PDB-8coi: Human adenovirus-derived synthetic ADDobody binder

Basic information

• Entry: Database: PDB / ID: 8coi
• Title: Human adenovirus-derived synthetic ADDobody binder
• Components: ADDobody
• Keywords: VIRAL PROTEIN / penton base / crown domain / human adenovirus
• Biological species: Human adenovirus sp.
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.17 Å
• Authors: Buzas, D. / Toelzer, C. / Gupta, K. / Berger-Schaffitzel, C. / Berger, I.

Funding support

United Kingdom, 1items

Organization	Grant number	Country
Max Planck Bristol Centre for Minimal Biology - University of Bristol	U100572-102	United Kingdom

• Citation: Journal: Structure / Year: 2024; Title: Engineering the ADDobody protein scaffold for generation of high-avidity ADDomer super-binders.; Authors: Dora Buzas / Huan Sun / Christine Toelzer / Sathish K N Yadav / Ufuk Borucu / Gunjan Gautam / Kapil Gupta / Joshua C Bufton / Julien Capin / Richard B Sessions / Frederic Garzoni / Imre Berger / Christiane Schaffitzel / ; Abstract: Adenovirus-derived nanoparticles (ADDomer) comprise 60 copies of adenovirus penton base protein (PBP). ADDomer is thermostable, rendering the storage, transport, and deployment of ADDomer-based therapeutics independent of a cold chain. To expand the scope of ADDomers for new applications, we engineered ADDobodies, representing PBP crown domain, genetically separated from PBP multimerization domain. We inserted heterologous sequences into hyper-variable loops, resulting in monomeric, thermostable ADDobodies expressed at high yields in Escherichia coli. The X-ray structure of an ADDobody prototype validated our design. ADDobodies can be used in ribosome display experiments to select a specific binder against a target, with an enrichment factor of ∼10-fold per round. ADDobodies can be re-converted into ADDomers by genetically reconnecting the selected ADDobody with the PBP multimerization domain from a different species, giving rise to a multivalent nanoparticle, called Chimera, confirmed by a 2.2 Å electron cryo-microscopy structure. Chimera comprises 60 binding sites, resulting in ultra-high, picomolar avidity to the target.

History

Deposition	Feb 28, 2023	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Dec 27, 2023	Provider: repository / Type: Initial release
Revision 1.1	Jan 24, 2024	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Mar 20, 2024	Group: Database references / Category: citation / Item: _citation.journal_volume / _citation.page_first

Assembly

Deposited unit

A: ADDobody

B: ADDobody

C: ADDobody

D: ADDobody

E: ADDobody

F: ADDobody

G: ADDobody

H: ADDobody

I: ADDobody

J: ADDobody

K: ADDobody

L: ADDobody

M: ADDobody

N: ADDobody

O: ADDobody

P: ADDobody

Q: ADDobody

R: ADDobody

S: ADDobody

T: ADDobody

Summary:

• 705 kDa, 20 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	704,970	20
Polymers	704,970	20
Non-polymers	0	0
Water	0	0

1

A: ADDobody

Summary:

• defined by author
• Evidence: gel filtration
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

2

B: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

3

C: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

4

D: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

5

E: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

6

F: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

7

G: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

8

H: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

9

I: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

10

J: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

11

K: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

12

L: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

13

M: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

14

N: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

15

O: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

16

P: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

17

Q: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

18

R: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

19

S: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

20

T: ADDobody

Summary:

• defined by author
• 35.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,249	1
Polymers	35,249	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Unit cell

Length a, b, c (Å)	103.853, 104.629, 180.494
Angle α, β, γ (deg.)	92.03, 95.65, 112.61
Int Tables number	1
Space group name H-M	P1

Components

#1: Protein

A B C D E F G H I J K L M N O P Q R S T
ADDobody

Details:

Mass: 35248.512 Da / Num. of mol.: 20
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human adenovirus sp.
Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 3.22 ų/Da / Density % sol: 61.82 %
• Crystal grow: Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.5 / Details: 20 w/v % PEG 3350, 0.15 M citrate pH 5.5

Data collection

• Diffraction: Mean temperature: 100 K / Serial crystal experiment: N
• Diffraction source: Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9795 Å
• Detector: Type: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Oct 21, 2019
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.9795 Å / Relative weight: 1
• Reflection: Resolution: 3.17→96.41 Å / Num. obs: 116474 / % possible obs: 98.6 % / Redundancy: 3.6 % / R_merge(I) obs: 0.12 / Net I/σ(I): 8.88
• Reflection shell: Resolution: 3.17→3.28 Å / Num. unique obs: 11604 / CC_1/2: 0.723

Processing

Software

Name	Version	Classification
PHENIX	(1.19.2_4158: ???)	refinement
XDS		data reduction
Coot		model building
PHASER		phasing
Aimless		data scaling

Refinement

Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.17→19.97 Å / SU ML: 0.44 / Cross valid method: FREE R-VALUE / σ(F): 1.93 / Phase error: 27.16 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.2613	5851	5.02 %
Rwork	0.213	-	-
obs	0.2154	116437	98.6 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 3.17→19.97 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	37335	0	0	0	37335

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.002	38245
X-RAY DIFFRACTION	f_angle_d	0.491	51997
X-RAY DIFFRACTION	f_dihedral_angle_d	13.002	13760
X-RAY DIFFRACTION	f_chiral_restr	0.042	5665
X-RAY DIFFRACTION	f_plane_restr	0.004	6792

