PDB-8cfp: Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. ...

ID or keywords:

Entry

Database: PDB / ID: 8cfp

Title

Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with F2X-Entry library fragment F09

Components

Adenosylhomocysteinase

Keywords

HYDROLASE / Fragment screening / protein-ligand complex / drug discovery / SAM-DEPENDENT METHYLATION REACTIONS / SAHase / AHCY

Function / homology

Function and homology information

adenosylhomocysteinase / adenosylhomocysteinase activity / S-adenosylmethionine cycle / one-carbon metabolic process / cytosol
Similarity search - Function

Biological species

Pseudomonas aeruginosa PAO1 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.61 Å

Authors

Malecki, P.H. / Gawel, M. / Stepniewska, M. / Brzezinski, K.

Funding support

Poland, 1items

Citation

Journal: To be published
Title: Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry F09
Authors: Malecki, P.H. / Gawel, M. / Stepniewska, M. / Brzezinski, K.

History

Deposition	Feb 3, 2023	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Feb 21, 2024	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8cfp.cif.gz	758.9 KB	Display	PDBx/mmCIF format
PDB format	pdb8cfp.ent.gz	628.3 KB	Display	PDB format
PDBx/mmJSON format	8cfp.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	8cfp_validation.pdf.gz	2.6 MB	Display	wwPDB validaton report
Full document	8cfp_full_validation.pdf.gz	2.7 MB	Display
Data in XML	8cfp_validation.xml.gz	82.4 KB	Display
Data in CIF	8cfp_validation.cif.gz	124.7 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/cf/8cfp ftp://data.pdbj.org/pub/pdb/validation_reports/cf/8cfp	HTTPS FTP

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Related structure data

Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#210 - Jun 2017 Adenine Riboswitch in Action similarity (1) #130 - Oct 2010 Riboswitches similarity (1) #156 - Dec 2012 ABO Blood Type Glycosyltransferases similarity (1)

Deposited unit

A: Adenosylhomocysteinase

B: Adenosylhomocysteinase

C: Adenosylhomocysteinase

D: Adenosylhomocysteinase

hetero molecules

213 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: gel filtration

Unit cell

Length a, b, c (Å)	73.830, 132.890, 98.710
Angle α, β, γ (deg.)	90.00, 101.06, 90.00
Int Tables number	4
Space group name H-M	P12₁1

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Protein , 1 types, 4 molecules ABCD

#1: Protein	Adenosylhomocysteinase / S-adenosyl-L-homocysteine hydrolase / AdoHcyase Mass: 51735.031 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa PAO1 (bacteria) / Strain: PAO1 / Gene: ahcY, sahH, PA0432 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 CodonPlus RILP / References: UniProt: Q9I685, adenosylhomocysteinase

#1: Protein

Adenosylhomocysteinase / S-adenosyl-L-homocysteine hydrolase / AdoHcyase

Mass: 51735.031 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Pseudomonas aeruginosa PAO1 (bacteria) / Strain: PAO1 / Gene: ahcY, sahH, PA0432 / Production host:

Escherichia coli (E. coli) / Strain (production host): BL21 CodonPlus RILP / References: UniProt: Q9I685, adenosylhomocysteinase

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Non-polymers , 8 types, 1725 molecules

#2: Chemical	ChemComp-NAD / NICOTINAMIDE-ADENINE-DINUCLEOTIDE Mass: 663.425 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₂₁H₂₇N₇O₁₄P₂ / Comment: NAD*YM
#3: Chemical	ChemComp-ADE / ADENINE Mass: 135.127 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₅H₅N₅
#4: Chemical	ChemComp-DMS / DIMETHYL SULFOXIDE Mass: 78.133 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₂H₆OS / Comment: DMSO, precipitant*YM
#5: Chemical	ChemComp-UI4 / 4-pyridin-2-ylphenol Mass: 171.195 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C₁₁H₉NO / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical	ChemComp-K / POTASSIUM ION Mass: 39.098 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: K
#7: Chemical	ChemComp-PO4 / PHOSPHATE ION Mass: 94.971 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: PO₄
#8: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C₃H₈O₃
#9: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 1686 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.3 Å³/Da / Density % sol: 46.44 %
Crystal grow	Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 50 mM KH2PO4, 20% (w/v) PEG8000, 20% (v/v) glycerol

