PDB-8cfm: Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. ...

ID or keywords:

Entry

Database: PDB / ID: 8cfm

Title

Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with F2X-Entry library fragment E08

Components

Adenosylhomocysteinase

Keywords

HYDROLASE / Fragment screening / protein-ligand complex / drug discovery / SAM-DEPENDENT METHYLATION REACTIONS / SAHase / AHCY

Function / homology

Function and homology information

L-homocysteine biosynthetic process / adenosylhomocysteinase / adenosylhomocysteinase activity / S-adenosylmethionine cycle / one-carbon metabolic process / cytosol
Similarity search - Function

Biological species

Pseudomonas aeruginosa PAO1 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.84 Å

Authors

Malecki, P.H. / Gawel, M. / Stepniewska, M. / Brzezinski, K.

Funding support

Poland, 1items

Citation

Journal: To be published
Title: Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry E08
Authors: Malecki, P.H. / Gawel, M. / Stepniewska, M. / Brzezinski, K.

History

Deposition	Feb 3, 2023	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Feb 21, 2024	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8cfm.cif.gz	757.5 KB	Display	PDBx/mmCIF format
PDB format	pdb8cfm.ent.gz	627.3 KB	Display	PDB format
PDBx/mmJSON format	8cfm.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	8cfm_validation.pdf.gz	2.1 MB	Display	wwPDB validaton report
Full document	8cfm_full_validation.pdf.gz	2.1 MB	Display
Data in XML	8cfm_validation.xml.gz	79.8 KB	Display
Data in CIF	8cfm_validation.cif.gz	117.6 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/cf/8cfm ftp://data.pdbj.org/pub/pdb/validation_reports/cf/8cfm	HTTPS FTP

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Related structure data

Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#210 - Jun 2017 Adenine Riboswitch in Action similarity (1) #130 - Oct 2010 Riboswitches similarity (1) #156 - Dec 2012 ABO Blood Type Glycosyltransferases similarity (1)

Deposited unit

A: Adenosylhomocysteinase

B: Adenosylhomocysteinase

C: Adenosylhomocysteinase

D: Adenosylhomocysteinase

hetero molecules

213 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author
Evidence: gel filtration

Unit cell

Length a, b, c (Å)	74.050, 133.220, 98.280
Angle α, β, γ (deg.)	90.00, 100.94, 90.00
Int Tables number	4
Space group name H-M	P12₁1

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Protein , 1 types, 4 molecules ABCD

#1: Protein	Adenosylhomocysteinase / S-adenosyl-L-homocysteine hydrolase / AdoHcyase Mass: 51735.031 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa PAO1 (bacteria) / Strain: PAO1 / Gene: ahcY, sahH, PA0432 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 CodonPlus RILP / References: UniProt: Q9I685, adenosylhomocysteinase

#1: Protein

Adenosylhomocysteinase / S-adenosyl-L-homocysteine hydrolase / AdoHcyase

Mass: 51735.031 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Pseudomonas aeruginosa PAO1 (bacteria) / Strain: PAO1 / Gene: ahcY, sahH, PA0432 / Production host:

Escherichia coli (E. coli) / Strain (production host): BL21 CodonPlus RILP / References: UniProt: Q9I685, adenosylhomocysteinase

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Non-polymers , 8 types, 1502 molecules

#2: Chemical	ChemComp-NAD / NICOTINAMIDE-ADENINE-DINUCLEOTIDE Mass: 663.425 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₂₁H₂₇N₇O₁₄P₂ / Comment: NAD*YM
#3: Chemical	ChemComp-ADE / ADENINE Mass: 135.127 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₅H₅N₅
#4: Chemical	ChemComp-UHL / 5-(2-methoxyethyl)-1,3,4-oxadiazol-2-amine Mass: 143.144 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₅H₉N₃O₂ / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical	ChemComp-K / POTASSIUM ION Mass: 39.098 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: K
#6: Chemical	ChemComp-PO4 / PHOSPHATE ION Mass: 94.971 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: PO₄
#7: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₃H₈O₃
#8: Chemical	ChemComp-DMS / DIMETHYL SULFOXIDE Mass: 78.133 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₂H₆OS / Comment: DMSO, precipitant*YM
#9: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 1467 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.3 Å³/Da / Density % sol: 46.52 %
Crystal grow	Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 50 mM KH2PO4, 20% (w/v) PEG8000, 20% (v/v) glycerol

