0.5 mM [U-100% 13C; U-100% 15N] Calmodulin, 0.85 mM Cyclic nucleotide-gated cation channel beta-1, 20 mM [U-100% 2H] TRIS, 1 mM Calcium chloride, 90% H2O/10% D2O
13C_15N_Sample_H2O
90% H2O/10% D2O
solution
2
0.5 mM [U-100% 13C; U-100% 15N] Calmodulin, 0.85 mM Cyclic nucleotide-gated cation channel beta-1, 20 mM [U-100% 2H] TRIS, 1 mM Calcium chloride, 100% D2O
13C_15N_Sample_D2O
100% D2O
solution
3
0.5 mM [U-10% 13C; U-100% 15N] Calmodulin, 0.85 mM Cyclic nucleotide-gated cation channel beta-1, 20 mM [U-100% 2H] TRIS, 1 mM Calcium chloride, 100% D2O
10%13C_15N_Sample_D2O
100% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
0.5mM
Calmodulin
[U-100% 13C; U-100% 15N]
1
0.85mM
Cyclic nucleotide-gated cation channel beta-1
naturalabundance
1
20mM
TRIS
[U-100% 2H]
1
1mM
Calciumchloride
naturalabundance
1
0.5mM
Calmodulin
[U-100% 13C; U-100% 15N]
2
0.85mM
Cyclic nucleotide-gated cation channel beta-1
naturalabundance
2
20mM
TRIS
[U-100% 2H]
2
1mM
Calciumchloride
naturalabundance
2
0.5mM
Calmodulin
[U-10% 13C; U-100% 15N]
3
0.85mM
Cyclic nucleotide-gated cation channel beta-1
naturalabundance
3
20mM
TRIS
[U-100% 2H]
3
1mM
Calciumchloride
naturalabundance
3
試料状態
イオン強度: 1 mM / Label: Condition_1 / pH: 7 / 圧: 1 atm / 温度: 308 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker AVANCE III
Bruker
AVANCEIII
600
1
Bruker AVANCE III
Bruker
AVANCEIII
800
2
-
解析
NMR software
名称
開発者
分類
HADDOCK
Bonvin
精密化
HADDOCK
Bonvin
structurecalculation
Sparky
Goddard
chemicalshiftassignment
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
解析
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: The authors state that for the structure of the calmodulin N-lobe, they used residues 4-81 from the available structure of the calmodulin/IQ domain complex (PDB ID - 2F3Y, chain A). The ...詳細: The authors state that for the structure of the calmodulin N-lobe, they used residues 4-81 from the available structure of the calmodulin/IQ domain complex (PDB ID - 2F3Y, chain A). The structure of the peptide fragment of the Cyclic nucleotide-gated cation channel beta-1 was generated in Modeller 9.25 using the template of the peptide of cyclic-nucleotide-gated olfactory channel (PDB ID - 1SY9, chain B). Finally, the HADDOCK calculation was performed on an energy minimized peptide obtained after energy minimization in Gromacs.