PDB-8cfw: Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. ...

ID or keywords:

Entry

Database: PDB / ID: 8cfw

Title

Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with F2X-Entry library fragment H05

Components

Adenosylhomocysteinase

Keywords

HYDROLASE / Fragment screening / protein-ligand complex / drug discovery / SAM-DEPENDENT METHYLATION REACTIONS / SAHase / AHCY

Function / homology

Function and homology information

L-homocysteine biosynthetic process / adenosylhomocysteinase / adenosylhomocysteinase activity / S-adenosylmethionine cycle / one-carbon metabolic process / cytosol
Similarity search - Function

Biological species

Pseudomonas aeruginosa PAO1 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.61 Å

Authors

Malecki, P.H. / Gawel, M. / Stepniewska, M. / Brzezinski, K.

Funding support

Poland, 1items

Citation

Journal: To be published
Title: Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry H05
Authors: Malecki, P.H. / Gawel, M. / Stepniewska, M. / Brzezinski, K.

History

Deposition	Feb 3, 2023	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Feb 21, 2024	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8cfw.cif.gz	756.6 KB	Display	PDBx/mmCIF format
PDB format	pdb8cfw.ent.gz	625.3 KB	Display	PDB format
PDBx/mmJSON format	8cfw.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	8cfw_validation.pdf.gz	2.2 MB	Display	wwPDB validaton report
Full document	8cfw_full_validation.pdf.gz	2.2 MB	Display
Data in XML	8cfw_validation.xml.gz	81.2 KB	Display
Data in CIF	8cfw_validation.cif.gz	121.1 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/cf/8cfw ftp://data.pdbj.org/pub/pdb/validation_reports/cf/8cfw	HTTPS FTP

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Related structure data

Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#210 - Jun 2017 Adenine Riboswitch in Action similarity (1) #130 - Oct 2010 Riboswitches similarity (1) #156 - Dec 2012 ABO Blood Type Glycosyltransferases similarity (1)

Deposited unit

A: Adenosylhomocysteinase

B: Adenosylhomocysteinase

C: Adenosylhomocysteinase

D: Adenosylhomocysteinase

hetero molecules

213 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author
Evidence: gel filtration

Unit cell

Length a, b, c (Å)	73.830, 132.770, 98.480
Angle α, β, γ (deg.)	90.00, 100.94, 90.00
Int Tables number	4
Space group name H-M	P12₁1

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Protein , 1 types, 4 molecules ABCD

#1: Protein	Adenosylhomocysteinase / S-adenosyl-L-homocysteine hydrolase / AdoHcyase Mass: 51735.031 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa PAO1 (bacteria) / Strain: PAO1 / Gene: ahcY, sahH, PA0432 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 CodonPlus RILP / References: UniProt: Q9I685, adenosylhomocysteinase

#1: Protein

Adenosylhomocysteinase / S-adenosyl-L-homocysteine hydrolase / AdoHcyase

Mass: 51735.031 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Pseudomonas aeruginosa PAO1 (bacteria) / Strain: PAO1 / Gene: ahcY, sahH, PA0432 / Production host:

Escherichia coli (E. coli) / Strain (production host): BL21 CodonPlus RILP / References: UniProt: Q9I685, adenosylhomocysteinase

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Non-polymers , 7 types, 1636 molecules

#2: Chemical	ChemComp-NAD / NICOTINAMIDE-ADENINE-DINUCLEOTIDE Mass: 663.425 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₂₁H₂₇N₇O₁₄P₂ / Comment: NAD*YM
#3: Chemical	ChemComp-ADE / ADENINE Mass: 135.127 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₅H₅N₅
#4: Chemical	ChemComp-RE4 / 6-methyl-N~4~-[(pyridin-3-yl)methyl]pyrimidine-2,4-diamine Mass: 215.254 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₁₁H₁₃N₅ / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical	ChemComp-K / POTASSIUM ION Mass: 39.098 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: K
#6: Chemical	ChemComp-PO4 / PHOSPHATE ION Mass: 94.971 Da / Num. of mol.: 17 / Source method: obtained synthetically / Formula: PO₄
#7: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₃H₈O₃
#8: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 1601 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.29 Å³/Da / Density % sol: 46.29 %
Crystal grow	Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 50 mM KH2PO4, 20% (w/v) PEG8000, 20% (v/v) glycerol