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Data collection

Diffraction

Mean temperature: 100 K / Serial crystal experiment: N

Diffraction source

Source:

SYNCHROTRON / Site:

BESSY

/ Beamline: 14.2 / Wavelength: 0.9184 Å

Detector

Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Aug 27, 2021

Radiation

Monochromator: double crystal / Protocol: SINGLE WAVELENGTH / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.9184 Å / Relative weight: 1

Reflection

Resolution: 1.84→36.37 Å / Num. obs: 158778 / % possible obs: 97.9 % / Redundancy: 4.66 % / CC_1/2: 0.997 / R_merge(I) obs: 0.115 / R_rim(I) all: 0.13 / Net I/σ(I): 9.69

Reflection shell

Resolution (Å)	R_merge(I) obs	Num. unique obs	CC_1/2	R_rim(I) all	Diffraction-ID
1.84-1.95	1.595	25098	0.405	1.793	1
1.95-2.08	0.923	23944	0.64	1.041	1
2.08-2.25	0.526	22388	0.825	0.6	1
2.25-2.46	0.307	20692	0.93	0.349	1
2.46-2.75	0.188	18773	0.975	0.213	1
2.75-3.18	0.106	16665	0.991	0.119	1
3.18-3.88	0.054	14088	0.997	0.062	1
3.88-5.46	0.034	10914	0.998	0.039	1
5.46-36.37	0.03	6216	0.999	0.033	1

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Processing

Software

Name	Version	Classification
PHENIX	1.19.2-4158	refinement
XDS		data scaling
XDS		data reduction
REFMAC		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 1.84→36.37 Å / SU ML: 0.25 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 22.06 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2012	2099	1.32 %
R_work	0.1666	-	-
obs	0.1671	158726	97.95 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 1.84→36.37 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	14228	0	348	1467	16043

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.004	15002
X-RAY DIFFRACTION	f_angle_d	0.627	20355
X-RAY DIFFRACTION	f_dihedral_angle_d	13.108	5574
X-RAY DIFFRACTION	f_chiral_restr	0.046	2267
X-RAY DIFFRACTION	f_plane_restr	0.004	2692

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.84-1.88	0.3257	135	0.3304	10038	X-RAY DIFFRACTION	94
1.88-1.93	0.32	136	0.3009	10338	X-RAY DIFFRACTION	97
1.93-1.98	0.3131	139	0.2697	10340	X-RAY DIFFRACTION	98
1.98-2.04	0.2751	139	0.2484	10360	X-RAY DIFFRACTION	98
2.04-2.1	0.291	140	0.2298	10425	X-RAY DIFFRACTION	98
2.1-2.18	0.249	140	0.2029	10455	X-RAY DIFFRACTION	98
2.18-2.27	0.2318	139	0.195	10395	X-RAY DIFFRACTION	98
2.27-2.37	0.1976	141	0.1747	10475	X-RAY DIFFRACTION	98
2.37-2.5	0.2396	140	0.1709	10451	X-RAY DIFFRACTION	98
2.5-2.65	0.2217	140	0.1683	10492	X-RAY DIFFRACTION	98
2.65-2.86	0.209	142	0.163	10542	X-RAY DIFFRACTION	99
2.86-3.14	0.2101	141	0.1556	10507	X-RAY DIFFRACTION	99
3.14-3.6	0.1642	142	0.1398	10604	X-RAY DIFFRACTION	99
3.6-4.53	0.1523	142	0.1223	10573	X-RAY DIFFRACTION	99
4.53-36.37	0.1681	143	0.1489	10632	X-RAY DIFFRACTION	98