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Data collection

Diffraction

Mean temperature: 100 K / Serial crystal experiment: N

Diffraction source

Source:

SYNCHROTRON / Site:

BESSY

/ Beamline: 14.2 / Wavelength: 0.9184 Å

Detector

Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Aug 27, 2021

Radiation

Monochromator: double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.9184 Å / Relative weight: 1

Reflection

Resolution: 1.61→48.44 Å / Num. obs: 235432 / % possible obs: 97.6 % / Redundancy: 4.64 % / CC_1/2: 0.998 / R_merge(I) obs: 0.099 / R_rim(I) all: 0.112 / Net I/σ(I): 10.3

Reflection shell

Resolution (Å)	R_merge(I) obs	Num. unique obs	CC_1/2	R_rim(I) all	Diffraction-ID
1.61-1.71	1.469	37151	0.411	1.668	1
1.71-1.82	0.976	35487	0.649	1.097	1
1.82-1.97	0.548	33262	0.858	0.615	1
1.97-2.16	0.293	30631	0.949	0.33	1
2.16-2.41	0.166	27895	0.98	0.188	1
2.41-2.78	0.105	24688	0.992	0.118	1
2.78-3.41	0.06	20979	0.997	0.067	1
3.41-4.81	0.032	16290	0.999	0.036	1
4.81-48.44	0.028	9049	0.999	0.031	1

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Processing

Software

Name	Version	Classification
PHENIX	1.19.2-4158	refinement
XDS		data scaling
XDS		data reduction
REFMAC		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 1.61→48.44 Å / SU ML: 0.2 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.54 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.1889	2471	1.05 %
R_work	0.1575	-	-
obs	0.1579	235354	97.61 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 1.61→48.44 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	14228	0	399	1686	16313

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.01	15071
X-RAY DIFFRACTION	f_angle_d	0.994	20447
X-RAY DIFFRACTION	f_dihedral_angle_d	13.898	5599
X-RAY DIFFRACTION	f_chiral_restr	0.066	2269
X-RAY DIFFRACTION	f_plane_restr	0.008	2703

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.61-1.64	0.3528	132	0.3262	12367	X-RAY DIFFRACTION	93
1.64-1.67	0.3595	135	0.304	12777	X-RAY DIFFRACTION	97
1.67-1.71	0.3328	136	0.2846	12731	X-RAY DIFFRACTION	97
1.71-1.75	0.2871	136	0.2681	12845	X-RAY DIFFRACTION	97
1.75-1.79	0.2808	136	0.2479	12832	X-RAY DIFFRACTION	97
1.79-1.84	0.274	137	0.2249	12851	X-RAY DIFFRACTION	97
1.84-1.9	0.2158	137	0.2057	12913	X-RAY DIFFRACTION	97
1.9-1.96	0.2527	136	0.1888	12849	X-RAY DIFFRACTION	98
1.96-2.03	0.2257	138	0.1714	12958	X-RAY DIFFRACTION	98
2.03-2.11	0.1982	136	0.1563	12994	X-RAY DIFFRACTION	98
2.11-2.2	0.1769	138	0.1438	12968	X-RAY DIFFRACTION	98
2.2-2.32	0.1719	138	0.143	12991	X-RAY DIFFRACTION	98
2.32-2.47	0.1913	139	0.1424	13074	X-RAY DIFFRACTION	99
2.47-2.66	0.1765	138	0.1444	13071	X-RAY DIFFRACTION	99
2.66-2.92	0.1935	140	0.1421	13115	X-RAY DIFFRACTION	99
2.92-3.35	0.1633	139	0.1375	13128	X-RAY DIFFRACTION	99
3.35-4.22	0.1344	140	0.1199	13189	X-RAY DIFFRACTION	99
4.22-48.44	0.1634	140	0.1439	13230	X-RAY DIFFRACTION	98