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Data collection

Diffraction

Mean temperature: 100 K / Serial crystal experiment: N

Diffraction source

Source:

SYNCHROTRON / Site:

BESSY

/ Beamline: 14.2 / Wavelength: 0.9184 Å

Detector

Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Aug 27, 2021

Radiation

Monochromator: double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.9184 Å / Relative weight: 1

Reflection

Resolution: 1.61→46.12 Å / Num. obs: 234190 / % possible obs: 97.3 % / Redundancy: 4.66 % / CC_1/2: 0.998 / R_merge(I) obs: 0.087 / R_rim(I) all: 0.098 / Net I/σ(I): 10.87

Reflection shell

Resolution (Å)	R_merge(I) obs	Num. unique obs	CC_1/2	R_rim(I) all	Diffraction-ID
1.61-1.71	1.322	36900	0.472	1.5	1
1.71-1.82	0.829	35308	0.707	0.931	1
1.82-1.97	0.47	33033	0.888	0.527	1
1.97-2.16	0.251	30489	0.959	0.283	1
2.16-2.41	0.143	27736	0.984	0.163	1
2.41-2.78	0.089	24578	0.994	0.101	1
2.78-3.41	0.053	20876	0.997	0.059	1
3.41-4.8	0.032	16232	0.999	0.036	1
4.8-46.12	0.028	9038	0.999	0.031	1

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Processing

Software

Name	Version	Classification
PHENIX	1.19.2-4158	refinement
XDS		data scaling
XDS		data reduction
REFMAC		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 1.61→36.28 Å / SU ML: 0.19 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 19.78 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.1821	2458	1.05 %
R_work	0.158	-	-
obs	0.1582	234137	97.35 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 1.61→36.28 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	14228	0	361	1601	16190

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.007	15010
X-RAY DIFFRACTION	f_angle_d	0.857	20368
X-RAY DIFFRACTION	f_dihedral_angle_d	13.708	5570
X-RAY DIFFRACTION	f_chiral_restr	0.057	2267
X-RAY DIFFRACTION	f_plane_restr	0.007	2691

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.61-1.64	0.3129	130	0.3146	12281	X-RAY DIFFRACTION	93
1.64-1.67	0.3128	134	0.2884	12655	X-RAY DIFFRACTION	96
1.67-1.71	0.3398	135	0.2741	12708	X-RAY DIFFRACTION	97
1.71-1.75	0.2626	135	0.2522	12732	X-RAY DIFFRACTION	97
1.75-1.79	0.252	136	0.2274	12787	X-RAY DIFFRACTION	97
1.79-1.84	0.2079	136	0.2017	12806	X-RAY DIFFRACTION	97
1.84-1.9	0.2268	136	0.1851	12814	X-RAY DIFFRACTION	97
1.9-1.96	0.2022	136	0.1771	12783	X-RAY DIFFRACTION	97
1.96-2.03	0.2034	137	0.1646	12913	X-RAY DIFFRACTION	97
2.03-2.11	0.201	136	0.1562	12898	X-RAY DIFFRACTION	98
2.11-2.2	0.1888	137	0.1486	12896	X-RAY DIFFRACTION	98
2.2-2.32	0.2049	138	0.1508	12939	X-RAY DIFFRACTION	98
2.32-2.47	0.1971	138	0.1479	13001	X-RAY DIFFRACTION	98
2.47-2.66	0.1674	138	0.1528	13014	X-RAY DIFFRACTION	98
2.66-2.92	0.1867	138	0.1473	13015	X-RAY DIFFRACTION	98
2.92-3.35	0.1791	139	0.143	13098	X-RAY DIFFRACTION	99
3.35-4.21	0.1318	139	0.1249	13119	X-RAY DIFFRACTION	99
4.21-36.28	0.1471	140	0.148	13220	X-RAY DIFFRACTION	98

