pea)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2 Å
AuthorsSrinivasan, K. / Noinaj, N.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM127896-01 United States
CitationJournal: Structure / Year: 2023
Title: Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum.
Authors: Srinivasan, K. / Erramilli, S.K. / Chakravarthy, S. / Gonzalez, A. / Kossiakoff, A. / Noinaj, N.
History
DepositionJul 10, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 17, 2023Provider: repository / Type: Initial release
Revision 1.1Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.2Nov 20, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
E: Protein TOC75, chloroplastic
C: Protein TOC75, chloroplastic
D: fabax9 Heavy Chain
A: fabax9 Heavy Chain
B: fabax9 Light Chain
H: fabax9 Light Chain


Theoretical massNumber of molelcules
Total (without water)171,1806
Polymers171,1806
Non-polymers00
Water7,602422
1
E: Protein TOC75, chloroplastic
D: fabax9 Heavy Chain
H: fabax9 Light Chain


Theoretical massNumber of molelcules
Total (without water)85,5903
Polymers85,5903
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6380 Å2
ΔGint-44 kcal/mol
Surface area24820 Å2
MethodPISA
2
C: Protein TOC75, chloroplastic
A: fabax9 Heavy Chain
B: fabax9 Light Chain


Theoretical massNumber of molelcules
Total (without water)85,5903
Polymers85,5903
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5940 Å2
ΔGint-44 kcal/mol
Surface area25430 Å2
MethodPISA
Unit cell
Length a, b, c (Å)43.212, 43.430, 169.606
Angle α, β, γ (deg.)94.200, 94.210, 104.440
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 5 through 194 or resid 196 through 231))
21(chain D and (resid 5 through 132 or (resid 133...
12(chain B and (resid 3 through 10 or resid 12 or resid 14 through 158 or resid 160 through 216))
22(chain H and (resid 3 through 10 or resid 12...
13(chain C and (resid 114 or (resid 115 and (name...
23(chain E and resid 114 through 228)

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111VALVALLEULEU(chain A and (resid 5 through 194 or resid 196 through 231))AD5 - 1945 - 194
121SERSERSERSER(chain A and (resid 5 through 194 or resid 196 through 231))AD196 - 231196 - 231
211VALVALTHRTHR(chain D and (resid 5 through 132 or (resid 133...DC5 - 1325 - 132
221LYSLYSLYSLYS(chain D and (resid 5 through 132 or (resid 133...DC133133
231VALVALCYSCYS(chain D and (resid 5 through 132 or (resid 133...DC5 - 2325 - 232
241VALVALCYSCYS(chain D and (resid 5 through 132 or (resid 133...DC5 - 2325 - 232
251VALVALCYSCYS(chain D and (resid 5 through 132 or (resid 133...DC5 - 2325 - 232
261VALVALCYSCYS(chain D and (resid 5 through 132 or (resid 133...DC5 - 2325 - 232
112ILEILESERSER(chain B and (resid 3 through 10 or resid 12 or resid 14 through 158 or resid 160 through 216))BE3 - 103 - 10
122LEULEULEULEU(chain B and (resid 3 through 10 or resid 12 or resid 14 through 158 or resid 160 through 216))BE1212
132ALAALAGLNGLN(chain B and (resid 3 through 10 or resid 12 or resid 14 through 158 or resid 160 through 216))BE14 - 15814 - 158
142GLYGLYGLUGLU(chain B and (resid 3 through 10 or resid 12 or resid 14 through 158 or resid 160 through 216))BE160 - 216160 - 216
212ILEILESERSER(chain H and (resid 3 through 10 or resid 12...HF3 - 103 - 10
222LEULEULEULEU(chain H and (resid 3 through 10 or resid 12...HF1212
232ALAALAALAALA(chain H and (resid 3 through 10 or resid 12...HF14 - 2614 - 26
242SERSERGLNGLN(chain H and (resid 3 through 10 or resid 12...HF27 - 2827 - 28
252ILEILECYSCYS(chain H and (resid 3 through 10 or resid 12...HF3 - 2173 - 217
262ILEILECYSCYS(chain H and (resid 3 through 10 or resid 12...HF3 - 2173 - 217
272ILEILECYSCYS(chain H and (resid 3 through 10 or resid 12...HF3 - 2173 - 217
113ALAALAALAALA(chain C and (resid 114 or (resid 115 and (name...CB114115
123ASPASPASPASP(chain C and (resid 114 or (resid 115 and (name...CB115116
133ALAALALYSLYS(chain C and (resid 114 or (resid 115 and (name...CB114 - 231115 - 232
143ALAALALYSLYS(chain C and (resid 114 or (resid 115 and (name...CB114 - 231115 - 232
153ALAALALYSLYS(chain C and (resid 114 or (resid 115 and (name...CB114 - 231115 - 232
163ALAALALYSLYS(chain C and (resid 114 or (resid 115 and (name...CB114 - 231115 - 232
173ALAALALYSLYS(chain C and (resid 114 or (resid 115 and (name...CB114 - 231115 - 232
213ALAALAASPASP(chain E and resid 114 through 228)EA114 - 228115 - 229