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
3.17-3.2	0.3333	197	0.3092	3587	X-RAY DIFFRACTION	97
3.2-3.24	0.3317	209	0.2983	3669	X-RAY DIFFRACTION	99
3.24-3.28	0.3551	176	0.2937	3703	X-RAY DIFFRACTION	98
3.28-3.32	0.3302	190	0.2906	3675	X-RAY DIFFRACTION	99
3.32-3.37	0.3316	183	0.2877	3698	X-RAY DIFFRACTION	98
3.37-3.41	0.3192	201	0.2786	3687	X-RAY DIFFRACTION	99
3.41-3.46	0.3383	181	0.2758	3719	X-RAY DIFFRACTION	99
3.46-3.51	0.2917	178	0.2632	3662	X-RAY DIFFRACTION	99
3.51-3.57	0.2997	204	0.2548	3661	X-RAY DIFFRACTION	99
3.57-3.63	0.2959	185	0.2509	3704	X-RAY DIFFRACTION	99
3.63-3.69	0.3047	187	0.2501	3700	X-RAY DIFFRACTION	99
3.69-3.75	0.3112	215	0.2487	3682	X-RAY DIFFRACTION	99
3.75-3.83	0.2999	182	0.2333	3694	X-RAY DIFFRACTION	99
3.83-3.9	0.2694	187	0.2214	3712	X-RAY DIFFRACTION	99
3.9-3.99	0.2395	187	0.2038	3717	X-RAY DIFFRACTION	99
3.99-4.08	0.2557	191	0.1928	3647	X-RAY DIFFRACTION	99
4.08-4.18	0.2329	197	0.1913	3741	X-RAY DIFFRACTION	99
4.18-4.29	0.245	161	0.2001	3707	X-RAY DIFFRACTION	99
4.29-4.42	0.2614	203	0.1943	3702	X-RAY DIFFRACTION	99
4.42-4.56	0.229	222	0.1811	3659	X-RAY DIFFRACTION	99
4.56-4.72	0.2222	190	0.1746	3749	X-RAY DIFFRACTION	99
4.72-4.91	0.2211	186	0.1716	3647	X-RAY DIFFRACTION	99
4.91-5.12	0.2437	167	0.1818	3766	X-RAY DIFFRACTION	99
5.13-5.39	0.2379	199	0.1942	3679	X-RAY DIFFRACTION	99
5.39-5.72	0.2678	197	0.2025	3717	X-RAY DIFFRACTION	99
5.72-6.15	0.2474	198	0.2105	3696	X-RAY DIFFRACTION	99
6.15-6.75	0.2688	227	0.2256	3666	X-RAY DIFFRACTION	99
6.75-7.67	0.2626	242	0.2168	3646	X-RAY DIFFRACTION	99
7.68-9.49	0.2087	212	0.1816	3691	X-RAY DIFFRACTION	99
9.49-19.97	0.2501	197	0.1893	3607	X-RAY DIFFRACTION	97