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.4543	-0.2335	-0.3079	0.941	0.8502	1.1456	0.0239	-0.0038	0.1476	-0.1412	0.0003	-0.0394	-0.197	0.0014	-0.0472	0.2841	0.0031	-0.0164	0.1437	0.007	0.2346	-4.1358	62.9618	-16.6784
2	1.6192	-0.0145	-0.5315	0.9693	0.3947	1.9064	0.0328	0.0095	0.1848	-0.0995	0.0043	0.0806	-0.1637	-0.1051	-0.0246	0.2323	0.0201	-0.0158	0.1288	-0.0173	0.2104	-12.7376	61.0909	-17.5653
3	2.0087	-1.8186	0.8793	3.7038	-2.6863	6.1072	-0.0721	-0.1134	0.0627	0.1703	0.204	0.4089	-0.4174	-0.7649	-0.1651	0.2396	0.0666	-0.0183	0.2972	-0.0413	0.3223	-25.3793	63.1663	-13.7233
4	5.7274	-0.7214	-1.7625	3.6531	0.7667	4.5612	-0.0133	0.083	0.0026	0.0359	-0.1543	0.4628	0.1448	-0.8413	0.1643	0.3004	-0.0173	-0.0571	0.3896	-0.0588	0.2573	-26.5763	58.303	-24.3109
5	0.9538	-0.836	0.9384	1.1487	-0.6753	1.8074	-0.0062	0.0141	-0.108	-0.2025	0.0096	0.2411	-0.0651	-0.2826	0.0059	0.2649	-0.0161	-0.0205	0.2331	-0.025	0.2596	-15.6867	44.8819	-25.5107
6	0.6172	0.23	0.3462	0.6573	0.3056	1.3686	0.0213	-0.1061	-0.0249	0.059	-0.0199	0.112	-0.0178	-0.2066	-0.0004	0.1435	0.0134	0.0147	0.1796	0.0124	0.1956	-15.6153	28.9187	-13.9128
7	1.9055	1.0668	-0.2984	3.3357	-1.3025	2.7521	0.044	-0.3309	0.0011	0.2959	-0.069	0.1396	-0.0591	-0.2686	-0.024	0.1612	0.0203	0.0243	0.2092	-0.0105	0.1656	-14.673	35.7347	-2.6045
8	1.4071	0.3295	-1.2306	2.9183	0.513	1.5555	0.0215	0.6483	0.0613	-0.5225	-0.1117	0.5378	-0.2387	-0.578	0.0413	0.3493	0.0626	-0.0727	0.3743	-0.0121	0.2357	-18.5318	58.5244	-34.8581
9	1.4153	0.1047	-0.2055	1.2998	0.4368	0.8034	0.0618	0.2102	0.1631	-0.2823	-0.0577	0.0337	-0.2828	0.0006	-0.0019	0.3154	-0.0017	-0.0016	0.1963	0.0451	0.1831	-1.3638	47.0626	-44.5779
10	6.1865	-5.763	3.4599	6.569	-1.9254	3.3378	-0.7068	0.1221	0.3035	-0.4696	0.3987	1.0096	-0.1771	0.1321	0.307	0.2293	0.0245	0.0315	0.143	0.0381	0.2461	-9.5775	49.5019	-19.0587
11	2.1946	0.5995	0.8441	2.492	0.9507	1.5139	-0.0981	-0.2106	0.2455	0.1801	0.0364	0.1283	-0.0826	-0.1485	0.062	0.2442	0.0651	0.0142	0.2522	-0.0003	0.1918	-1.1878	39.2942	11.9107
12	1.7294	-0.0235	1.5169	1.5389	-0.2938	3.147	0.0489	-0.3145	-0.1802	0.1833	0.0654	0.0546	0.2409	-0.1988	-0.1127	0.235	0.0456	0.0222	0.2478	0.0122	0.1622	7.7439	24.8019	13.3246
13	0.7482	0.0269	0.1431	1.3599	-0.167	0.6657	-0.0541	-0.1498	0.0568	0.1406	0.0385	0.0119	-0.1305	-0.0398	0.0149	0.2093	0.0348	-0.0113	0.2197	-0.0238	0.173	7.6381	37.9201	11.6017
14	1.6502	-1.0516	0.582	2.3045	-0.5079	1.8359	-0.1494	-0.1512	0.2407	0.1852	0.1153	-0.1254	-0.2479	0.0781	0.0347	0.2434	0.0128	-0.0542	0.217	-0.0541	0.1915	20.4089	39.7216	16.0611
15	1.0639	-0.6265	0.7761	0.6139	-0.3131	1.079	-0.0168	-0.0634	0.0429	0.0535	0.034	-0.0681	0.056	0.0914	-0.0054	0.1788	-0.0131	-0.0017	0.2062	-0.0055	0.1762	19.7005	30.8768	2.2655
16	0.7658	-0.4447	-0.1729	1.1402	0.389	0.5787	-0.0092	0.0508	0.1707	-0.0623	0.0456	-0.2096	-0.1407	0.1421	-0.0404	0.187	-0.0526	0.0126	0.1886	0.0084	0.2107	19.2348	43.8339	-16.7941
17	2.0781	0.3504	-0.7389	3.6654	-1.7987	2.4746	0.0395	0.0303	0.3975	0.1229	0.0347	-0.1716	-0.4275	0.065	-0.0538	0.1989	-0.0288	0.0064	0.1449	-0.02	0.2153	12.8052	53.3906	-10.2222
18	1.6857	0.423	1.1831	0.3336	0.2859	2.12	0.0439	-0.0892	-0.2365	0.1865	0.0858	-0.0716	0.1883	0.1247	-0.146	0.2258	0.0361	-0.0157	0.1735	-0.0083	0.1739	20.1479	20.8988	11.3283
