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Open data
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Basic information
Entry | Database: PDB / ID: 8clj | |||||||||
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Title | TFIIIC TauB-DNA dimer | |||||||||
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![]() | TRANSCRIPTION / TFIIIC / tRNA gene / B-Box promoter / DNA recognition | |||||||||
Function / homology | ![]() tRNA transcription / 5S class rRNA transcription by RNA polymerase III / transcription factor TFIIIC complex / RNA polymerase III general transcription initiation factor activity / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Abortive And Retractive Initiation / transcription initiation at RNA polymerase III promoter / rRNA transcription / transcription by RNA polymerase III ...tRNA transcription / 5S class rRNA transcription by RNA polymerase III / transcription factor TFIIIC complex / RNA polymerase III general transcription initiation factor activity / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Abortive And Retractive Initiation / transcription initiation at RNA polymerase III promoter / rRNA transcription / transcription by RNA polymerase III / tRNA transcription by RNA polymerase III / histone acetyltransferase activity / histone acetyltransferase / enzyme activator activity / ribonucleoprotein complex / nucleolus / mitochondrion / DNA binding / nucleoplasm / membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
![]() | Seifert-Davila, W. / Girbig, M. / Hauptmann, L. / Hoffmann, T. / Eustermann, S. / Mueller, C.W. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into human TFIIIC promoter recognition. Authors: Wolfram Seifert-Davila / Mathias Girbig / Luis Hauptmann / Thomas Hoffmann / Sebastian Eustermann / Christoph W Müller / ![]() Abstract: Transcription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules τA and τB ...Transcription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules τA and τB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo-electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene. The τB module recognizes the B-box via DNA shape and sequence readout through the assembly of multiple winged-helix domains. TFIIIC220 forms an integral part of both τA and τB connecting the two subcomplexes via a ~550-amino acid residue flexible linker. Our data provide a structural mechanism by which high-affinity B-box recognition anchors TFIIIC to promoter DNA and permits scanning for low-affinity A-boxes and TFIIIB for Pol III activation. | |||||||||
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Structure visualization
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PDBx/mmCIF format | ![]() | 874 KB | Display | ![]() |
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PDB format | ![]() | 659.3 KB | Display | ![]() |
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-Validation report
Summary document | ![]() | 872.1 KB | Display | ![]() |
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Full document | ![]() | 886.1 KB | Display | |
Data in XML | ![]() | 89.4 KB | Display | |
Data in CIF | ![]() | 142.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 16714MC ![]() 8cliC ![]() 8clkC ![]() 8cllC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Deposited unit | ![]()
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Noncrystallographic symmetry (NCS) | NCS domain:
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