NCS ensembles :
ID
1
2
3

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Components

#1: Protein Protein TOC75, chloroplastic / 75 kDa chloroplast outer envelope protein / 75 kDa translocon at the outer-envelope membrane of ...75 kDa chloroplast outer envelope protein / 75 kDa translocon at the outer-envelope membrane of chloroplasts / Import intermediate-associated protein of 75 kDa


Mass: 35527.430 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Pisum sativum (garden pea) / Gene: TOC75, IAP75, OEP75 / Production host: Escherichia coli (E. coli) / References: UniProt: Q43715
#2: Antibody fabax9 Heavy Chain


Mass: 26466.283 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli)
#3: Antibody fabax9 Light Chain


Mass: 23596.197 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli)
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 422 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 1.79 Å3/Da / Density % sol: 31.2 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: 0.1M Sodium Hepes pH 7, 18% PEG 12,000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.03 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 5, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.03 Å / Relative weight: 1
ReflectionResolution: 2→50 Å / Num. obs: 69080 / % possible obs: 85.9 % / Redundancy: 3 % / Biso Wilson estimate: 31.7 Å2 / CC1/2: 0.992 / CC star: 0.998 / Rpim(I) all: 0.082 / Rrim(I) all: 0.148 / Net I/σ(I): 11.58
Reflection shellResolution: 2→2.07 Å / Redundancy: 3 % / Num. unique obs: 7224 / CC1/2: 0.275 / CC star: 0.657 / Rpim(I) all: 0.668 / % possible all: 88.6

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassificationNB
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
PHENIX1.20_4459refinement
PDB_EXTRACT3.27data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5BJZ, 5UAY