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	5.6028	-4.8105	-3.2339	4.1277	2.8862	2.8309	-0.5904	-0.6254	3.0289	-1.7337	0.015	-0.7876	-1.9193	-0.9198	0.4328	1.1478	0.0984	-0.2221	0.9737	-0.0123	1.3092	-20.9196	6.5922	-2.1353
2	6.4543	-3.3491	-0.7683	4.5685	2.0066	3.558	0.8374	0.2461	0.051	-0.8854	-0.1715	-0.4333	-0.1183	0.4704	-0.4347	0.6282	0.0837	0.1299	0.7113	-0.0263	0.557	-0.3497	-7.4833	-13.5606
3	2.3498	1.4854	2.0914	2.234	0.1896	2.4337	-0.0922	0.2403	-0.1006	-0.214	0.1842	-0.2018	0.013	0.2362	-0.0867	0.412	0.176	0.2067	0.7865	-0.0103	0.611	2.2932	-4.0757	1.17
4	3.2492	0.2721	1.3938	4.3988	-1.0828	4.0556	-0.0542	0.378	0.3528	-0.3457	-0.2193	-0.3973	-0.0818	0.2953	0.1989	0.4256	0.1444	0.1362	0.7054	0.0614	0.5585	0.6731	7.4296	-4.8514
5	0.2441	0.1987	-0.4613	1.2892	1.2696	1.6182	0.0183	0.481	-0.3556	0.0917	-0.1437	-0.3055	0.4936	0.6192	0.094	0.5458	0.2642	0.1891	1.0325	-0.0536	1.0348	13.5808	-5.6668	1.9711
6	2.8411	2.0808	-0.7428	5.9191	3.6156	3.9431	-0.2117	0.1437	-0.2571	-1.0495	-0.6795	1.3783	-1.5203	0.1941	0.576	0.3774	-0.0106	0.0858	0.7561	0.0426	0.5628	2.3176	11.2782	0.027
7	4.7279	4.7812	2.6794	8.9346	3.476	9.926	-0.2085	-0.6919	-0.3365	1.1285	-0.432	0.3416	2.2256	-0.4775	0.0716	0.6326	0.2334	0.2197	0.6817	0.008	0.7974	-1.7544	-8.3926	5.8718
8	4.3211	-0.6904	-0.195	4.0879	-1.7915	2.8125	-0.3013	-0.1763	0.0754	0.5056	-0.4725	-0.7896	-0.0382	0.8613	0.6508	0.4802	-0.1185	-0.123	0.4804	-0.0226	0.6521	-10.0914	36.0751	-8.5064
9	1.9168	1.1205	-0.8061	6.7912	-5.3461	4.1812	-0.8779	0.7788	0.7152	-1.4876	1.6337	2.4508	-1.336	0.4191	-0.0861	1.0287	-0.2321	-0.1408	0.9672	0.0818	0.7851	-18.3617	54.6827	-37.65
10	2.8287	0.8513	2.4535	3.0842	-1.8285	7.7397	0.337	-0.1307	0.1699	-0.0711	0.1505	-0.3356	0.1059	0.7043	-0.3241	0.3739	0.0491	0.0721	0.6083	-0.0113	0.5232	-14.3838	43.2462	-13.6842
11	4.5394	1.3037	-0.3782	3.9521	-2.222	1.9674	0.0071	0.1123	-0.3702	-0.3603	-0.0292	-0.059	-0.388	-0.22	0.1808	0.4632	0.0132	0.0217	0.5812	-0.0829	0.3791	-26.257	44.0829	-17.3169
12	6.853	-2.4483	-0.79	4.1868	-0.8749	6.6882	-0.2843	-0.481	-0.2089	-0.0042	0.4337	-0.1265	-0.1957	-0.466	-0.4503	0.2995	-0.0248	-0.0115	0.4672	-0.032	0.4973	-36.5539	40.2525	-12.9749
13	9.458	-0.3674	-0.1446	3.4868	0.2524	4.4953	-0.0336	0.3908	-0.8964	-0.1961	-0.0949	0.0918	0.7497	0.1691	0.2248	0.3867	-0.0197	0.0722	0.5854	0.0053	0.5308	-17.3784	31.1438	-12.2974
14	3.4473	-0.9967	2.1181	4.48	-6.1211	8.1565	0.2558	0.4325	-0.8243	-0.6392	-0.6549	-0.1553	1.21	-0.1349	0.3904	0.5685	-0.0816	0.1435	0.7711	-0.141	0.8003	-18.5245	24.0877	-17.5875
15	8.4736	-0.8399	1.5609	4.2268	-1.0911	5.9643	-0.238	0.2525	1.318	-0.2807	0.1586	0.5748	-0.532	-1.0548	0.1911	0.4642	0.0715	-0.06	0.697	0.0923	0.5576	-38.5908	48.9875	-21.2997
16	3.9773	0.7862	-0.2804	8.4963	-1.6054	3.1163	0.2579	0.7908	-0.0206	-1.0472	-0.1656	0.722	0.1169	0.1025	-0.1206	0.3852	0.1115	-0.0687	0.5421	-0.074	0.3861	-31.9015	37.647	-20.6022
17	2.8553	1.56	0.8743	7.9057	-0.6024	3.4775	0.0881	-0.3097	-0.0912	0.3645	-0.506	-0.1379	-0.1331	-0.4189	0.3069	0.2042	0.1039	0.0683	0.7181	0.073	0.5488	-26.4425	37.7758	-10.8008
18	3.8253	0.9582	0.8539	3.8578	5.2216	7.8918	0.0632	-0.1351	-0.1319	-1.3988	-0.1379	0.2684	-0.9516	-0.3746	0.2013	0.46	0.0474	-0.084	0.5592	0.1468	0.5957	-42.9081	-15.0676	8.1593
19	2.111	0.227	-0.9649	2.4846	-0.4055	1.9826	0.1602	0.0345	0.0699	-0.3237	0.0308	0.185	0.2235	-0.1455	-0.2182	0.4147	-0.0106	-0.0545	0.4837	-0.0705	0.4734	-32.0707	-17.102	6.085
20	3.7434	1.1165	-1.094	2.8751	-0.8099	2.7903	0.0173	0.5232	0.0033	-0.4663	0.0237	-0.0914	0.3005	-0.0811	-0.0402	0.4429	-0.0225	-0.0071	0.6143	-0.1014	0.4723	-28.9438	-13.4659	-2.1978
21	2.5824	2.8683	0.6282	6.7141	-1.6362	7.162	0.9692	-0.6426	0.2908	0.9289	-0.3441	-0.7974	0.0184	0.5349	-0.5973	0.4466	-0.0109	0.1756	0.5818	-0.1315	0.6545	-27.2859	-13.8652	12.9357
22	7.7605	5.0063	-2.5189	6.2785	-0.9797	3.1887	0.1448	0.151	-0.5971	0.8451	-0.1614	-0.5257	0.1213	0.273	0.1454	0.4765	0.0746	-0.1198	0.5866	-0.0911	0.669	1.2797	57.4613	21.3629
23	2.0419	0.4265	0.3771	1.57	0.1037	3.1545	0.0701	-0.121	0.0141	0.1604	-0.0619	-0.1998	0.0863	0.3744	-0.0671	0.3044	-0.0297	0.0224	0.4714	0.0076	0.5638	-2.8588	54.0446	13.228