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.2392	-0.2904	-0.3051	0.9357	0.6989	0.7532	0.0302	-0.027	0.1646	-0.0969	-0.0192	-0.0462	-0.1821	-0.0199	-0.0305	0.2709	0.0108	-0.0342	0.1542	0.0023	0.2345	-4.1263	62.9336	-16.6477
2	1.4016	-0.0749	-0.2934	0.8496	0.4438	1.316	-0.0004	-0.0076	0.1925	-0.0679	0.0005	0.0447	-0.1671	-0.0545	0.0028	0.2219	0.0191	-0.0262	0.1368	-0.0191	0.2114	-12.7533	61.0556	-17.537
3	2.1022	-2.2562	1.3935	3.7274	-1.6673	4.9067	-0.1045	-0.2534	0.1204	0.1889	0.1106	0.4135	-0.4043	-0.6045	-0.0606	0.2036	0.0569	-0.0242	0.2662	-0.0691	0.3071	-25.4162	63.0495	-13.6671
4	4.4049	0.0576	-1.1562	2.4469	0.1893	3.9984	0.0079	0.015	0.1672	-0.0309	-0.0549	0.3584	-0.142	-0.5328	0.0528	0.247	0.0271	-0.0613	0.2559	-0.0414	0.256	-26.5764	58.2431	-24.2003
5	0.749	-0.589	0.6684	0.9212	-0.2776	1.4078	0.0356	0.0305	-0.0304	-0.1532	-0.0598	0.1664	-0.0336	-0.1381	0.0254	0.2112	-0.0084	-0.0191	0.1798	-0.0157	0.2129	-15.5546	44.7393	-25.3345
6	0.707	0.1102	0.3831	0.701	0.238	1.3945	0.02	-0.1072	-0.0373	0.0544	-0.0216	0.1248	0.0089	-0.1938	0.0022	0.1417	-0.0006	0.003	0.1687	0.0048	0.1805	-15.5428	28.9213	-13.9471
7	1.6257	0.9749	-0.1358	3.1268	-1.3192	2.4973	0.0616	-0.2999	-0.0177	0.3014	-0.0852	0.125	-0.0383	-0.2665	-0.0003	0.1516	0.007	0.019	0.2119	-0.0142	0.1523	-14.5575	35.6622	-2.6322
8	1.3479	0.1877	-1.079	2.171	0.5633	1.2763	0.0483	0.4576	0.1421	-0.4052	-0.1274	0.4186	-0.2369	-0.3777	0.0548	0.2968	0.0532	-0.0691	0.2462	-0.0063	0.2195	-18.5999	58.4475	-34.8863
9	1.4396	0.091	-0.2232	1.1879	0.2102	0.9245	0.0744	0.2074	0.1654	-0.2486	-0.0686	-0.0157	-0.2022	0.0099	-0.008	0.288	0.0108	-0.0079	0.1835	0.0402	0.1866	-1.3019	47.1147	-44.5674
10	4.8945	-4.4108	0.7656	5.917	1.3833	2.3363	-0.6584	0.015	0.52	-0.5305	0.2803	1.0254	-0.3031	0.0353	0.3723	0.2074	0.0056	0.0196	0.1703	0.0375	0.2442	-9.5657	49.5274	-19.0601
11	1.9499	0.5555	0.8533	2.0946	1.0378	1.6975	-0.0312	-0.238	0.2052	0.1642	-0.0564	0.0901	-0.0971	-0.1975	0.0825	0.2307	0.0469	0.013	0.2463	0.0015	0.1838	-1.1287	39.2959	11.8067
12	1.4449	-0.1721	1.4299	1.4314	-0.322	2.8179	0.0562	-0.262	-0.1625	0.1603	0.0736	0.0804	0.259	-0.1691	-0.1257	0.2399	0.0329	0.0146	0.2516	0.0094	0.1688	7.8413	24.8369	13.2514
13	0.7409	0.0112	0.0524	1.3257	-0.0329	0.6609	-0.0519	-0.1548	0.0522	0.1468	0.0382	0.0061	-0.0698	-0.0427	0.0093	0.2005	0.031	-0.0198	0.2123	-0.0183	0.1664	7.7039	37.9672	11.5309
14	1.3525	-0.9708	0.5123	2.1296	-0.3569	1.5407	-0.1525	-0.1357	0.2656	0.1683	0.109	-0.1024	-0.2414	0.0607	0.032	0.2531	0.0098	-0.0723	0.2361	-0.053	0.2179	20.4664	39.7842	15.9915
15	0.998	-0.669	0.6346	0.7785	-0.1987	0.9645	0.0088	-0.0798	0.0447	0.0362	0.0194	-0.0885	0.098	0.0684	-0.0157	0.1647	-0.0152	-0.0093	0.1796	-0.0025	0.1682	19.7043	30.8322	2.0974
16	0.8309	-0.431	-0.0341	0.9427	0.354	0.5527	-0.0016	0.024	0.1622	-0.0556	0.0477	-0.1832	-0.1173	0.1027	-0.0502	0.1698	-0.0458	0.0072	0.1599	0.0055	0.184	19.2598	43.8899	-16.8495
17	1.8552	0.2935	-0.7733	3.4783	-1.7809	2.4665	0.0601	0.03	0.3635	0.1254	0.0646	-0.1777	-0.3958	0.0367	-0.0882	0.2074	-0.0207	0.0081	0.1462	-0.0194	0.2319	12.813	53.4162	-10.3084
18	0.993	0.5118	0.4725	0.3231	0.4794	1.912	0.029	-0.0616	-0.2047	0.1609	0.0696	-0.1142	0.2333	0.219	-0.105	0.2117	0.0287	-0.0361	0.1568	-0.0024	0.1587	20.2047	20.9282	11.3017