19	1.925	-0.2121	0.0631	1.6058	-0.1966	1.4279	0.037	-0.196	-0.1738	0.0751	-0.0084	0.0166	0.1866	0.0547	-0.0226	0.1742	-0.0053	0.0002	0.169	0.0402	0.1659	10.9135	8.3268	-5.3232
20	3.8018	-1.996	-0.7221	5.7366	-4.8071	5.8737	-0.0942	-0.1753	-0.1918	-0.3098	0.0068	0.1401	-0.4318	0.33	0.0776	0.288	-0.0108	-0.0436	0.201	-0.0075	0.2242	9.4849	34.4954	0.6522
21	1.944	0.2985	0.562	1.6542	0.1959	2.304	0.1056	0.0972	-0.3832	0.0142	0.0134	-0.1916	0.4879	0.0817	-0.0904	0.2345	0.0415	0.019	0.2437	-0.0636	0.2839	31.8942	2.0997	-34.5205
22	2.6042	-0.4442	1.0817	0.3124	0.2683	1.1565	0.1371	-0.2549	-0.2539	0.1332	0.0027	-0.0302	0.2411	0.0392	-0.148	0.2279	-0.0194	0.0176	0.219	0.0369	0.2439	21.6133	8.1693	-21.3848
23	0.6019	0.0169	0.1699	0.6507	0.3215	1.7488	0.0647	0.0237	-0.1811	-0.0081	-0.0001	0.0444	0.2817	-0.0552	-0.0594	0.1785	-0.0033	-0.0146	0.1325	0.0108	0.2201	2.1139	4.4937	-24.3142
24	0.9461	-0.6542	-0.094	1.7532	1.2824	1.948	-0.0403	-0.0505	-0.0512	0.2237	0.1738	-0.2962	0.2465	0.3122	-0.1019	0.1555	-0.0077	-0.0071	0.2755	-0.0121	0.2667	34.9113	19.0909	-17.8378
25	1.9927	1.9952	1.9957	2.0043	2.0041	2.0039	0.011	-1.5337	-0.5989	-0.2706	-0.035	0.5776	0.2267	1.3625	0.0394	0.1319	0.0005	0.0113	0.3798	-0.071	0.3266	21.7975	9.4913	-31.3346
26	1.7423	-0.8885	-1.3943	1.6018	-0.8831	4.2965	0.1079	0.1772	-0.2378	-0.2833	-0.1275	-0.0145	0.398	0.0244	0.0278	0.2483	0.0289	-0.0611	0.2117	-0.0667	0.2572	-3.8182	3.419	-53.0579
27	3.0374	0.1235	0.5863	0.9754	-0.6051	2.5817	0.0622	-0.0331	-0.1781	-0.0997	-0.0098	0.1537	0.0866	-0.172	-0.0555	0.2157	0.0259	-0.0383	0.1823	-0.0533	0.2357	-17.419	13.2487	-50.6927
28	0.96	-0.2812	-0.0707	0.78	-0.1228	0.9135	0.0773	0.1777	-0.1429	-0.1603	-0.0365	0.0187	0.1685	0.0872	-0.0352	0.2625	0.0445	-0.034	0.2516	-0.0525	0.2116	-5.5689	11.6151	-56.3255
29	3.2045	-1.0554	0.4589	1.5943	0.1226	2.2642	0.1627	0.4015	-0.0877	-0.2851	-0.1746	-0.1217	0.0505	0.2769	0.0114	0.2972	0.0609	-0.0161	0.3098	-0.0276	0.1694	-7.6253	20.0515	-66.899
30	1.3198	-0.63	0.9993	0.4524	-0.3027	1.0086	0.012	0.1062	0.0474	-0.1248	-0.0548	0.0189	-0.0698	-0.0077	0.0586	0.2308	-0.0021	-0.0107	0.2284	-0.0037	0.1908	-8.1509	27.7261	-52.3376
31	1.3617	-0.6583	0.1655	1.3741	0.4887	0.7535	0.0092	0.087	0.1269	-0.0657	-0.0082	-0.09	-0.1481	0.1092	0.0127	0.2223	-0.0326	0.0171	0.1855	0.0155	0.1572	8.3363	36.8696	-38.0298
32	2.2679	0.1541	1.1106	2.0306	0.1806	1.0241	0.0309	0.1567	0.1641	-0.1731	-0.0459	-0.2049	-0.1892	0.2393	0.0273	0.2466	-0.0329	0.0652	0.289	0.0351	0.2104	14.7669	38.1796	-46.3024
33	1.753	-0.6169	-0.5495	1.5952	0.1512	0.9175	0.1023	0.3464	0.1033	-0.3713	-0.0757	-0.1583	-0.1577	0.1196	-0.0299	0.2777	-0.023	0.0572	0.3235	0.0217	0.1769	17.4243	28.6423	-52.7914
34	3.0851	0.626	-0.4148	1.9736	-0.2129	1.1989	0.061	0.3183	0.0073	-0.2847	-0.0418	-0.336	-0.1035	0.276	-0.0121	0.2369	0.0062	0.0614	0.2838	0.0064	0.1978	18.2507	23.7929	-50.8338
35	0.4522	0.0417	0.6996	0.429	-0.3128	2.4831	-0.0395	0.0562	0.0123	-0.0541	0.0105	0.16	-0.2307	-0.2148	0.0258	0.1957	0.0425	-0.0267	0.2298	-0.033	0.2078	-21.2754	25.4731	-54.081
36	1.6673	0.3475	0.213	1.4826	-0.2811	1.4999	0.0408	-0.0415	0.0218	-0.033	-0.0084	0.2194	-0.072	-0.2356	-0.0291	0.1505	0.0102	-0.0152	0.1946	-0.0054	0.1889	-22.605	27.8939	-31.7348
37	2.353	0.2809	1.2614	6.4487	-5.1961	5.1324	-0.3045	-0.0594	-0.0816	-0.4283	0.2805	0.8728	0.05	0.5195	-0.0066	0.2211	0.0358	-0.0291	0.3104	-0.0572	0.2281	-3.0838	19.0915	-47.8215