24	2.2073	0.4177	0.3671	1.4503	1.6228	5.1172	0.1057	0.1849	0.1084	0.1249	-0.0185	-0.0509	-0.3421	0.2958	-0.043	0.375	-0.0622	0.0707	0.5149	0.0978	0.5845	-5.2625	57.324	2.5757
25	6.306	3.5172	0.9715	3.7619	4.1372	8.0216	0.8437	-1.0095	0.1761	1.1093	-0.8027	-0.285	0.4553	-0.1784	-0.2633	0.3431	0.0377	0.0033	0.4436	0.1141	0.5808	-14.2738	58.2685	10.8196
26	5.3556	3.8515	3.2263	3.75	-0.9613	6.4215	-0.1743	0.4228	0.1059	-0.1708	0.0793	-0.1884	-0.5154	0.0918	0.0454	0.405	0.0873	0.1259	0.743	0.0612	0.7736	16.7225	12.5575	18.7842
27	1.6545	0.7374	-0.8746	1.3556	-1.1121	2.8473	0.0308	0.0375	0.0431	0.4588	-0.1287	-0.6209	-0.1204	0.2932	0.0765	0.4157	0.0616	-0.1767	0.7011	0.0941	0.7954	12.533	11.729	35.2448
28	8.6996	6.9874	-3.8661	9.2124	-3.0625	4.6614	0.3996	0.4886	1.176	0.7984	0.1187	0.4166	-0.5744	0.5523	-0.4634	0.5974	0.0484	0.0012	0.9321	-0.0084	0.7459	-38.1224	4.6858	46.6452
29	3.6911	2.0488	0.7345	6.1533	0.5655	1.9566	0.0272	-0.7195	0.375	0.2524	-0.1362	1.3685	0.4159	-0.5376	0.0586	0.5785	-0.0631	0.2211	0.9376	0.0064	0.4737	-42.0991	-9.0461	50.4743
30	0.9291	0.3573	1.0173	3.0747	1.1436	2.2639	-0.0036	0.0211	-0.1154	0.2943	0.1231	0.3142	0.2472	0.1246	-0.0523	0.4098	-0.0494	0.0452	0.5063	0.0581	0.5841	-36.4907	-12.4113	38.4061
31	1.1023	0.4521	-0.2077	5.8704	-0.3314	5.541	-0.2145	0.1653	-0.0194	-0.0629	-0.1177	0.8077	-0.2557	-0.9244	0.3122	0.438	0.0459	0.071	0.8025	-0.054	0.7486	-46.792	2.1517	38.0007
32	2.7562	-2.1301	0.531	5.7171	1.489	3.9094	0.2533	0.121	-0.3587	-0.2621	0.195	-0.1182	0.5875	0.0976	-0.5233	0.6125	-0.1889	-0.0147	0.5313	0.0516	0.6032	-37.8497	-27.8282	32.891
33	3.9008	-0.9049	-0.1622	8.2201	-0.8687	7.6376	0.2375	-0.1555	-0.6362	1.366	0.0281	0.8574	0.2508	-1.0173	-0.3949	0.4946	-0.1368	0.0068	0.666	0.0165	0.6914	-44.144	-16.5881	34.7452
34	9.0224	-1.7572	5.4605	9.0305	-0.2419	4.4748	-0.4687	-0.7645	-0.4	-0.4556	-0.229	0.5641	-1.1182	-0.5745	0.9492	0.4855	0.0223	-0.0222	0.871	-0.0514	0.4985	-42.9197	-1.5948	31.0132
35	5.4012	2.6328	-4.1816	4.5889	-5.2583	6.6196	0.0593	-1.1436	-0.532	1.1215	-1.2324	-1.5836	-0.9913	1.7704	0.6607	0.5786	-0.0055	0.0487	0.5056	-0.0393	0.4237	-29.4485	-13.6038	39.2556
36	0.8016	0.5033	1.5425	2.9484	-0.5953	4.1276	-0.175	-0.3136	1.4716	0.4433	0.8209	-1.3174	0.5105	0.4162	-0.323	0.4339	-0.074	0.0575	0.8228	0.2954	0.9214	-63.359	29.4708	29.87
37	3.1252	-0.172	-1.7512	5.21	0.8707	3.3647	-0.0945	0.2204	-0.3871	0.7145	-0.0838	0.5876	0.3177	-0.71	-0.0225	0.5004	0.0809	0.1302	0.8758	0.0636	0.7362	-62.1846	33.8574	28.3085
38	2.5452	0.0385	0.1117	3.0973	0.0985	1.7551	0.0563	-0.1411	-0.0765	0.2667	0.2165	0.1062	-0.1543	-0.5406	-0.1649	0.4484	0.1111	0.2352	0.8535	0.0949	0.6504	-53.1898	39.195	35.546
39	9.3597	-6.4921	3.0516	6.1653	-2.1722	3.2064	0.0843	-1.0135	-1.6973	0.4771	0.6373	1.1654	0.3917	-0.2265	-0.5979	0.6744	-0.0608	0.0896	0.7921	0.1371	0.8671	-52.3908	23.9479	43.1644
40	2.8182	1.7819	0.6127	4.2262	1.7355	0.4617	-0.1071	-0.1608	0.1242	0.8475	0.0307	0.9441	-0.4072	-0.3518	0.0215	0.5552	0.1261	0.2378	0.7373	-0.0286	0.5858	-53.4883	46.2581	42.3386
41	4.4561	1.2939	0.8403	0.7597	-0.8298	8.7235	-0.122	1.1282	-0.07	0.0271	-0.2467	0.3943	-0.2606	-0.2196	0.1205	0.4165	0.1481	0.1711	0.6966	-0.0279	0.5782	-52.6273	42.1867	26.1002
42	5.9257	-2.3061	1.6406	3.2499	-0.6505	3.4675	0.2043	0.1639	-0.8178	-0.4449	-0.0327	0.45	0.1726	-0.6034	-0.1737	0.4533	-0.0827	0.0036	0.7935	-0.1201	0.6227	-53.0969	28.6916	-11.4029
43	0.4121	-0.3869	-1.4606	4.2136	1.4058	4.1884	-0.0776	-0.0833	-0.3302	0.3503	-0.0093	0.4563	0.1612	-0.5558	0.0477	0.2484	-0.0045	-0.0079	0.7936	-0.0003	0.6735	-55.417	29.1079	5.5796
44	8.0962	-5.894	7.5302	4.9376	-5.4024	8.7503	0.0936	0.0723	-1.5913	-1.3942	0.6856	1.3835	1.829	-1.0947	-0.2838	0.4748	-0.1634	0.0161	0.8932	-0.0272	1.1484	-55.2635	12.9802	1.7513
45	3.4159	-2.4436	0.1944	2.0133	1.2623	1.3112	0.028	0.1934	0.1337	0.1154	0.0548	0.9503	-0.1815	-0.4829	-0.106	0.4264	0.0771	0.0228	0.9407	0.0313	0.9202	-66.3059	37.0966	4.4565
46	1.0289	0.6732	0.1339	6.9645	-4.2296	6.1081	0.0964	-0.3451	0.4427	-0.2358	-0.0892	0.7034	0.4225	0.5595	0.0522	0.3938	0.1618	-0.0374	0.6596	-0.1723	0.7342	-53.006	29.5351	3.8306