19	1.6551	-0.0909	0.0157	1.5373	-0.0676	1.3335	0.0369	-0.1674	-0.154	0.0909	0.0012	0.009	0.1937	0.0596	-0.0308	0.1832	-0.01	-0.0176	0.1614	0.0337	0.1673	10.9599	8.3282	-5.3766
20	5.4518	3.2278	-5.3522	4.2637	-6.4142	9.7317	-0.1454	-0.1352	-0.1298	-0.2727	-0.0224	0.2269	-0.3141	0.2063	0.1695	0.2705	-0.0329	-0.0576	0.1889	0.0046	0.2173	9.5645	34.5268	0.6423
21	1.2638	0.5294	-0.0193	2.0722	-0.0594	2.1097	-0.0919	0.2894	-0.2148	-0.3792	0.2482	-0.2554	0.2143	0.14	-0.1623	0.2213	0.0271	0.0246	0.3035	-0.0707	0.2345	28.0787	7.4155	-45.8698
22	2.1674	0.1883	0.3524	1.8489	-0.5912	0.8336	-0.01	0.0931	-0.0111	0.0691	0.0254	-0.3317	0.0543	0.282	-0.017	0.1869	0.0182	0.0162	0.2951	-0.0614	0.2802	37.4299	11.9937	-32.3662
23	1.3614	0.1208	0.2549	1.2754	0.2185	1.6052	0.0743	0.1686	-0.4734	-0.077	0.0671	-0.2301	0.4103	0.1616	-0.1226	0.2764	0.0429	-0.02	0.2177	-0.082	0.32	30.1249	-1.1496	-36.4388
24	4.0858	-0.3698	-0.1011	4.6101	0.3086	2.8476	0.0866	-0.1006	-0.5213	0.1846	-0.0342	-0.2194	0.5275	0.0298	-0.0598	0.3633	0.0217	-0.0718	0.2637	0.0021	0.3478	33.6796	-4.0567	-23.0863
25	1.6823	-0.2495	0.5574	0.212	0.2747	0.8993	0.0831	-0.1554	-0.2049	0.1065	0.0279	-0.0429	0.1618	0.0392	-0.1175	0.2323	-0.0145	-0.0017	0.2066	0.0224	0.2505	21.5574	8.176	-21.4272
26	0.5363	0.0255	0.25	0.5919	0.3177	1.8637	0.0643	0.0053	-0.1518	0.0199	-0.0141	0.0422	0.2407	-0.0767	-0.0476	0.1814	-0.0075	-0.0207	0.1351	0.0093	0.2101	2.1645	5.6546	-22.0714
27	2.8711	1.0125	-0.4184	1.8159	-0.2337	1.7216	-0.0174	0.2022	-0.4272	-0.1308	0.0384	0.0506	0.3824	-0.1786	-0.0124	0.2526	-0.0071	-0.0418	0.1632	-0.034	0.2358	2.1788	-0.1952	-33.9125
28	2.3084	0.5449	-1.6094	2.5523	0.5374	1.6322	-0.0082	-0.3685	-0.3237	0.5738	0.0139	-0.4746	0.2865	0.5397	0.0572	0.271	0.0359	-0.0867	0.2734	-0.0261	0.2723	38.4311	7.8563	-19.8331
29	1.2169	-0.4193	0.1507	1.262	0.2677	1.6925	-0.0512	-0.0244	0.062	0.0119	0.1284	-0.3544	-0.1183	0.3211	-0.0811	0.1502	-0.037	-0.0003	0.2337	-0.022	0.2538	31.6625	29.3342	-16.1176
30	6.3484	-0.8269	0.5097	5.5467	-0.7314	6.3523	-0.2263	-1.0998	-0.0436	-0.4002	0.1021	0.5244	0.4417	0.9031	0.1382	0.1651	0.0034	-0.0148	0.3067	-0.0737	0.2965	21.8353	9.4865	-31.3458
31	0.8845	-0.2881	0.3144	0.8886	-0.1372	0.8843	0.1123	0.1935	-0.0984	-0.2075	-0.0809	0.0499	0.088	0.0337	-0.032	0.2387	0.0354	-0.051	0.2265	-0.0542	0.1735	-8.9281	14.1408	-58.3229
32	1.7239	-0.5986	0.9322	0.328	-0.187	1.1517	-0.0637	0.0694	0.1003	-0.0888	-0.0314	0.0149	-0.1639	-0.0189	0.1079	0.1989	-0.0049	-0.021	0.164	-0.0114	0.1662	-5.5116	28.6702	-48.4615
33	1.051	-0.4901	-0.1673	1.3502	0.3016	0.6499	0.0537	0.1818	0.1109	-0.1933	-0.0409	-0.1569	-0.1359	0.1166	-0.0104	0.2025	-0.0265	0.0219	0.2077	0.0231	0.1509	12.8046	34.2355	-44.8315
34	3.1004	0.5515	-0.329	2.0943	-0.3269	1.4871	0.065	0.4024	-0.0619	-0.315	-0.0764	-0.375	-0.0864	0.2448	0.0024	0.2363	0.0167	0.0479	0.2541	0.0047	0.1988	18.3525	23.8409	-50.8105
35	0.4176	0.1121	0.3854	0.3835	0.2091	1.5113	0.0179	0.0611	-0.0084	-0.0844	-0.0644	0.1328	-0.0987	-0.2416	0.0479	0.1424	0.0219	-0.0333	0.1951	-0.0199	0.171	-21.8638	26.7968	-42.4389
36	3.468	2.8212	0.8732	5.9217	-2.8584	3.7335	-0.2814	-0.2116	-0.0151	-0.2278	0.1446	0.6196	0.0646	0.5325	0.121	0.1632	0.0515	-0.0144	0.3036	-0.0558	0.1812	-2.9711	19.1466	-47.8876