5bjz

5uay


Resolution: 2→41.66 Å / SU ML: 0.3 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 27.84 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.259 1953 2.83 %
Rwork0.2109 66963 -
obs0.2122 68916 85.45 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 150.5 Å2 / Biso mean: 41.3612 Å2 / Biso min: 12 Å2
Refinement stepCycle: final / Resolution: 2→41.66 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8460 0 0 422 8882
Biso mean---39.64 -
Num. residues----1106
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A1893X-RAY DIFFRACTION12.88TORSIONAL
12D1893X-RAY DIFFRACTION12.88TORSIONAL
21B1853X-RAY DIFFRACTION12.88TORSIONAL
22H1853X-RAY DIFFRACTION12.88TORSIONAL
31C1027X-RAY DIFFRACTION12.88TORSIONAL
32E1027X-RAY DIFFRACTION12.88TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2-2.050.33281450.32084848499385
2.05-2.10.32831260.30164845497188
2.1-2.160.30951550.28264846500185
2.16-2.230.29491110.26454487459881
2.23-2.310.32151430.27374549469281
2.31-2.410.31441410.25465093523490
2.41-2.510.29591450.25094941508689
2.51-2.650.31531540.25184903505788
2.65-2.810.34561550.24614803495886
2.81-3.030.26491360.23264654479083
3.03-3.340.27191270.21944341446877
3.34-3.820.24511500.1874720487085
3.82-4.810.18411300.15284841497186
4.81-41.660.20271350.16385092522791
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.0847-0.0432-0.0540.02280.02950.02240.05270.054-0.00880.06830.0398-0.08130.3034-0.20730.00010.2818-0.046-0.06340.40090.02340.3161-45.1322-7.372429.3872
20.03040.0184-0.02050.0242-0.05070.0379-0.10130.48490.0885-0.09490.2489-0.1247-0.08190.0977-0.03830.1478-0.040.1260.9203-0.02810.3894-26.9929-10.560715.6431
30.19260.16370.15520.2580.08740.412-0.1316-0.0764-0.119-0.14760.14480.1559-0.20420.5290.03050.2480.0794-0.05030.54730.00740.2251-32.6744-11.990525.904
40.1084-0.0632-0.24220.2316-0.04360.775-0.1186-0.08920.0789-0.1848-0.53690.64060.23260.0165-0.05170.14470.078-0.04180.4179-0.23120.4912-54.2471-6.279226.8748
50.1342-0.01420.31090.1095-0.02860.28190.05960.1459-0.046-0.3333-0.33450.3484-0.22250.2672-0.04590.33980.0599-0.08750.2765-0.08770.2994-46.08651.397422.9118
60.04030.00550.02520.0195-0.00580.06720.1865-0.2752-0.08890.1339-0.02120.2324-0.0312-0.0314-00.38330.007-0.02990.3135-0.01410.2343-40.5359-0.115427.0283
70.126-0.0371-0.01290.0272-0.00690.149-0.27830.1617-0.4045-0.03130.3502-0.2336-0.22120.0144-0.00020.30430.0711-0.0180.2362-0.03090.3207-19.7802-20.04-124.9982
80.0260.0007-0.01270.0048-0.00110.01010.1191-0.0051-0.054-0.0229-0.02390.01380.0776-0.114-0.00010.96480.07720.20660.7727-0.15420.8176-21.6833-8.719-114.4065
90.18260.0513-0.1760.0702-0.12920.3696-0.1306-0.18350.27820.4077-0.26980.3593-0.54060.1054-0.00970.62210.0559-0.01490.275-0.02010.4125-36.7881-7.2916-106.6475
100.03280.03290.00070.1992-0.12510.13730.26590.31830.14850.005-0.08090.0289-0.3697-0.41490.06010.29720.15410.00030.31070.04050.2886-34.3644-12.8776-119.1115
110.17090.0056-0.17720.02770.00280.0374-0.08940.2064-0.06960.22480.17410.12880.19050.22970.00030.28440.04760.01950.28720.05820.3037-16.9535-30.0891-123.1594
12-0.01110.0054-0.05530.3760.07971.037-0.0183-0.5346-0.307-0.03010.6421-0.241-0.04970.64390.94220.05270.2605-0.1230.39840.21240.2437-10.4145-26.334-117.4791
130.1871-0.29220.21740.4999-0.28460.2581-0.18920.3970.21490.02640.0594-0.0458-0.0888-0.0781-00.2543-0.0083-0.06210.24110.0110.2734-14.7022-19.4047-121.4495
140.53620.5438-0.48040.69060.5691.0543-0.0254-0.1435-0.01580.11390.0149-0.0091-0.0228-0.2034-0.00090.12430.02390.00760.22090.02060.2371-49.7619-27.71316.7502