47	1.1989	1.174	-0.8129	2.3765	0.7061	7.7161	0.0831	-0.1294	0.4592	0.3076	0.0509	-0.1268	-0.7427	0.5242	0.1509	0.4435	-0.0315	-0.0485	0.5698	0.0638	0.8579	-1.6182	13.616	58.548
48	3.3081	1.2367	-0.2839	3.2548	-0.3452	1.4873	0.0633	-0.338	-0.0067	0.3594	-0.0256	-0.2919	0.043	0.2063	-0.0622	0.6287	0.0068	-0.2011	0.5952	0.03	0.4127	-9.0484	7.1103	54.9042
49	3.2593	-0.5034	0.1381	6.8186	-0.6172	1.339	0.2725	-0.4327	-0.1567	0.9042	-0.1824	-0.0202	0.0924	-0.2378	-0.0906	0.8331	-0.1059	-0.1005	0.6478	0.0569	0.4207	-17.0055	0.562	59.7874
50	7.6648	-0.9653	-1.4475	2.4748	1.5644	1.1114	0.6999	-1.1215	-1.5267	0.3919	-0.6626	0.5063	0.2165	0.4847	0.005	0.9362	-0.1055	-0.1611	0.7965	0.2403	0.5495	-13.5074	-2.0263	63.7431
51	4.6295	1.9194	1.6368	8.9196	-0.0876	9.2971	0.1063	-0.5342	0.2457	0.0758	-0.0131	1.0692	-0.6954	-0.3178	-0.0814	0.6023	-0.0348	-0.0191	0.6505	0.158	0.5704	-26.2644	10.0426	56.6744
52	7.7627	-3.8801	-1.6388	6.3427	3.1214	1.4701	0.327	0.8152	0.4821	0.1659	-0.2703	-0.169	-0.0636	0.0195	-0.0319	0.5037	0.0575	-0.0371	0.6152	0.0874	0.4215	-12.2247	6.6658	48.1348
53	3.5246	0.0427	0.3899	8.4127	3.472	5.3229	-0.1932	-0.0781	-0.3219	0.3269	0.7961	-0.3638	0.4171	0.4737	-0.5093	0.6909	0.059	0.0263	0.7702	0.1149	0.6903	-30.2667	49.442	51.3142
54	3.9484	1.5743	0.8054	3.021	-1.543	3.131	0.1447	-0.1165	-0.1442	0.37	0.1219	-0.4045	-0.3635	0.1313	-0.2508	0.5781	0.0015	0.1961	0.7518	-0.0857	0.442	-30.0226	53.9701	50.7971
55	3.3862	-0.5661	-1.3365	4.0799	0.6274	3.6723	-0.2327	0.1007	0.0549	0.6242	0.0728	0.1629	0.0702	-0.4106	0.0529	0.4199	0.0615	0.0241	0.5134	-0.0396	0.3765	-25.1585	56.4947	38.8504
56	2.9503	-0.094	-0.8425	2.339	0.7724	1.5453	0.2626	-0.3413	-0.4476	1.305	-0.4328	0.4338	0.1659	-0.0912	0.1345	0.7017	0.0135	0.0454	0.8212	0.0745	0.3649	-21.8846	43.5212	45.9303
57	5.6225	0.2222	-1.966	4.5217	0.0383	0.8846	-0.2815	-1.2187	-1.0194	1.0883	-0.391	-1.1464	-1.2359	0.6252	0.193	0.9762	-0.0495	-0.3153	0.6767	-0.0301	0.41	-13.8744	42.2506	49.6936
58	1.421	2.9675	-1.067	7.53	-3.8851	3.0568	0.3941	0.0297	0.4945	0.0978	0.4621	1.2997	-0.2701	-0.8105	-0.5821	0.7674	0.0777	0.1626	0.5833	-0.0726	0.8006	-20.3414	70.8834	35.2009
59	4.8534	-1.2785	-0.3486	2.0552	-0.4344	3.7042	-0.3878	-0.5176	0.2365	1.2225	-0.4855	0.2772	-0.1983	0.2392	0.6582	0.579	-0.0986	0.0144	0.5736	-0.0486	0.5865	-16.0856	59.8094	41.3444
60	3.2107	1.0255	1.6631	5.0953	0.1362	2.5449	-0.1648	-0.1531	-0.2868	0.125	0.1798	-0.2336	0.0171	0.1566	-0.0444	0.538	0.0602	0.1088	0.5466	-0.0023	0.3368	-21.3589	51.6188	37.6577
61	2.1754	-0.7668	-0.847	2.1409	0.2868	2.1617	-0.0678	0.2194	-0.1038	-0.3013	0.1152	0.328	0.3257	0.0139	-0.0347	0.8476	-0.0596	-0.2406	0.5244	0.0519	0.6076	5.6031	10.7853	95.9756
62	2.5324	0.3549	-0.8619	3.1493	1.6957	2.7319	0.0509	-0.0582	0.0857	-0.193	0.0433	0.4069	0.1259	-0.2302	-0.0611	0.7332	-0.0097	-0.2083	0.6263	0.0623	0.5995	2.8818	15.7469	106.1931
63	5.305	2.0833	0.9722	9.6969	0.4592	3.5229	0.505	-0.5072	-0.1644	1.7938	-0.6108	-0.1118	-0.0992	-0.7687	-0.3154	0.8024	0.1584	-0.1427	0.8814	0.0667	0.5355	-37.1404	51.1432	-42.3387
64	2.1755	1.2285	-0.8434	4.4084	-2.1325	2.1149	0.2763	-0.2928	0.3021	0.0554	-0.6713	-0.4187	-0.161	0.0579	0.2837	0.8827	0.1753	-0.1982	0.7734	-0.1791	0.7009	-37.022	54.5572	-44.2761
65	1.5064	0.6785	-0.6114	2.8242	0.3487	4.026	-0.0893	-0.22	0.3517	-0.2041	-0.1315	-0.2292	-0.4884	0.0096	0.1237	0.886	-0.0034	-0.2447	0.575	-0.0603	0.6127	-32.7947	51.6142	-55.4905
66	1.8467	0.8624	-1.4253	4.8929	-2.4218	1.3687	-0.1359	0.1369	0.4677	-0.4238	-0.0395	-0.6159	-0.6899	-0.256	0.2022	1.2452	0.0021	-0.1938	0.4456	-0.1705	1.1425	-31.755	68.5171	-61.5512
67	4.6622	3.694	0.1926	5.3483	2.8234	2.8937	0.286	-0.6512	-0.0849	-0.4906	-0.8437	-0.9909	0.545	1.6039	0.133	1.0096	-0.0674	-0.1973	0.8297	0.0023	0.7353	-20.8474	47.7838	-61.2625
68	4.7231	-2.9295	-1.4718	5.0533	3.1342	5.3083	-0.2484	0.3817	-0.1885	0.0146	-0.2752	0.1898	0.1776	-0.5276	0.4171	0.9736	-0.0986	-0.1973	0.4262	0.0387	0.6503	-37.5017	49.441	-59.226
69	3.7263	-0.1275	-3.1289	6.4294	5.2461	5.9232	-0.068	-0.475	0.1232	0.5777	0.4711	-1.0691	1.6077	-0.0816	0.1188	1.1189	0.2757	-0.1191	0.8681	0.0454	0.606	-71.5207	25.2508	-52.075