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 6 through 45 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 46 through 112 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 113 through 134 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 135 through 165 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 166 through 215 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 216 through 350 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 351 through 383 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 384 through 424 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 425 through 469 )
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 502 through 502 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 9 through 34 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 35 through 63 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 64 through 112 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 113 through 150 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 151 through 215 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 216 through 350 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 351 through 383 )
18	X-RAY DIFFRACTION	18	chain 'B' and (resid 384 through 424 )
19	X-RAY DIFFRACTION	19	chain 'B' and (resid 425 through 469 )
20	X-RAY DIFFRACTION	20	chain 'B' and (resid 503 through 503 )
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 10 through 34 )
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 35 through 63 )
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 64 through 134 )
24	X-RAY DIFFRACTION	24	chain 'C' and (resid 135 through 165 )
25	X-RAY DIFFRACTION	25	chain 'C' and (resid 166 through 215 )
26	X-RAY DIFFRACTION	26	chain 'C' and (resid 216 through 350 )
27	X-RAY DIFFRACTION	27	chain 'C' and (resid 351 through 383 )
28	X-RAY DIFFRACTION	28	chain 'C' and (resid 384 through 424 )
29	X-RAY DIFFRACTION	29	chain 'C' and (resid 425 through 469 )
30	X-RAY DIFFRACTION	30	chain 'C' and (resid 502 through 502 )
31	X-RAY DIFFRACTION	31	chain 'D' and (resid 8 through 165 )
32	X-RAY DIFFRACTION	32	chain 'D' and (resid 166 through 215 )
33	X-RAY DIFFRACTION	33	chain 'D' and (resid 216 through 350 )
34	X-RAY DIFFRACTION	34	chain 'D' and (resid 351 through 383 )
35	X-RAY DIFFRACTION	35	chain 'D' and (resid 384 through 469 )
36	X-RAY DIFFRACTION	36	chain 'D' and (resid 502 through 502 )

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