15-0.1018-0.213-0.09370.6962-0.41371.5492-0.04410.01650.12380.0897-0.01390.00630.42370.0269-0.31570.0905-0.0260.03910.2154-0.02010.1738-44.6295-30.3036-2.9698
160.8294-0.11160.11950.87480.42080.45290.10730.30120.22880.3893-0.3808-0.24910.9656-0.3305-0.42470.6362-0.11850.020.122-0.09030.1513-45.54-47.2883-20.0115
170.62710.627-0.5550.71170.23351.11090.08610.02620.0045-0.0507-0.02560.09560.12520.1038-0.00360.19520.05040.01510.1468-0.03280.2716-40.6747-35.2134-102.1654
180.50330.02840.3183-0.1710.33360.9229-0.05730.1240.08860.04050.012-0.01950.1808-0.1196-0.1740.21770.03630.05490.032-0.03680.2425-43.6287-30.0418-93.8858
190.5860.0274-0.81870.5185-0.31870.5831-0.44820.08740.21320.03170.1948-0.07430.2518-0.6136-0.10430.2882-0.10030.02490.4208-0.03840.2283-60.3526-35.4014-75.5046
200.67360.0507-0.8560.4512-0.60271.93590.3941-0.3403-0.06150.2852-0.6492-0.2801-1.03720.9565-0.1080.1891-0.2138-0.01070.47790.04090.2955-30.8708-18.3434-80.8326
210.2218-0.07260.1830.25030.19740.56870.2636-0.0827-0.0641-0.0189-0.24110.1778-0.29950.20660.00040.2172-0.0433-0.0060.1910.00840.2122-35.1183-16.8474-91.5311
220.07670.0143-0.03850.1441-0.04060.03290.0235-0.35550.21660.10760.022-0.1846-0.46360.4892-0.02740.265-0.18870.01420.4021-0.00560.2713-28.2455-12.2024-89.5473
230.00720.0773-0.02070.383-0.45140.84080.2023-0.0601-0.09830.2422-0.47430.0226-0.50540.6245-0.10940.2434-0.02430.00940.2362-0.03020.2507-34.1107-21.1531-91.1019
240.03870.0646-0.00380.07850.03910.05490.1928-0.1309-0.31760.45-0.2378-0.2349-0.18630.2102-0.00190.3061-0.04490.08190.2819-0.03910.2053-41.4388-18.819-69.9308
251.3184-0.72580.35131.33460.03880.4869-0.2825-0.4040.0083-0.36360.1099-0.49280.4151-0.5468-0.15990.4081-0.18850.08580.48940.08480.0674-58.1131-40.2871-64.1205
260.10220.03270.02540.22270.270.2514-0.2369-0.55360.183-0.16350.082-0.38830.26890.38990.00340.29040.03640.01180.50410.08530.2912-45.5798-34.0515-59.3505
270.32680.3643-0.1950.25280.1570.4076-0.1355-0.2799-0.1140.0575-0.0723-0.25570.10780.08220.0020.34760.03370.04490.50390.08440.2593-49.9486-37.0418-63.8832
280.15490.2527-0.1050.3630.03160.11080.0344-0.2068-0.0840.3130.1831-0.13980.21810.30160.02420.3129-0.13050.0270.66480.01590.2161-52.8276-32.7243-54.1881
290.50080.260.03230.56770.08230.5004-0.0860.14830.2372-0.42110.30880.0993-0.85790.39460.16990.479-0.13260.03440.2583-0.03430.2003-36.2837-13.9935-14.1168
300.2689-0.08370.4416-0.0109-0.05130.7821-0.17910.1294-0.2556-0.0849-0.0530.0095-0.870.1249-0.11160.3826-0.01420.05610.1773-0.01710.2058-37.5322-12.1474-4.3706
310.13190.03450.19030.5299-0.20050.3812-0.151-0.1342-0.3049-0.34260.202-0.02230.0020.47910.05820.0974-0.05940.01390.3511-0.0190.2329-30.8817-22.7884-3.2183
320.1931-0.0127-0.08440.4960.28460.1542-0.0357-0.48670.2256-0.0345-0.27040.0967-0.78960.1888-0.25320.4074-0.84540.1308-0.796-0.00050.3225-31.1599-10.0218-5.6615
330.25370.00320.1983-0.00010.02330.13320.0981-0.06560.041-0.12860.2449-0.1199-0.53210.66470.03320.1686-0.08160.04650.2437-0.04180.2533-33.7029-20.9457-10.214
34-0.02680.09240.07010.08920.12460.1763-0.2821-0.07930.1733-0.1085-0.1065-0.0148-0.2355-0.4791-0.00010.33990.0184-0.04760.1538-0.02590.2716-44.9653-13.86334.4867
350.1537-0.08290.01660.0358-0.02970.1188-0.03110.05090.0136-0.13230.04670.0771-0.04950.33670.00730.3844-0.09610.04790.23170.00070.2055-36.8337-30.2014-28.2261
361.9036-0.85310.2673.0906-0.62940.62170.0594-0.15090.34750.0124-0.4632-0.29690.207-0.7042-0.32210.4219-0.18810.00560.4728-0.1427-0.0068-52.8771-47.245-30.1015
370.5256-0.2097-0.35810.48710.36230.34160.23270.48310.2205-0.5723-0.35670.1948-0.4184-0.27720.00480.67650.0457-0.0676-0.02550.02610.3574-44.9632-31.0701-33.7799