70	8.3189	-1.1843	-1.9831	4.1572	2.0634	4.5865	0.8932	0.8699	-0.0712	1.207	-1.8921	1.2031	0.1159	-2.2877	0.3802	0.9292	0.2172	0.2347	1.0863	-0.0066	0.757	-84.7686	45.6664	-34.5528
71	1.8428	0.8472	-0.2575	4.064	-0.1584	0.572	-0.0214	0.0375	0.1212	0.359	-0.0202	0.2159	0.0493	-0.025	-0.0051	0.7952	0.0635	-0.0994	0.5421	-0.0931	0.4843	-67.4654	37.239	-49.9322
72	6.2916	0.2211	2.2508	2.8961	-1.6618	2.3448	-0.7249	-0.8253	-0.7691	0.7354	0.9031	0.0877	0.7861	-0.8035	0.0383	1.6261	0.1077	0.1371	0.9405	0.0157	0.5868	-61.0998	25.8752	-38.9536
73	2.4425	-0.1831	-0.5821	6.7222	0.9945	2.8832	-0.13	-0.3686	0.5771	0.694	0.1035	0.504	-0.5375	-0.2883	0.1146	0.8883	0.1352	-0.0968	0.7056	-0.2452	0.7587	-64.1453	52.5055	-43.7109
74	3.1334	-2.9052	0.3929	5.4796	-3.0795	3.5608	-0.9443	-0.5796	-0.1234	-0.5488	0.0899	-0.3707	-0.4149	-0.2263	1.0857	0.8604	-0.0667	-0.1948	0.6233	0.0133	0.6238	-54.7752	32.8903	-43.129
75	5.5537	-2.7451	-0.8157	2.9693	0.0101	3.1053	-0.2062	0.9009	1.1373	0.3245	0.2887	-0.7229	0.0617	-0.1759	-0.1817	1.0766	0.1018	-0.2037	0.5447	-0.0939	0.6974	-68.9731	44.1992	-57.4122
76	4.7699	-1.9299	-1.0404	0.6165	0.0297	3.147	-0.3447	-0.0469	-0.8468	0.661	0.3329	0.2456	0.1581	-0.0803	0.1911	0.9928	-0.0903	0.0365	0.5177	-0.0763	0.9115	-35.6428	36.041	95.6947
77	3.6222	0.8847	2.311	2.981	0.9806	3.4865	-0.0356	0.0752	0.247	-0.3257	0.0876	-0.0415	0.5568	-0.048	0.045	0.9369	-0.1767	0.0205	0.536	0.1252	0.5903	-46.3079	35.872	91.3202
78	5.4341	2.5838	-0.1705	8.7848	-3.8797	7.4912	0.4217	0.2312	-0.3337	-0.4934	-0.6588	-0.5784	-0.1257	1.5834	0.0676	0.8262	-0.0606	0.1189	0.7293	0.0326	0.7837	-37.0788	24.1222	90.6217
79	1.9943	-1.1637	1.2012	3.0737	0.0511	3.3037	-0.3654	0.4196	0.6173	-0.4792	0.2015	-0.1615	-0.3078	0.1065	0.0992	1.2264	-0.2226	-0.0733	0.8323	0.2239	0.7299	-50.147	39.6523	78.2601
80	3.5687	-2.091	-0.5596	6.0103	4.404	4.6955	-0.0374	0.3489	-0.157	0.0528	0.0423	0.1891	0.4887	-0.2675	-0.0573	1.1918	-0.2314	-0.1781	0.695	0.2261	0.6467	-48.5351	30.0048	89.5297
81	3.4672	1.7206	-3.7015	6.5005	-2.8223	6.5551	-1.2351	-0.402	-0.2445	-0.4216	0.8726	-1.9188	0.6068	-0.6136	0.4158	1.3431	-0.1266	-0.1829	0.5617	-0.133	1.0616	-59.826	14.3867	72.2015
82	6.8193	-1.4893	2.2374	1.628	-0.0807	4.7776	1.1287	-0.1428	-0.8091	-0.006	0.1394	0.1892	-0.2167	0.4765	-0.4974	1.0964	-0.2704	0.1047	0.8688	0.1172	0.5675	-64.3606	18.9834	66.9203
83	4.4438	-0.4087	0.4796	4.7096	-2.0298	5.756	-0.6735	0.6882	0.5086	-0.8818	0.4849	-0.202	-1.1256	-0.929	0.2593	1.1716	-0.2468	-0.0885	1.0131	0.025	0.4793	-65.3839	24.7348	70.9524
84	5.7698	-0.9211	-0.8304	2.4818	-1.757	1.5116	-0.283	0.6468	-0.2105	-0.1317	0.004	0.2058	-0.4667	-0.168	0.2822	1.213	-0.2054	-0.3051	0.8876	0.0569	0.5993	-82.5394	18.3176	78.4458
85	5.2384	-2.1921	-2.8639	5.5842	-0.2578	3.8381	-0.6122	-0.0664	-0.6286	-1.2301	0.6107	0.5542	0.9693	0.173	0.0244	1.2494	-0.0997	0.0155	0.7772	-0.061	0.5611	-66.8052	4.9152	74.9764
86	9.51	-3.5379	2.3338	2.455	-1.211	0.8658	0.2138	2.0521	0.601	-0.6292	-0.7898	-0.5873	0.1563	0.6723	0.617	1.768	-0.1887	-0.2126	0.9279	0.1758	0.5971	-86.0497	18.7181	72.5453
87	3.2897	2.1035	3.6329	7.19	-0.4511	5.4498	-0.1694	-0.6496	0.3888	-2.1067	-0.5728	0.8076	-0.6905	0.196	0.4947	1.4045	-0.1538	-0.4716	0.7671	0.198	1.0421	-93.8725	31.663	72.5879
88	3.8718	-1.2227	0.0284	0.3338	-0.8469	2.0383	-0.4672	0.7543	0.0437	-1.1071	0.4773	0.0892	-0.0127	-0.0535	0.109	1.4317	-0.2824	-0.2418	0.8667	0.1129	0.5624	-81.1285	12.9355	72.8051
89	4.9215	1.8885	1.8562	3.9287	1.066	2.7597	-0.4719	-0.0874	0.2218	-0.3857	-0.7313	-0.6761	-0.6154	0.9944	0.8597	1.0406	0.0125	-0.0292	0.8138	0.0411	0.4841	-72.087	25.1939	81.1692
90	4.3617	4.0594	2.3127	7.3384	6.4922	5.2377	-0.8247	-0.4337	0.5897	-1.8373	0.3578	1.7029	-1.2633	-0.4538	0.4209	1.2317	0.1932	-0.0531	0.8633	0.1393	0.8929	18.1451	-34.1445	110.0089
91	3.4957	0.7676	-0.6807	2.8365	1.1311	2.4029	-0.1262	-0.2144	-0.1375	-0.5463	0.0373	0.1308	-0.6054	0.0189	-0.0184	1.4273	-0.0254	0.033	0.4959	0.0886	0.801	26.5894	-36.0997	106.5721
92	0.8208	-1.0092	-0.2231	1.7458	0.8405	2.1575	0.0215	0.077	0.0092	-0.8093	0.2349	-0.0873	-0.3926	-0.0973	-0.327	1.6578	-0.2377	0.1774	0.7035	0.0007	0.8114	30.6107	-30.1147	100.8437
