温度: 293.5 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8 詳細: A co-crystal with lactose, grown from 12 mg/ml galectin-8N in 10 mM lactose, 10 mM Tris/HCl pH 8.0, 1 mM TCEP and 150 mM sodium chloride mixed with 25% (w/v) PEG 2000 MME) in a hanging drop, ...詳細: A co-crystal with lactose, grown from 12 mg/ml galectin-8N in 10 mM lactose, 10 mM Tris/HCl pH 8.0, 1 mM TCEP and 150 mM sodium chloride mixed with 25% (w/v) PEG 2000 MME) in a hanging drop, was used to soak the compound by transferring crystals in three steps to soaking drops. These were 2 uL drops with a soaking solution of 20% ethylene glycol, 25% PEG 2000 MME, 10 mM Tris pH 8, 1 mM TCEP, 50 mM NaCl, and 2 mM compound 10. The incubation times in the first two drops were about 10 min and in the third drop about 24 h. Then the crystals were transferred to a cryo solution with the same constituents and flash-frozen in liquid nitrogen.
タイプ: DECTRIS PILATUS3 X 6M / 検出器: PIXEL / 日付: 2021年9月18日 詳細: Oxford Danfysik/SESO Two stage demagnification using two K-B pairs of bimorph type mirrors
放射
モノクロメーター: ACCEL Fixed exit Double Crystal / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
構造決定の手法: 分子置換 / 解像度: 1.22→50.186 Å / Cor.coef. Fo:Fc: 0.975 / Cor.coef. Fo:Fc free: 0.964 / SU B: 2.242 / SU ML: 0.042 / 交差検証法: THROUGHOUT / ESU R: 0.047 / ESU R Free: 0.049 / 詳細: Hydrogens have been added in their riding positions
Rfactor
反射数
%反射
Selection details
Rfree
0.21391
4420
5.1 %
Random
Rwork
0.16897
82202
-
-
all
0.172
-
-
-
obs
0.17119
82202
99.875 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK BULK SOLVENT