380.23930.24540.07540.16030.10380.1540.06350.07350.4323-0.1077-0.38690.451-0.0875-0.2166-0.01040.47280.0982-0.13460.3355-0.110.3196-55.298-34.7467-34.7823
390.02440.01460.00790.00990.0071-0.00120.3321-0.3766-0.32790.48040.0417-0.06870.77010.1581-0.00010.40520.0460.05330.21690.02880.2625-35.0717-31.6651-22.4907
400.1668-0.2190.02590.25060.0118-0.037-0.2437-0.25820.359-0.3774-0.22410.20570.3532-0.6194-0.05390.4186-0.1445-0.05270.5159-0.08890.2407-58.166-43.4173-35.0194
410.376-0.2358-0.26610.15270.17380.22610.01930.1627-0.061-0.2723-0.0643-0.0026-0.1913-0.3371-0.03140.6847-0.0467-0.02780.29390.02820.2314-46.3372-39.3578-40.8753
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'E' and (resid 113 through 121 )E113 - 121
2X-RAY DIFFRACTION2chain 'E' and (resid 122 through 138 )E122 - 138
3X-RAY DIFFRACTION3chain 'E' and (resid 139 through 166 )E139 - 166
4X-RAY DIFFRACTION4chain 'E' and (resid 167 through 184 )E167 - 184
5X-RAY DIFFRACTION5chain 'E' and (resid 185 through 218 )E185 - 218
6X-RAY DIFFRACTION6chain 'E' and (resid 219 through 228 )E219 - 228
7X-RAY DIFFRACTION7chain 'C' and (resid 114 through 126 )C114 - 126
8X-RAY DIFFRACTION8chain 'C' and (resid 127 through 131 )C127 - 131
9X-RAY DIFFRACTION9chain 'C' and (resid 132 through 138 )C132 - 138
10X-RAY DIFFRACTION10chain 'C' and (resid 139 through 159 )C139 - 159
11X-RAY DIFFRACTION11chain 'C' and (resid 160 through 184 )C160 - 184
12X-RAY DIFFRACTION12chain 'C' and (resid 185 through 202 )C185 - 202
13X-RAY DIFFRACTION13chain 'C' and (resid 203 through 231 )C203 - 231
14X-RAY DIFFRACTION14chain 'D' and (resid 5 through 102 )D5 - 102
15X-RAY DIFFRACTION15chain 'D' and (resid 103 through 150 )D103 - 150
16X-RAY DIFFRACTION16chain 'D' and (resid 151 through 232 )D151 - 232
17X-RAY DIFFRACTION17chain 'A' and (resid 4 through 94 )A4 - 94
18X-RAY DIFFRACTION18chain 'A' and (resid 95 through 150 )A95 - 150
19X-RAY DIFFRACTION19chain 'A' and (resid 151 through 231 )A151 - 231
20X-RAY DIFFRACTION20chain 'B' and (resid 3 through 19 )B3 - 19
21X-RAY DIFFRACTION21chain 'B' and (resid 20 through 62 )B20 - 62
22X-RAY DIFFRACTION22chain 'B' and (resid 63 through 76 )B63 - 76
23X-RAY DIFFRACTION23chain 'B' and (resid 77 through 104 )B77 - 104
24X-RAY DIFFRACTION24chain 'B' and (resid 105 through 116 )B105 - 116
25X-RAY DIFFRACTION25chain 'B' and (resid 117 through 140 )B117 - 140
26X-RAY DIFFRACTION26chain 'B' and (resid 141 through 158 )B141 - 158
27X-RAY DIFFRACTION27chain 'B' and (resid 159 through 200 )B159 - 200
28X-RAY DIFFRACTION28chain 'B' and (resid 201 through 216 )B201 - 216
29X-RAY DIFFRACTION29chain 'H' and (resid 3 through 19 )H3 - 19
30X-RAY DIFFRACTION30chain 'H' and (resid 20 through 39 )H20 - 39
31X-RAY DIFFRACTION31chain 'H' and (resid 40 through 62 )H40 - 62
32X-RAY DIFFRACTION32chain 'H' and (resid 63 through 76 )H63 - 76
33X-RAY DIFFRACTION33chain 'H' and (resid 77 through 92 )H77 - 92
34X-RAY DIFFRACTION34chain 'H' and (resid 93 through 104 )H93 - 104
35X-RAY DIFFRACTION35chain 'H' and (resid 105 through 121 )H105 - 121
36X-RAY DIFFRACTION36chain 'H' and (resid 122 through 140 )H122 - 140
37X-RAY DIFFRACTION37chain 'H' and (resid 141 through 153 )H141 - 153
38X-RAY DIFFRACTION38chain 'H' and (resid 154 through 166 )H154 - 166
39X-RAY DIFFRACTION39chain 'H' and (resid 167 through 177 )H167 - 177
40X-RAY DIFFRACTION40chain 'H' and (resid 178 through 193 )H178 - 193
41X-RAY DIFFRACTION41chain 'H' and (resid 194 through 217 )H194 - 217

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