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 6 through 45 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 46 through 112 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 113 through 134 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 135 through 165 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 166 through 215 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 216 through 350 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 351 through 383 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 384 through 424 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 425 through 469 )
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 502 through 502 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 9 through 34 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 35 through 63 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 64 through 112 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 113 through 150 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 151 through 215 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 216 through 350 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 351 through 383 )
18	X-RAY DIFFRACTION	18	chain 'B' and (resid 384 through 424 )
19	X-RAY DIFFRACTION	19	chain 'B' and (resid 425 through 469 )
20	X-RAY DIFFRACTION	20	chain 'B' and (resid 503 through 503 )
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 10 through 165 )
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 166 through 215 )
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 216 through 383 )
24	X-RAY DIFFRACTION	24	chain 'C' and (resid 384 through 469 )
25	X-RAY DIFFRACTION	25	chain 'C' and (resid 502 through 502 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 8 through 34 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 35 through 63 )
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 64 through 112 )
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 113 through 150 )
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 151 through 215 )
31	X-RAY DIFFRACTION	31	chain 'D' and (resid 216 through 279 )
32	X-RAY DIFFRACTION	32	chain 'D' and (resid 280 through 305 )
33	X-RAY DIFFRACTION	33	chain 'D' and (resid 306 through 350 )
34	X-RAY DIFFRACTION	34	chain 'D' and (resid 351 through 383 )
35	X-RAY DIFFRACTION	35	chain 'D' and (resid 384 through 424 )
36	X-RAY DIFFRACTION	36	chain 'D' and (resid 425 through 469 )
37	X-RAY DIFFRACTION	37	chain 'D' and (resid 502 through 502 )

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