93	3.0619	0.9091	-0.6122	0.5118	-0.5464	0.4198	-0.5419	0.4616	-0.129	-0.8251	0.2194	0.3357	-0.0754	-0.0758	0.2267	1.6747	-0.1391	0.0415	0.6042	-0.0056	0.7792	31.0089	-36.1814	97.3102
94	1.7688	1.2604	1.1797	0.7598	-2.2494	4.5949	-0.4186	0.2748	0.1061	-1.0504	0.2251	0.3966	-0.0883	-0.2296	-0.0474	1.7626	-0.0186	0.0531	0.706	-0.1038	0.7774	31.7596	-32.5972	98.739
95	3.885	4.2392	-2.965	6.7325	-2.6587	2.2754	0.1005	0.3381	0.9783	0.7562	0.5327	1.5912	-0.1097	-0.5292	-0.0611	1.5987	0.1691	0.0827	1.0278	-0.1365	0.804	-47.2588	0.4935	-35.511
96	3.3567	2.2705	3.2587	4.5578	2.2633	2.8267	-0.0386	-0.6097	0.0791	0.0805	-0.1874	0.0671	-0.0874	-0.3638	0.2217	1.2065	0.0495	0.1903	0.6356	-0.0033	0.5477	-48.7287	-12.0548	-43.7492
97	1.4572	0.399	0.6572	5.866	-2.0756	2.4311	-0.5449	-0.1918	0.3829	1.1417	0.4713	1.1204	0.1682	-0.3319	0.0133	1.3926	0.0923	0.1751	0.7028	-0.0975	0.7876	-57.0873	-3.2343	-42.6506
98	1.4119	0.2601	-0.0306	3.7905	0.765	-0.4287	0.0452	-0.3881	-0.0322	0.4017	-0.2391	0.3137	0.126	-0.0474	0.1629	1.5011	0.0202	0.2115	0.7398	0.0264	0.6432	-52.7886	-16.0539	-45.5884
99	4.949	-5.5075	5.5311	9.2121	-6.8582	6.0445	-0.7125	0.0241	1.1893	-0.5105	-0.2273	-1.0832	-0.137	-0.5593	1.3763	1.4725	0.1023	0.2269	1.05	0.1292	1.09	-13.2797	19.0899	-49.9996
100	3.8156	0.9565	2.6134	4.606	-1.8966	6.8651	-0.5759	0.3828	0.8339	-0.5635	-0.2278	-0.6485	-0.2454	0.8044	0.6193	1.1005	0.1535	0.2082	0.8135	-0.0027	0.8446	-10.4599	10.9668	-46.759
101	2.761	2.4355	-0.5192	4.5917	-2.5447	2.889	-0.253	0.1875	-0.1956	-0.0087	-0.0122	0.0668	0.1258	0.1211	0.295	1.3415	0.1751	0.0201	0.7686	-0.0505	0.6558	-21.8459	7.2276	-39.2246
102	4.3309	-1.2792	0.078	3.4436	-1.6719	-0.1409	-0.0086	-0.7544	0.2004	0.9057	-0.2204	-0.2139	-0.2198	0.2496	0.2036	1.3296	0.1139	-0.0104	0.9205	0.0405	0.7378	-23.3725	2.2091	-34.6343
103	5.3492	-0.1221	-1.3567	2.1418	-0.4669	8.026	-1.2091	1.5005	-1.8518	-1.4772	0.0835	-0.0274	0.8079	0.1333	0.549	1.9979	-0.1494	0.1425	0.4789	0.1075	0.8938	-17.8905	-1.7641	-48.043
104	3.5647	0.195	2.3516	2.728	0.5676	-0.2086	0.3619	0.2721	-0.1632	-0.1541	-0.1875	0.1126	0.2604	0.1446	-0.2154	1.743	-0.1008	0.4014	0.7724	0.0412	0.7985	-51.7264	-25.8962	82.3251
105	7.2261	-1.5308	3.7442	3.4182	-3.5933	3.6412	-0.6376	-0.0132	0.3101	-0.4874	0.4354	-0.7481	-0.3177	-0.4004	0.313	2.2289	-0.4295	0.4054	0.8269	-0.1097	0.9738	-46.2109	-18.5876	85.4504
106	4.6759	1.1219	1.6632	-0.2522	0.8323	0.0616	0.0335	0.5587	-0.2244	-0.0094	-0.1754	-0.1485	-0.1118	0.1787	0.0992	1.6774	-0.1117	0.4811	0.9242	-0.0177	0.8176	-39.5747	-23.3868	83.6741
107	6.0505	-3.2956	2.3599	4.4725	-1.7196	4.2373	1.0887	-1.527	0.7347	-0.1426	-0.3237	-1.3264	1.3188	-0.082	-0.2885	2.0928	-0.1867	0.3383	0.488	-0.0071	1.2448	-22.0484	-4.1121	92.5086
108	0.3903	-0.9216	0.656	1.8169	-1.3169	3.5433	-0.2884	0.8235	0.1711	-0.2503	-0.104	-0.3928	-0.254	-0.3005	0.346	1.5556	-0.1115	0.3681	1.0584	0.0509	1.1038	-21.1918	-6.9465	92.9783
109	3.7849	-1.6213	1.5021	1.7946	0.7252	2.516	-0.1857	1.0109	-0.2997	-1.14	-0.0041	-1.2697	0.5717	0.3106	0.004	1.9139	-0.0088	0.4576	0.8983	0.1793	1.0983	-27.1131	-12.1987	95.2667
110	8.5163	0.7364	6.4241	4.785	3.7025	6.9409	2.1345	-0.7193	0.2539	0.5574	1.0468	-0.6577	-0.2345	0.1457	0.0619	1.6799	0.0076	0.8438	1.1804	0.386	1.129	-14.371	-14.9438	94.8788
111	2.6588	0.3416	1.8211	0.0104	0.6775	4.4468	-0.275	-0.0291	0.0861	-0.2654	0.0627	-0.2926	-0.2712	0.2276	0.3696	1.6686	-0.0102	0.3886	0.7224	-0.0128	1.1602	-24.8769	-5.6438	110.064
112	2.8995	-0.0668	-0.87	2.4797	-1.5935	5.2723	0.3413	-0.0275	0.3715	-1.8515	-0.6097	0.0417	0.7991	0.993	0.2013	1.9456	-0.2105	0.3007	0.8591	0.2713	1.2934	-19.4031	0.262	107.9804
113	0.7968	0.0889	1.0845	5.0374	-4.9612	6.6365	-0.1928	-0.1562	-0.0774	1.312	-0.9074	0.3147	-0.873	0.0017	0.861	1.1564	0.0175	0.2702	1.001	-0.2554	1.3714	-12.4897	-16.6626	113.491
114	3.5028	0.945	-0.2315	2.2453	3.6852	7.6278	-0.4492	0.0468	0.1351	-1.7112	0.4416	-0.4508	-0.9277	0.2727	-0.0145	1.4052	-0.0891	0.367	0.8134	0.1549	0.7922	-26.7527	0.8143	110.5217
115	5.6936	0.2233	4.5986	1.7273	3.086	8.069	-1.6552	0.2739	0.0723	-2.7613	0.5195	-0.9062	-0.1663	-0.5274	0.4306	2.0809	-0.509	0.6153	0.6502	0.3045	1.0222	-26.8353	-17.8515	105.0548