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.5498	-0.2142	-0.5723	1.0315	1.1391	1.415	0.0094	0.0119	0.1925	-0.1139	0.0209	-0.1057	-0.177	-0.0734	-0.0853	0.3598	-0.0008	-0.0196	0.1906	0.0139	0.3211	-4.1519	63.0942	-16.6615
2	1.8831	-0.0265	-0.2773	1.2902	0.7192	2.3494	0.0371	-0.0639	0.1913	0.0156	-0.026	0.0033	-0.1716	-0.1572	-0.001	0.2915	0.0111	-0.0141	0.1968	-0.025	0.2663	-12.7664	61.2342	-17.5239
3	3.217	-1.7809	0.9308	3.345	-1.4708	7.5775	-0.0969	-0.22	0.12	0.3373	0.004	0.4649	-0.4734	-0.8976	0.0481	0.2973	0.0317	-0.0018	0.3655	-0.0965	0.3743	-25.4154	63.327	-13.6742
4	5.6903	-0.6153	-1.881	3.2635	1.0467	4.5317	-0.0134	0.0746	0.0893	0.192	-0.0751	0.4349	0.1684	-0.7842	0.0961	0.3311	-0.0183	-0.0637	0.4054	-0.0456	0.2865	-26.5879	58.3836	-24.2684
5	0.9315	-1.0008	0.8839	1.0026	-0.772	1.5725	0.0096	0.0486	-0.1147	-0.1491	0.015	0.2016	-0.0516	-0.2318	-0.0243	0.3041	-0.0277	-0.0126	0.2459	-0.0148	0.2661	-15.6365	44.858	-25.2874
6	0.8249	0.1242	0.2207	0.7343	0.5265	1.6756	0.0328	-0.1392	-0.031	0.0349	-0.0451	0.1556	-0.018	-0.2449	0.0161	0.1911	0.0137	0.0138	0.2189	0.0087	0.2587	-15.6315	29.0409	-13.7764
7	2.0384	1.2196	-0.4393	3.9347	-1.735	3.4959	0.0279	-0.3675	-0.0457	0.2828	-0.1591	0.1022	-0.1084	-0.2273	0.0859	0.1849	0.0123	0.0135	0.2618	-0.0192	0.2305	-14.6274	35.8328	-2.49
8	1.3204	0.5343	-1.2769	3.2377	0.7142	1.8719	0.0118	0.5408	0.1399	-0.5593	-0.0845	0.4727	-0.1789	-0.7012	0.0149	0.3939	0.0503	-0.0687	0.4122	0.0047	0.2536	-18.6099	58.6585	-34.7945
9	1.7739	0.1483	-0.1779	1.4908	0.1602	0.8915	0.084	0.2621	0.238	-0.2897	-0.0963	-0.003	-0.219	0.0473	-0.0044	0.3946	0.0213	-0.0044	0.2425	0.0511	0.2441	-1.3525	47.1439	-44.4956
10	2.1703	-0.3062	1.6602	3.3702	-2.773	3.2131	-1.0261	0.398	0.3412	-0.8566	0.8982	1.6462	-0.3061	0.3159	0.1545	0.2445	-0.0303	-0.0258	0.2373	0.0791	0.3141	-9.675	49.6526	-18.9704
11	2.5484	0.3714	0.7193	3.2877	1.1184	1.4584	-0.1249	-0.1986	0.3263	0.1576	0.0305	0.1892	-0.1631	-0.1826	0.0816	0.2607	0.0861	0.0195	0.3078	0.004	0.2207	-1.1299	39.4129	12.0249
12	1.9105	-0.0507	1.5947	1.1097	-0.2265	3.3127	0.0446	-0.3126	-0.2146	0.2633	0.0979	0.0602	0.253	-0.1888	-0.1309	0.2894	0.0609	0.0264	0.2919	0.0032	0.2072	7.8367	24.9256	13.423
13	0.9626	-0.1683	0.1721	1.5193	-0.0062	0.7293	-0.1005	-0.1799	0.0824	0.1091	0.0974	0.0349	-0.087	-0.114	0.0097	0.2477	0.0485	-0.0181	0.2623	-0.03	0.2037	7.7182	38.0581	11.6979
14	2.0256	-1.2747	0.6147	2.3328	-0.3259	2.3308	-0.1747	-0.2154	0.33	0.2329	0.1644	-0.2128	-0.2721	0.1055	0.0144	0.2892	0.0323	-0.0648	0.2727	-0.0707	0.2593	20.5172	39.8876	16.1177
15	1.1055	-0.8474	0.6715	0.9496	-0.1321	0.9927	-0.0126	-0.055	0.0419	0.0427	0.0445	-0.1063	0.0767	0.1639	-0.0079	0.1696	-0.0171	0.0109	0.2353	0.0005	0.198	19.7467	31.0009	2.315
16	1.023	-0.5177	-0.1646	1.2857	0.3371	0.5727	-0.0021	0.0529	0.2363	-0.1014	0.04	-0.2766	-0.2059	0.1856	-0.0376	0.2259	-0.0705	0.0213	0.2356	0.0062	0.2699	19.2258	43.9454	-16.7427
17	2.8093	0.6246	-0.6598	3.9687	-2.0535	3.3303	-0.0035	0.0425	0.5094	0.121	0.0793	-0.2007	-0.5807	-0.0062	-0.043	0.2527	-0.0151	0.0138	0.1996	-0.0304	0.2975	12.773	53.5001	-10.2146
18	1.2348	0.6211	0.5056	0.3314	0.4232	2.0638	-0.034	-0.1272	-0.2415	0.236	0.1174	-0.1206	0.1775	0.215	-0.1242	0.2735	0.0565	-0.0124	0.2042	-0.009	0.221	20.1798	20.9851	11.3713
