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Open data
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Basic information
Entry | Database: PDB / ID: 8ch1 | ||||||
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Title | PBP AccA from A. vitis S4 in complex with Agrocinopine A | ||||||
![]() | Agrocinopine utilization periplasmic binding protein AccA | ||||||
![]() | TRANSPORT PROTEIN / periplasmic binding protein / solute binding protein | ||||||
Function / homology | : / : / 2-O-phosphono-alpha-L-arabinopyranose / DI(HYDROXYETHYL)ETHER / 2-O-phosphono-beta-L-arabinopyranose![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Morera, S. / Vigouroux, A. | ||||||
Funding support | 1items
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![]() | ![]() Title: A highly conserved ligand-binding site for AccA transporters of antibiotic and quorum-sensing regulator in Agrobacterium leads to a different specificity. Authors: Morera, S. / Vigouroux, A. / Aumont-Nicaise, M. / Ahmar, M. / Meyer, T. / El Sahili, A. / Deicsics, G. / Gonzalez-Mula, A. / Li, S. / Dore, J. / Sirigu, S. / Legrand, P. / Penot, C. / Andre, ...Authors: Morera, S. / Vigouroux, A. / Aumont-Nicaise, M. / Ahmar, M. / Meyer, T. / El Sahili, A. / Deicsics, G. / Gonzalez-Mula, A. / Li, S. / Dore, J. / Sirigu, S. / Legrand, P. / Penot, C. / Andre, F. / Faure, D. / Soulere, L. / Queneau, Y. / Vial, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 297.3 KB | Display | ![]() |
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PDB format | ![]() | 242.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.8 MB | Display | ![]() |
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Full document | ![]() | 1.8 MB | Display | |
Data in XML | ![]() | 23.2 KB | Display | |
Data in CIF | ![]() | 35.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8c6rC ![]() 8c6uC ![]() 8c6wC ![]() 8c6yC ![]() 8c75C ![]() 8cawC ![]() 8cayC ![]() 8cb9C ![]() 8cdoC ![]() 8ch2C ![]() 8ch3C ![]() 8chcC ![]() 8ci6C ![]() 8cjuC ![]() 8ckdC ![]() 8ckeC ![]() 8ckoC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 56652.438 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() |
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-Sugars , 3 types, 4 molecules ![](data/chem/img/VDF.gif)
![](data/chem/img/LAO.gif)
![](data/chem/img/LAO.gif)
#2: Polysaccharide | Source method: isolated from a genetically manipulated source Details: phosphodiester of sucrose and L-arabinose / References: BIRD: PRD_002473 #3: Sugar | ChemComp-VDF / | #4: Sugar | ChemComp-LAO / | |
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-Non-polymers , 3 types, 455 molecules ![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | #6: Chemical | ChemComp-PEG / | #7: Water | ChemComp-HOH / | |
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-Details
Compound details | A member of the class of agrocinopines that consists of sucrose and L-arabinose units joined via a ...A member of the class of agrocinopines that consists of sucrose and L-arabinose units joined via a phosphodiester linkage between position 4F of sucrose and position 2 of arabinose. |
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Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.91 Å3/Da / Density % sol: 35.46 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: PEG 8000, HEPES |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Jun 29, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97933 Å / Relative weight: 1 |
Reflection | Resolution: 1.496→47.3 Å / Num. obs: 70531 / % possible obs: 99.5 % / Redundancy: 6.6 % / CC1/2: 0.99 / Rmerge(I) obs: 0.09 / Net I/σ(I): 15.9 |
Reflection shell | Resolution: 1.496→1.59 Å / Rmerge(I) obs: 1.303 / Num. unique obs: 10968 / CC1/2: 0.97 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 27.8 Å2
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Refine analyze | Luzzati coordinate error obs: 0.18 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.496→47.3 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.496→1.51 Å
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Refinement TLS params. | Origin x: 17.6356 Å / Origin y: -1.327 Å / Origin z: -19.1469 Å
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Refinement TLS group | Selection details: { A|* } |