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 7 through 18 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 19 through 63 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 64 through 127 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 128 through 175 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 176 through 229 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 230 through 284 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 285 through 301 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 8 through 27 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 28 through 41 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 42 through 80 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 81 through 107 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 108 through 127 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 128 through 149 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 150 through 163 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 164 through 207 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 208 through 229 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 230 through 300 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 7 through 37 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 38 through 128 )
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 129 through 242 )
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 243 through 300 )
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 8 through 37 )
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 38 through 163 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 164 through 288 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 289 through 300 )
26	X-RAY DIFFRACTION	26	chain 'E' and (resid 9 through 93 )
27	X-RAY DIFFRACTION	27	chain 'E' and (resid 94 through 300 )
28	X-RAY DIFFRACTION	28	chain 'F' and (resid 9 through 27 )
29	X-RAY DIFFRACTION	29	chain 'F' and (resid 28 through 63 )
30	X-RAY DIFFRACTION	30	chain 'F' and (resid 64 through 128 )
31	X-RAY DIFFRACTION	31	chain 'F' and (resid 129 through 163 )
32	X-RAY DIFFRACTION	32	chain 'F' and (resid 164 through 206 )
33	X-RAY DIFFRACTION	33	chain 'F' and (resid 207 through 228 )
34	X-RAY DIFFRACTION	34	chain 'F' and (resid 229 through 285 )
35	X-RAY DIFFRACTION	35	chain 'F' and (resid 286 through 300 )
36	X-RAY DIFFRACTION	36	chain 'G' and (resid 8 through 37 )
37	X-RAY DIFFRACTION	37	chain 'G' and (resid 38 through 80 )
38	X-RAY DIFFRACTION	38	chain 'G' and (resid 81 through 149 )
39	X-RAY DIFFRACTION	39	chain 'G' and (resid 150 through 163 )
40	X-RAY DIFFRACTION	40	chain 'G' and (resid 164 through 242 )
41	X-RAY DIFFRACTION	41	chain 'G' and (resid 243 through 299 )
42	X-RAY DIFFRACTION	42	chain 'H' and (resid 9 through 94 )
43	X-RAY DIFFRACTION	43	chain 'H' and (resid 95 through 149 )
44	X-RAY DIFFRACTION	44	chain 'H' and (resid 150 through 163 )
45	X-RAY DIFFRACTION	45	chain 'H' and (resid 164 through 229 )
46	X-RAY DIFFRACTION	46	chain 'H' and (resid 230 through 300 )
47	X-RAY DIFFRACTION	47	chain 'I' and (resid 8 through 37 )
48	X-RAY DIFFRACTION	48	chain 'I' and (resid 38 through 149 )
49	X-RAY DIFFRACTION	49	chain 'I' and (resid 150 through 205 )
50	X-RAY DIFFRACTION	50	chain 'I' and (resid 206 through 222 )
51	X-RAY DIFFRACTION	51	chain 'I' and (resid 223 through 235 )
52	X-RAY DIFFRACTION	52	chain 'I' and (resid 236 through 300 )
53	X-RAY DIFFRACTION	53	chain 'J' and (resid 9 through 37 )
54	X-RAY DIFFRACTION	54	chain 'J' and (resid 38 through 63 )
55	X-RAY DIFFRACTION	55	chain 'J' and (resid 64 through 127 )
56	X-RAY DIFFRACTION	56	chain 'J' and (resid 128 through 149 )
57	X-RAY DIFFRACTION	57	chain 'J' and (resid 150 through 163 )
58	X-RAY DIFFRACTION	58	chain 'J' and (resid 164 through 207 )
59	X-RAY DIFFRACTION	59	chain 'J' and (resid 208 through 228 )
60	X-RAY DIFFRACTION	60	chain 'J' and (resid 229 through 300 )
61	X-RAY DIFFRACTION	61	chain 'K' and (resid 9 through 149 )
62	X-RAY DIFFRACTION	62	chain 'K' and (resid 150 through 301 )
63	X-RAY DIFFRACTION	63	chain 'L' and (resid 9 through 37 )
64	X-RAY DIFFRACTION	64	chain 'L' and (resid 38 through 63 )
65	X-RAY DIFFRACTION	65	chain 'L' and (resid 64 through 175 )
66	X-RAY DIFFRACTION	66	chain 'L' and (resid 176 through 222 )
67	X-RAY DIFFRACTION	67	chain 'L' and (resid 223 through 235 )
68	X-RAY DIFFRACTION	68	chain 'L' and (resid 236 through 301 )
69	X-RAY DIFFRACTION	69	chain 'M' and (resid 9 through 27 )
70	X-RAY DIFFRACTION	70	chain 'M' and (resid 28 through 47 )
71	X-RAY DIFFRACTION	71	chain 'M' and (resid 48 through 149 )
72	X-RAY DIFFRACTION	72	chain 'M' and (resid 150 through 163 )
73	X-RAY DIFFRACTION	73	chain 'M' and (resid 164 through 228 )
74	X-RAY DIFFRACTION	74	chain 'M' and (resid 229 through 283 )
75	X-RAY DIFFRACTION	75	chain 'M' and (resid 284 through 300 )
76	X-RAY DIFFRACTION	76	chain 'N' and (resid 9 through 47 )
77	X-RAY DIFFRACTION	77	chain 'N' and (resid 48 through 127 )
78	X-RAY DIFFRACTION	78	chain 'N' and (resid 128 through 163 )
79	X-RAY DIFFRACTION	79	chain 'N' and (resid 164 through 229 )
80	X-RAY DIFFRACTION	80	chain 'N' and (resid 230 through 301 )
81	X-RAY DIFFRACTION	81	chain 'O' and (resid 11 through 27 )
82	X-RAY DIFFRACTION	82	chain 'O' and (resid 28 through 62 )
83	X-RAY DIFFRACTION	83	chain 'O' and (resid 63 through 94 )
84	X-RAY DIFFRACTION	84	chain 'O' and (resid 95 through 132 )
85	X-RAY DIFFRACTION	85	chain 'O' and (resid 133 through 163 )
86	X-RAY DIFFRACTION	86	chain 'O' and (resid 164 through 175 )
87	X-RAY DIFFRACTION	87	chain 'O' and (resid 176 through 203 )
88	X-RAY DIFFRACTION	88	chain 'O' and (resid 204 through 242 )
89	X-RAY DIFFRACTION	89	chain 'O' and (resid 243 through 301 )
90	X-RAY DIFFRACTION	90	chain 'P' and (resid 9 through 37 )
91	X-RAY DIFFRACTION	91	chain 'P' and (resid 38 through 112 )
92	X-RAY DIFFRACTION	92	chain 'P' and (resid 113 through 140 )
93	X-RAY DIFFRACTION	93	chain 'P' and (resid 141 through 208 )
94	X-RAY DIFFRACTION	94	chain 'P' and (resid 209 through 300 )
95	X-RAY DIFFRACTION	95	chain 'Q' and (resid 8 through 63 )
96	X-RAY DIFFRACTION	96	chain 'Q' and (resid 64 through 127 )
97	X-RAY DIFFRACTION	97	chain 'Q' and (resid 128 through 175 )
98	X-RAY DIFFRACTION	98	chain 'Q' and (resid 176 through 300 )
99	X-RAY DIFFRACTION	99	chain 'R' and (resid 11 through 27 )
100	X-RAY DIFFRACTION	100	chain 'R' and (resid 28 through 80 )
101	X-RAY DIFFRACTION	101	chain 'R' and (resid 81 through 175 )
102	X-RAY DIFFRACTION	102	chain 'R' and (resid 176 through 284 )
103	X-RAY DIFFRACTION	103	chain 'R' and (resid 285 through 301 )
104	X-RAY DIFFRACTION	104	chain 'S' and (resid 9 through 112 )
105	X-RAY DIFFRACTION	105	chain 'S' and (resid 113 through 149 )
106	X-RAY DIFFRACTION	106	chain 'S' and (resid 150 through 301 )
107	X-RAY DIFFRACTION	107	chain 'T' and (resid 17 through 27 )
108	X-RAY DIFFRACTION	108	chain 'T' and (resid 28 through 62 )
109	X-RAY DIFFRACTION	109	chain 'T' and (resid 63 through 81 )
110	X-RAY DIFFRACTION	110	chain 'T' and (resid 82 through 94 )
111	X-RAY DIFFRACTION	111	chain 'T' and (resid 95 through 149 )
112	X-RAY DIFFRACTION	112	chain 'T' and (resid 150 through 175 )
113	X-RAY DIFFRACTION	113	chain 'T' and (resid 176 through 222 )
114	X-RAY DIFFRACTION	114	chain 'T' and (resid 223 through 284 )
115	X-RAY DIFFRACTION	115	chain 'T' and (resid 285 through 300 )