19	2.1443	-0.0391	0.3311	2.0898	-0.4027	1.9175	0.0541	-0.2142	-0.2006	0.0659	0.0205	-0.0032	0.2311	0.072	-0.0683	0.2059	-0.0006	0.002	0.2138	0.034	0.2201	10.8759	8.4285	-5.1907
20	4.0314	1.4104	-1.5565	6.823	1.4667	4.0071	-0.4814	-0.0677	-0.1096	-0.3686	-0.1411	0.1568	-0.2202	0.0901	0.5973	0.3711	-0.059	-0.0501	0.2444	0.0276	0.298	9.4809	34.6201	0.7231
21	2.4189	0.3017	0.8987	1.8944	0.4948	4.4207	0.2827	0.0575	-0.4378	0.0577	0.0276	-0.2567	0.985	-0.0912	-0.2551	0.4168	-0.0025	-0.0229	0.3243	-0.085	0.3787	31.9652	2.1546	-34.3518
22	2.8207	-0.0947	1.0412	0.3678	0.5712	1.3613	0.2803	-0.2949	-0.4049	0.2233	-0.0362	-0.0157	0.3405	0.0541	-0.2559	0.3216	-0.0361	-0.0057	0.2696	0.0275	0.2835	21.5818	8.252	-21.2234
23	0.6942	0.1038	0.1554	0.886	0.3152	2.3122	0.0841	0.0391	-0.2453	-0.0243	-0.001	0.0303	0.3917	-0.0413	-0.0794	0.2278	0.0194	-0.0281	0.1555	0.0034	0.2734	2.0307	4.583	-24.1476
24	0.9693	-0.5661	0.038	2.1658	1.5596	2.4073	0.0144	-0.0194	-0.0357	0.2795	0.2007	-0.4462	0.2906	0.331	-0.1709	0.1899	-0.0109	-0.0058	0.3317	-0.042	0.3332	34.9184	19.1786	-17.7184
25	2	-8.5049	-2.0584	9.7792	-6.2106	2	0.5258	-1.7925	-0.8345	0.0317	-0.4052	0.3727	-0.1926	1.6899	-0.1055	0.2011	-0.087	-0.0159	0.6554	-0.2099	0.4443	21.7446	9.3928	-31.2894
26	1.5662	-0.4978	-1.3386	1.5338	-0.7804	4.2675	0.0815	0.2398	-0.3002	-0.3213	-0.1683	-0.0113	0.3517	-0.0135	0.0835	0.3192	0.0654	-0.08	0.2504	-0.0943	0.3054	-3.9335	3.4793	-52.8874
27	2.1768	-0.2182	0.5036	1.1081	-0.4714	2.6184	0.0736	0.0775	-0.2125	-0.1456	-0.0148	0.2499	0.1509	-0.1366	-0.0676	0.2737	0.0287	-0.0568	0.2185	-0.0585	0.306	-17.4682	13.356	-50.5618
28	0.7665	-0.2187	-0.1431	1.1298	-0.1003	0.9721	0.0546	0.1808	-0.1505	-0.2023	-0.0506	-0.0236	0.2065	0.1384	-0.0079	0.3384	0.0744	-0.0555	0.3122	-0.0792	0.2585	-5.659	11.687	-56.1852
29	2.8587	-0.958	0.3866	1.9239	0.1945	2.8101	0.2618	0.4923	-0.0535	-0.483	-0.2337	-0.2119	0.0869	0.2854	-0.0051	0.3725	0.0857	0.0012	0.3403	-0.0296	0.2202	-7.7183	20.1256	-66.7806
30	1.4379	-0.7885	0.9085	0.5223	-0.3146	1.0391	0.0333	0.174	0.0858	-0.1475	-0.0787	-0.001	-0.0851	0.0221	0.0405	0.2576	0.0082	-0.0167	0.2354	-0.0094	0.2205	-8.1886	27.8193	-52.1967
31	1.6814	-0.6864	0.1448	1.4968	0.405	0.7832	0.0127	0.1276	0.1267	-0.1255	0.0042	-0.0755	-0.218	0.121	-0.0077	0.2974	-0.0242	0.0215	0.2173	0.0151	0.1881	8.2895	37.0216	-37.9962
32	1.7015	-0.4101	1.2155	1.8407	0.3043	1.0733	0.1628	0.3495	0.2527	-0.2391	-0.1519	-0.1925	-0.1956	0.4481	-0.0186	0.315	-0.0253	0.0956	0.3773	0.0361	0.2469	14.7489	38.2409	-46.2168
33	1.8214	-0.3698	-0.4646	1.6354	0.0953	0.9197	0.1145	0.4363	0.0899	-0.4767	-0.0655	-0.1601	-0.1845	0.1674	-0.0536	0.4065	-0.0013	0.0887	0.4193	0.0104	0.23	17.4076	28.6723	-52.6811
34	3.3031	0.5819	-0.2387	2.3651	-0.281	1.8046	0.1234	0.391	0.045	-0.4431	-0.0956	-0.397	-0.1241	0.3478	-0.0014	0.3153	0.0511	0.0751	0.3468	-0.0005	0.2436	18.2166	23.8215	-50.6894
35	0.4434	-0.0663	0.6578	0.7535	-0.4058	2.5488	-0.0256	0.0541	-0.0106	-0.0377	0.0205	0.1888	-0.2444	-0.1899	0.0106	0.2419	0.0483	-0.0334	0.273	-0.0476	0.2546	-21.3256	25.5922	-53.9045
36	2.3245	0.7781	0.2466	1.6326	-0.3325	2.0182	0.0671	0.0011	0.0576	-0.0448	-0.0488	0.2845	-0.0968	-0.296	-0.0104	0.2035	0.0202	-0.0296	0.2251	-0.023	0.2378	-22.6493	28.0026	-31.5982
37	2.0001	3.5712	4.7361	5.5172	-0.6278	5.0346	-0.4762	-0.1438	0.1887	-0.969	0.3743	1.3266	-0.091	0.5716	0.0956	0.3592	0.0691	-0.0668	0.3343	-0.0566	0.2896	-3.1056	19.1694	-47.7033

