Details | Type | Solution-ID | Contents | Label | Solvent system | Details |
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solution1 | 500 uM [U-100% 15N] 5_SL4 RNA, 95% H2O/5% D2O | 15N_sample95% H2O/5% D2O | solution2 | 500 uM [U-13C; U-15N]-Ura,Gua 5_SL4 RNA, 95% H2O/5% D2O | G,U-13C,15N_sample | 95% H2O/5% D2O | solution3 | 500 uM [U-13C; U-15N]-Ade,Cyt 5_SL4 RNA, 95% H2O/5% D2O | A,C-13C,15N_sample | 95% H2O/5% D2O | solution4 | 350 uM [U-13C; U-15N] 5_SL4sh RNA, 95% H2O/5% D2O | 5_SL4sh 13C,15N_sample95% H2O/5% D2Osequence GGCACUCGGCUGCAUGCUUAGUGCCsolution9 | 1.8 mM 5_SL4sh RNA, 95% H2O/5% D2O5_SL4sh unlabeled_sample95% H2O/5% D2Osequence GGCACUCGGCUGCAUGCUUAGUGCCsolution5 | 200 uM [U-13C; U-15N]-Ade,Cyt 5_SL4 RNA, 11.6 mg/mL unlabeled Pf1 phage, 95% H2O/5% D2O | 5_SL4 A,C 13C,15N RDC95% H2O/5% D2O | solution7 | 200 uM [U-13C; U-15N]-Gua,Ury 5_SL4 RNA, 11.6 uM unlabeled Pf1 phage, 95% H2O/5% D2O | 5_SL4 G,U 13C,15N RDC95% H2O/5% D2O | solution6 | 200 uM [U-13C; U-15N]-Ade,Cyt 5_SL4 RNA, 95% H2O/5% D2O | 5_SL4 A,C 13C,15N RDC reference95% H2O/5% D2O | solution8 | 200 uM [U-13C; U-15N]-Gua,Ura 5_SL4 RNA, 95% H2O/5% D2O | 5_SL4 G,U 13C,15N RDC reference95% H2O/5% D2O | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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Sample | Conc. (mg/ml) | Component | Isotopic labeling | Solution-ID |
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500 uM5_SL4 RNA[U-100% 15N] | 1 | 500 uM5_SL4 RNA[U-13C; U-15N]-Ura,Gua | 2 | 500 uM5_SL4 RNA[U-13C; U-15N]-Ade,Cyt | 3 | 350 uM5_SL4sh RNA[U-13C; U-15N] | 4 | 1.8 mM5_SL4sh RNAnatural abundance9 | 200 uM5_SL4 RNA[U-13C; U-15N]-Ade,Cyt | 5 | 11.6 mg/mLPf1 phageunlabeled5 | 200 uM5_SL4 RNA[U-13C; U-15N]-Gua,Ury | 7 | 11.6 uMPf1 phageunlabeled7 | 200 uM5_SL4 RNA[U-13C; U-15N]-Ade,Cyt | 6 | 200 uM5_SL4 RNA[U-13C; U-15N]-Gua,Ura | 8 | | | | | | | | | | | | | | | | | | | | | | | | | |
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Sample conditions | Details: 25 mM potassium phosphate buffer 50 mM potassium chloride / Ionic strength: 100 mM / Label: conditions_1 / pH: 6.2 / PH err: 0.05 / Pressure: 1 bar Conditions-ID | Temperature (K) |
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1 | 283 K2 | 298 K | |
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