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 6 through 45 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 46 through 112 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 113 through 134 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 135 through 165 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 166 through 215 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 216 through 350 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 351 through 383 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 384 through 424 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 425 through 469 )
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 502 through 502 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 9 through 34 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 35 through 63 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 64 through 112 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 113 through 150 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 151 through 215 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 216 through 350 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 351 through 383 )
18	X-RAY DIFFRACTION	18	chain 'B' and (resid 384 through 424 )
19	X-RAY DIFFRACTION	19	chain 'B' and (resid 425 through 469 )
20	X-RAY DIFFRACTION	20	chain 'B' and (resid 503 through 503 )
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 10 through 165 )
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 166 through 215 )
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 216 through 383 )
24	X-RAY DIFFRACTION	24	chain 'C' and (resid 384 through 469 )
25	X-RAY DIFFRACTION	25	chain 'C' and (resid 502 through 502 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 8 through 34 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 35 through 63 )
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 64 through 112 )
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 113 through 150 )
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 151 through 215 )
31	X-RAY DIFFRACTION	31	chain 'D' and (resid 216 through 279 )
32	X-RAY DIFFRACTION	32	chain 'D' and (resid 280 through 305 )
33	X-RAY DIFFRACTION	33	chain 'D' and (resid 306 through 350 )
34	X-RAY DIFFRACTION	34	chain 'D' and (resid 351 through 383 )
35	X-RAY DIFFRACTION	35	chain 'D' and (resid 384 through 424 )
36	X-RAY DIFFRACTION	36	chain 'D' and (resid 425 through 469 )
37	X-RAY DIFFRACTION	37	chain 'D' and (resid 502 through 502 )

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